中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (16): 2916-2920.doi: 10.3969/j.issn.1673-8225.2010.16.017

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

新型抗菌不锈钢微螺钉种植体的细胞毒性分析

张  丹1，张  扬1，卢  利2，杨  柯3，薛  楠1   

  1. 中国医科大学口腔医学院，1正畸科， 2口腔颌面外科，辽宁省沈阳市  110002；3中国科学院金属研究所，辽宁省沈阳市  110016
  • 出版日期:2010-04-16 发布日期:2010-04-16
  • 通讯作者: 张 扬，教授，主任医师，中国医科大学口腔医学院正畸科，辽宁省沈阳市 110002
  • 作者简介:张 丹★，女，1979年生，辽宁省辽阳市人，汉族，2005年中国医科大学毕业，硕士，讲师，主治医师，主要从事口腔正畸临床、科研、教学工作。 dan20040326@sohu.com

Cytotoxicity of a new type of antibiotic stainless micro-screw implant

Zhang Dan1, Zhang Yang1, Lu Li2, Yang Ke3, Xue Nan1   

  1. 1 Department of Orthodontics, School of Stomatology, China Medical University, Shenyang  110002, Liaoning Province, China; 2 Department of Oral and Maxillofacial Surgery, School of Stomatology , China Medical University, Shenyang  110002, Liaoning Province, China; 3 Institute of Metal Research, Chinese Academy of Sciences, Shenyang  110016, Liaoning Province, China
  • Online:2010-04-16 Published:2010-04-16
  • Contact: Zhang Yang, Professor, Chief physician, Department of Orthodontics, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China
  • About author:Zhang Dan★, Master, Lecturer, Attending physician, Department of Orthodontics, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China dan20040326@sohu.com

PDF

471

被引次数

16

摘要：

背景：降低不锈钢微螺钉种植体的脱落率，开发新型的抗菌材料，可以从根本上预防种植体周围炎的发生。
目的：通过体外细胞培养法评价新型抗菌不锈钢种植体的细胞毒性。
方法：将待检试件(抗菌不锈钢、医用不锈钢、医用纯钛)制备成15 mm×10 mm×3 mm的长方体，表面逐级抛光后，无水乙醇脱脂、超声清洗并行高温高压消毒。将各试件以3 cm2/mL的比例浸泡于DMEM高糖培养基中置于37 ℃温箱内96 h，微孔滤膜过滤除菌。实验设0.64%的苯酚培养液作为阳性对照组及空白DMEM培养基阴性对照组。倒置相差显微镜观察MG63细胞在各试件浸提液中的生长情况，通过MTT实验得出细胞在培养24，48，72，96，120 h后的吸光度值，并计算其相对增殖率评价抗菌不锈钢的细胞毒性。
结果与结论： ①培养24 h，阳性对照组细胞形态异常呈空泡状、其余各组细胞贴壁生长良好。②培养48 h后，随着作用时间的延长，阳性对照组细胞表现出明显的中毒形态，其余各组细胞生长旺盛，医用不锈钢及抗菌不锈钢组开始出现少量细胞核固缩。③3组试件吸光度值由高到低依次为医用纯钛、抗菌不锈钢、医用不锈钢，但3者之间差异无显著性意义。④在观察期医用纯钛组、医用不锈钢组、抗菌不锈钢组细胞毒性等级均为0级。结果表明抗菌不锈钢毒性等级与医用不锈钢和医用纯钛相同，符合口腔正畸材料的临床应用要求。

关键词: 抗菌不锈钢, 细胞毒性, MTT, 种植体, 浸提液, 生物材料

Abstract:

BACKGROUND: Prevention of implant from inflammation was an effective method to reduce expulsion rate of stainless steel micro-screw implant, and develop new type of antibiotic material.
OBJECTIVE: To evaluate the cytotoxicity of a new type of antibiotic stainless steels.
METHODS: Metal test samples (antibiotic stainless steel, medical stainless steel, and medial pure titanium) were made into rectangular solids with length of 15 mm × 10 mm × 3 mm. Samples were cleaned with high temperature and high pressure. Alloy leaching liquor was prepared with DMEM culture media according to the ratio between surface area and volume of culture solution (3 cm2/mL). The leaching liquor was maintained in incubator at 37 ℃ for 96 hours, and then degerming was performed using microporous membrane. 6.4% phenol was added, which was considered as the positive control group, and DMEM culture media was considered as the blank control group. Growth of MG63 cells was observed under inverted phase contrast microscope; absorbance of cells cultured for 24, 48, 72, 96, and 120 hours was detected using MTT test; cytotoxicity of antibiotic stainless steels was evaluated.
RESULTS AND CONCLUSION:  ① At 24 hours after culture, cells in the positive control group was abnormal; while, cells in other groups were well adherent-grew. ② After 48 hours of culture, with the culture time increased, cytotoxicity was detected out in the positive control group; cells in other groups and blank control groups were normal and grew well. A few of cells in stainless steels group showed karyopyknosis. ③ The absorbance was the highest of medical pure titanium, and then of antibiotic stainless steel and of medical stainless steel, while there was no significant difference between the three materials. ④ The level of cytotoxicity was grade 0. The results suggested that the antibiotic stainless steel which had the same cytotoxicity grade as medical stainless steel and pure titanium was in line with the requirement of its clinical application.

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