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    12 February 2015, Volume 19 Issue 7 Previous Issue    Next Issue
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    Reduced glutathione promotes the proliferation and differentiation of osteoblasts under high glucose conditions
    Zhang Lin-lin, Zhang Jing-ying, Qu Zhe, Song Ke-dong, Ai Hong-jun
    2015, 19 (7):  985-990.  doi: 10.3969/j.issn.2095-4344.2015.07.001
    Abstract ( 323 )   PDF (983KB) ( 782 )   Save

    BACKGROUND: Diabetes can reduce the success rate of implants by affecting osteogenesis. What’s more, it also causes a variety of complications. Reduced glutathione can remove free radicals directly or indirectly by an enzymatic reaction, and thus plays a protective role on osteoblasts.

     
    OBJECTIVE: To evaluate the effects of reduced glutathione on the proliferation and differentiation of osteoblasts under high glucose conditions and the influence on bone defects of diabetes rabbits.
    METHODS: In this experiment, the osteoblasts were divided into three groups: normal control group, high glucose group, high glucose+reduced glutathione group. We observed the proliferation and differentiation of osteoblasts by alkaline phosphatase staining and MTT. The diabetes rabbits which had bone defects were divided into two groups, and the mixture of reduced glutathione and Bioss was used to fill the bone defects in the experimental group to judge the influence of reduced glutathione on osteogenesis based on X-ray observation.
    RESULTS AND CONCLUSION: Reduced glutathione could effectively promote the proliferation and differentiation of osteoblasts under high glucose conditions. The cell proliferation and differentiation showed more significant increase in the high glucose+reduced glutathione group than the high glucose group (P < 0.05). The bone mineral density shadow of the tibial bone defects after treatment with the mixture of reduced glutathione and Bioss was significantly higher than that after treatment with single Bioss (P < 0.05). These findings indicate that reduced glutathione can promote the proliferation and differentiation of osteoblasts, and effectively promote the repair of bone defects.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Age difference of estrogen receptor gene polymorphisms in the elderly women with hip osteoporosis
    Wang Zeng-rong
    2015, 19 (7):  991-995.  doi: 10.3969/j.issn.2095-4344.2015.07.002
    Abstract ( 271 )   PDF (314KB) ( 411 )   Save

    BACKGROUND: Since the age differences exist in gene polymorphism, the incidence of osteoporosis-induced systemic skeletal diseases is varied in different age groups.

     
    OBJECTIVE: To investigate the age differences of estrogen receptor gene polymorphisms in the elderly women with hip osteoporosis.
    METHODS: Seventy-two elderly women with hip osteoporosis were selected as osteoporosis group, and another 72 non-hip osteoporosis elderly women served as controls. There was no significant difference in age and diseases between two groups (P > 0.05). Simultaneously, PCR-RFLP at loci of Xba I and Pvu II and bone mineral density were analyzed and tested.

    RESULTS AND CONCLUSION: The distribution rates of X and XX alleles were significantly higher in the osteoporosis group than the control group (P < 0.05). Patients with the XX genotype were significantly older than those with Xx and xx genotypes (P < 0.05). Logistic regression linear correlation analysis showed that the age was obviously correlated with the occurrence of hip osteoporosis in patients with XX genotypes (P < 0.05). These findings indicate that gene polymorphisms at XX locus of estrogen receptor gene have some influences on the occurrence of hip osteoporosis in elderly women, and there is the age-related distribution difference.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Three-dimensional microarchitecture of the proximal femur in osteoarthritis and rheumatoid arthritis
    Wang Bai-liang, Ming Ding, Søren Overgaard
    2015, 19 (7):  996-1002.  doi: 10.3969/j.issn.2095-4344.2015.07.003
    Abstract ( 252 )   PDF (496KB) ( 964 )   Save

    BACKGROUND: The main reason for reducing the life of joint prosthesis is prosthetic loosening. In addition to prosthesis design, surgical technique, prosthetic material and the resulting wear particles, bone quality also plays a very important role in prosthetic loosening. Bone tissue microstructure has an important impact on bone quality. Recently, the quantification of bone architecture based on micro-CT has been widely used in the research of various bone diseases.
    OBJECTIVE: To observe the osteoarthritis- and rheumatoid arthritis-related changes in the properties of the proximal femur based on micro-CT, thus to compare the bone microstructure in osteoarthritis and rheumatoid arthritis patients.
    METHODS: Femoral heads were collected from primary osteoarthritis (n=10) and rheumatoid arthritis (n=7) patients undergoing total hip replacement. A 10-mm segment of the femoral neck was cut from each individual femur, perpendicular to the main trabecular direction on X-ray films. The specimens were analyzed by using micro-CT system. After scanning, the data were transferred to three-dimensional images, and then detailed structural parameters of the cortical bone, cancellous bone and femoral neck were statistically analyzed based on novel unbiased, model-free three-dimensional methods.
    RESULTS AND CONCLUSION: There was no significant difference in the microstructure of the femoral head (cortical bone, cancellous bone and the entire) between the primary osteoarthritis and rheumatoid arthritis groups. The overall microstructure properties of the femoral neck were similar to those of the cancellous bone. Primary osteoarthritis patients were characterized by a more loss of the connectivity to the trabecular bone, an increase in degree of anisotropy for the cortical bone, but a decrease in degree of anisotropy for the cancellous bone and the entire trabecular bone, when compared to the rheumatoid arthritis group. These findings show that there is no difference in the microstructure of the cortical bone, the cancellous bone and the entire femoral neck between patients with primary osteoarthritis and rheumatoid arthritis, suggesting that primary osteoarthritis and rheumatoid arthritis have a similar trend of global microarchitectural degeneration in the femoral neck.

    中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

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    Ginsenoside Rg1 effect on proliferation of spinal neurons
    Sun Jian-zhong,Liu Xin-wei, Guan Hua-peng, Zhang Peng, Liu Qi, Yang Jun, Guo Qun-feng, Ni Bin
    2015, 19 (7):  1003-1007.  doi: 10.3969/j.issn.2095-4344.2015.07.004
    Abstract ( 367 )   PDF (481KB) ( 573 )   Save

    BACKGROUND: Different neuroreceptors medicated by different receptors produce their own biological functions. The root cause of spinal cord injury is the death of neurons and the synaptic dysfunction. Ginsenoside Rg1 may play a protective role in neurons.

     
    OBJECTIVE: To verify the nutritional and protective roles of ginsenoside Rg1 in spinal neurons of Sprague-Dawley rats.
    METHODS: The spinal neurons were isolated from embryonic rats at a gestation age of 16 days, and following cryopreservation and resuscitation, the cells were divided into three groups: blank group, ginsenoside Rg1 group and positive control group (phenol solution). MTT method was used to detect the activity of mitochondria and ginsenoside Rg1 effects on the neuronal proliferation.
    RESULTS AND CONCLUSION: Compared with the blank group, the cell absorbance value and cell relative activity were both higher in the ginsenoside Rg1 group, indicating that ginsenoside Rg1 can encourage the growth of neurons. When cells were intervened by ginsenoside Rg1, cell processes grew faster than those in the blank group, indicating ginsenoside Rg1 can protect the neurons, inhibit neuronal apoptosis, and boost the cell vitality.


    中国组织工程研究
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    A novel role of cyclin-dependent kinase PFTK1 in the differentiation of oligodendrocyte precursor cells
    Yang Hai-jie, Cui Yang-yang, Wang Lei, Ma Shuang-ping, Li Zhi-chao, Feng Hui-gen
    2015, 19 (7):  1008-1012.  doi: 10.3969/j.issn.2095-4344.2015.07.005
    Abstract ( 330 )   PDF (531KB) ( 680 )   Save

    BACKGROUND: Oligodendrocytes are mainly differentiated from oligodendrocyte precursor cells and the differentiation is tightly regulated by various factors. Some cyclin-dependent kinases play pivotal roles in oligodendrocyte precursor cell differentiation due to their regulation on the early stage of differentiation.

    OBJECTIVE: To investigate the potential role of a newly identified cyclin-dependent kinase-like protein, PFTK1 in differentiation and also study the related mechanisms.
    METHODS: siRNA technology and western blot assay were used to examine the differentiation markers of OLN-93 and the activation condition of corresponding signal pathways.
    RESULTS AND CONCLUSION: With serum-deprivation, OLN-93 underwent differentiation simultaneously. When PFTK1 was silenced by siRNA technology, OLN-93 differentiation was significantly improved, as judged by morphological observations. Accordingly, western blot results showed a marked increase of differentiation markers cyclic nucleotide phosphodiesterase and myelin oligodendrocyte glycoprotein in PFTK1-silenced cells. From the study on PFTK1-related signaling pathways, we found that PFTK1 silencing induced the activation of PI3K/AKT pathway but not MAPK/ERK pathway. Furthermore, the inhibition of AKT by its specific inhibitor abrogated PFTK1 silencing-promoted OLN-93 differentiation. Taken together, our data demonstrate that PFTK1 negatively regulates OLN-93 differentiation through PI3K/AKT pathway, providing a clue for developing new therapeutic approaches to treat degenerative neurological diseases related to demyelination.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Clinical significance of bone metabolic markers in the diagnosis and treatment of senile osteoporosis
    Hu Xiao-jun, Kuang Rong-bin
    2015, 19 (7):  1013-1017.  doi: 10.3969/j.issn.2095-4344.2015.07.006
    Abstract ( 501 )   PDF (317KB) ( 705 )   Save

    BACKGROUND: Osteoporosis is not a simple quantitative bone problem. Assessment of osteoporosis is related to age, gender, bone metabolism, bone formation and bone biomechanical dynamics. Tartrate-resistant acid phosphatase 5b (TRACP 5b) and bone alkaline phosphatase (BAP) are two important indicators reflecting bone resorption and bone formation, respectively.

    OBJECTIVE: To investigate the clinical significance of bone metabolic markers in the diagnosis and treatment of senile osteoporosis, and to explore its correlation with bone mineral density changes.
    METHODS: Totally 100 elderly patients with osteoporosis were enrolled, including 30 cases of 65-70 years old, 40 cases of 71-75 years old, and 30 cases of 76-80 years old. Patients with diabetes, cancers and other bone disorders were excluded. At admission, all patients were measured four bone biochemical markers, including TRACP 5b, BAP, N-terminal midfragment osteocalcin and 25-hydroxyvitamin D. According to the test values of TRACP 5b and BAP, patients were divided into TRACP 5b upregulation group and BAP upregulation group. The correlation of bone mineral density and four biochemical markers with sex and age was analyzed, and intergroup and intragroup differences in four biochemical markers were compared.
    RESULTS AND CONCLUSION: The bone mineral densities of the lumbar vertebra and hip were decreased with age  (P < 0.05); the bone mineral densities of the lumbar spine and Ward’s triangle in the male patients were higher than those in the female patients. The values of TRACP 5b, BAP, N-terminal midfragment osteocalcin were increased with age (P < 0.05), while the level of 25-hydroxyvitamin D was decreased with age (P < 0.05). Compared with the male patients, the levels of TRACP 5b, BAP and N-terminal midfragment osteocalcin were higher in the female patients, but the level of 25-hydroxyvitamin D was lower in the female patients. After anti-osteoporosis treatment, these four biochemical markers were all improved in the two groups. These findings indicate that the detection of bone metabolic markers contributes to explain whether the main function of osteoblasts or osteoclasts is present in the body, thereby guiding the clinical use of targeted anti-osteoporosis drugs and providing a scientific basis for the clinical treatment and monitoring of osteoporosis.


    中国组织工程研究
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    Morphological characteristics of the incisive alveolar bone in adolescents with skeletal class II malocclusion of different vertical facial skeletal types
    Ruan Xiao-hui, Li Liang, Wu Pei-ling
    2015, 19 (7):  1018-1022.  doi: 10.3969/j.issn.2095-4344.2015.07.007
    Abstract ( 384 )   PDF (322KB) ( 827 )   Save

    BACKGROUND: In the process of tooth movement, alveolar bone remodeling process is not only influenced by orthodontic force approach and balance between lip and tongue, but also limited by alveolar bone morphology.

    OBJECTIVE: To preliminarily study the morphological characteristics of the incisive alveolar bone in adolescent with skeletal class II malocclusion of different vertical facial skeletal types.
    METHODS: Sixty patients with class II malocclusion, aged 11-16 years, were divided into three groups: (1) high-angle group: the angle between the anterior skull base and mandibular plane was > 40°, and the angle between the orbitomeatal plane and mandibular plane angle was > 32°. (2) Low-angle group: the angle between the anterior skull base and mandibular plane was < 29°, and the angle between the orbitomeatal plane and mandibular plane angle was < 22°. (3) Average angle group: the angle between the anterior skull base and mandibular plane was ≥ 29°, ≤ 40°, and the angle between the orbitomeatal plane and mandibular plane angle was ≥ 22°, ≤ 32°. Each group included 20 subjects. Cephalometric films were taken and measured to analyze the morphological characteristics of the incisive alveolar bone with SPSS 17.0.
    RESULTS AND CONCLUSION: (1) The thickness of the apical alveolar: the UBP, LBP value and the total thickness of the apical alveolar were significantly different between the three groups (P < 0.05). The ULP and LLP values were significant different between the high-angle and low-angle groups (P < 0.05). (2) The alveolar height: the UBH, ULH, LBH, and LLH values showed significant differences among the three groups (P < 0.05). These findings indicate that the thickness of the labial alveolar bone of the incisor is obviously thinner in the high-angle malocclusion than others, indicating it is very important to prevent the fenestration and bone cracking. The labial alveolar bone of the low-angle malocclusion is thicker, and therefore, the adduction range of the anterior teeth is wider.


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    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Selective anterior cruciate ligament bundle reconstruction using autologous hamstring tendon under arthroscopy
    Zhang Yong
    2015, 19 (7):  1023-1027.  doi: 10.3969/j.issn.2095-4344.2015.07.008
    Abstract ( 464 )   PDF (552KB) ( 419 )   Save

    BACKGROUND: Knee cruciate ligament injuries are often partial injuries of the ligaments, and there are some normal parts of the ligament that can be retained. But the damage has led to joint instability and produced clinical symptoms, the complete ligament resection and reconstruction is bound to cause greater injuries.

     
    OBJECTIVE: To investigate the effect of selective anterior cruciate ligament bundle reconstruction using autologous hamstring tendon under arthroscopy on the function of knee joint.
    METHODS: Forty patients with section beam fracture of the anterior cruciate ligament undergoing arthroscopic selective reconstruction were enrolled and followed for 6-48 months to evaluate the function of knee joint before and after treatment.
    RESULTS AND CONCLUSION: Compared with the preoperative data, the positive rates of Lachman test, anterior drawer test and ADT test, Tegner score, and tibial displacement distance were all decreased significantly after treatment (P < 0.05), but IKDC2000, Lysholm, BRISTOL scores were increased significantly (P < 0.05). Selective anterior cruciate ligament bundle reconstruction under arthroscopy can effectively control patient’s conditions and relief symptoms, postoperative functional recovery of knee joint is good, and it is conducive to maintaining the stability of knee joint.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Conjoined tendon and coracoacromial ligament transfer in the treatment of RockwoodIII acromioclavicular joint dislocation: anatomical and clinical validation
    Wang Zhi-zhou, Yilihamu Tuoheti, Xu Chao, Hou Yan-jie, Guo Hong-liang, Gan Zi-ming
    2015, 19 (7):  1028-1035.  doi: 10.3969/j.issn.2095-4344.2015.07.009
    Abstract ( 456 )   PDF (1356KB) ( 491 )   Save

    BACKGROUND: There are many surgical methods for treatment of acromioclavicular joint dislocation. Through various internal fixation materials (such as hook plate, screws, K-wire, etc.), we can achieve the initial stability and restoration of the acromioclavicular joint. But these internal fixators can cause varying degrees of occupancy and damage to the acromioclavicular joint, and the joint reduction often miss after removal of the internal fixators.

     
    OBJECTIVE: To use conjoined tendon and coracoacromial ligament transfer for coracoclavicular ligament reconstruction in the repair of RockwoodIII acromioclavicular joint dislocation, to provide the theoretical foundation through the autopsy, and to analyze the clinical efficacy based on clinical follow-up results.
    METHODS: (1) Autopsy morphology research: From September 2012 to November 2012, totally 46 adult cadaveric human shoulders were dissected in the Department of Anatomy, Xinjiang Medical University. The anatomical morphology of the acromioclavicular joint was observed and the relevant morphological parameters of the ligament were measured. (2) Case analysis: Eleven patients who received coracoclavicular ligament reconstruction with the lateral half of the conjoined tendon and medial half of the coracoacromial ligament for repair of RockwoodIII acromioclavicular joint dislocation from 2012 to 2014 were followed up. At the last follow-up visit, postoperative radiographic evaluation was done for the acromioclavicular joint recovery, and the American Shoulder and Elbow Surgeons (ASES) scores, Constant-Murley shoulder scores and UCLA shoulder scores, Simple Shoulder Test Form were adopted to evaluate the shoulder functions; visual analog scale scores were used for pain evaluation.
    RESULTS AND CONCLUSION: (1) Results of autopsy morphology research: Detail morphology data of the acromioclavicular joint, its surrounding tissues and musculocutaneous nerve were given, which provide the anatomical data for operation at this region. (2) Result of case analysis: After 2-24 months of follow-up, the mean ASES score was 92.3, the mean Coustant-Murley score was 90.4, the mean UCLA shoulder score was 31.6, and the mean visual analog scale score for pain was 1.4. The number of positive answer to the Simple Shoulder Test Form was 6, and the overall excellent rate was 90.9% (10/11). Poor results were found in one case. Through the anatomical reconstruction of the static stability (such as coracoclavicular ligaments) and dynamic stability (such as joint capsule, trapezius muscle and deltoid muscle) of the acromioclavicular joint, the anatomical reduction of the acromioclavicular joint can be implemented. In a word, the double-beam coracoclavicular ligament reconstruction using the conjoined tendon and coracoacromial ligament is an effective method to repair RockwoodIII acromioclavicular joint dislocation.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Atrial fibrosis and tissue remodeling: the role of transforming growth factor-beta1 and other neurohormonal media
    Yuan Xiao-fei, Xu Quan-fu, Li Yi-gang
    2015, 19 (7):  1036-1041.  doi: 10.3969/j.issn.2095-4344.2015.07.010
    Abstract ( 337 )   PDF (366KB) ( 386 )   Save

    BACKGROUND: More and more studies have shown that atrial fibrosis is caused by the interaction of multiple neurohormonal media, including transforming growth factor-β1, rennin-angiotensin-aldosterone system, galectin 3, inflammatory mediators, relaxin.

    OBJECTIVE: To investigate the relationship between these neurohormonal media and atrial fibrosis as well as the role in the occurrence and development of atrial structural remodeling and atrial fibrillation.
    METHODS: PubMed and CNKI databases were searched by computer for relevant articles published from 2000 to 2014. The keywords were “atrial fibrosis, structural remodeling, atrial fibrillation, transforming growth factor-β1, rennin-angiotensin-aldosterone, galectin-3, inflammation, relaxin” in Chinese and “myocardial fibrosis, structural remodeling, atrial fibrillation, RAAS, galectin-3, inflammatory mediators, relaxin” in English. According to the inclusion and exclusion criteria, 46 articles were included finally.
    RESULTS AND CONCLUSION: By analyzing the many factors that influence atrial fibrosis, we can find that the factors promoting fibrosis are not absolutely isolated, but mutually enhance or inhibit each other in a certain manner, and wherein the transforming growth factor-β1 plays a central role in various mechanisms. Various signaling pathways have different emphases, and there is also a cross-interaction among these signaling pathways deepens the complexity of the pathogenesis of atrial fibrosis. Atrial fibrosis can lead to conduction heterogeneity in the local area, and induce unidirectional conduction block and multiple wavelet reentry, thereby developing atrial fibrillation.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Effects of connective tissue growth factor on wound healing and scarring
    He Ze-liang, Tang Yong, Yao Zong-jiang, Zhang Xuan-fen
    2015, 19 (7):  1042-1046.  doi: 10.3969/j.issn.2095-4344.2015.07.011
    Abstract ( 405 )   PDF (478KB) ( 612 )   Save

    BACKGROUND: The wound healing is the hot topic in the research of surgery. Connective tissue growth factor plays an important role in the regulation of wound healing.

    OBJECTIVE: To review the current development of connective tissue growth factor in wound healing, and to investigate the mechanism.
    METHODS: The CNKI database, WANFANG database, VIP database, Springer Link database and PubMed database were retrieved for the related articles with the key words of “CTGF; CCN2; scar; wound healing; regeneration” in English and Chinese, respectively. A total of 37 articles related to the basic study of connective tissue growth factor involved in the regulation of wound healing were included.
    RESULTS AND CONCLUSION: Connective tissue growth factor has dynamic expression in skin wound healing and scar formation. In the early stage of wound repair, the transient up-regulation of connective tissue growth factor can accelerate the wound healing by promoting the formation of granulation tissue and new blood vessels, while its sustained expression in wound repair and remodeling phases can promote excessive produce of matrixes, collagen deposition and scar contracture, thereby leading to the formation of pathologic scars. Connective tissue growth factors are involved in the skin wound healing, which have broad application prospects in accelerating wound healing and inhibiting scar formation. Connective tissue growth factor as the downstream product of transforming growth factor-β, in addition to its own biological role, can mediate transforming growth factor β contributing to the proliferation of fibroblasts, promoting fibrosis, induced adhesion and migration, which


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    Ozone treatment of nonalcoholic fatty liver: variation of cytokine levels
    Fan Xiao-tang, Halida Xiaerfuhazi, Chen Xin-chun, Ma Hai-lin, Shi Xiu-jiang, He Fang-ping
    2015, 19 (7):  1047-1051.  doi: 10.3969/j.issn.2095-4344.2015.07.012
    Abstract ( 474 )   PDF (356KB) ( 451 )   Save

    BACKGROUND: Now, ozone autologous blood reinfusion treatment has been used as a supplementary treatment or auxiliary treatment besides guidelines. But the relevant reports often address the descriptive observation of clinical efficacy, and whether ozone treatment can influence the corresponding serum cytokines is unclear.

    OBJECTIVE: To observe the effects of ozone autologous blood reinfusion treatment in nonalcoholic fatty liver patients by testing serum nitric oxide, prostaglandin F-2a, interleukin-6, high-sensitive C-reactive protein and oxidized low-density lipoprotein and leptin.
    METHODS: Thirty-six hospitalized patients with non-alcoholic fatty liver disease were enrolled, and according to the wishes of the patients, they were divided into ozone treatment group and control group. The control group was only treated by the liver protective drug treatment, and the treatment group was treated with ozone autologous blood reinfusion treatment besides the liver protective drug treatment. All patients with type 2 diabetes and (or) dyslipidemia received professional hypoglycemic and (or) lipid-lowering therapy. Prostaglandin F-2a, interleukin-6, high-sensitive C-reactive protein, oxidized low-density lipoprotein and leptin levels were compared before and after treatment.
    RESULTS AND CONCLUSION: Compared with the control group, the serum levels of prostaglandin F-2a and leptin levels were significantly higher in the treatment group (P ≤ 0.05) at baseline, but after treatment, serum leptin levels had no significant difference between the two groups (P > 0.05), and serum prostaglandin F-2a levels were still statistically different between the two groups (P=0.02). These findings suggest that the ozone autologous blood reinfusion treatment can reduce the serum leptin levels and improve leptin resistance in patients with nonalcoholic fatty liver. Therefore, this treatment may have prevention and treatment effects on the long-term prognosis of nonalcoholic fatty liver disease patients.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Microsatellite instability of 10 human cancer cell lines
    Shi Zhong-zheng, Liu Yan, Luo Min, Chen Ying-jie, Zhou Yi-ping
    2015, 19 (7):  1052-1056.  doi: 10.3969/j.issn.2095-4344.2015.07.013
    Abstract ( 427 )   PDF (434KB) ( 490 )   Save

    BACKGROUND: We aim to understand microsatellite instability in various human cancer cells.

    OBJECTIVE: To analyze the difference in genotypic background of various tissue-derived human cancer cells based on microsatellite instability.
    METHODS: Ten human cancer cell lines at exponential phase were cultured, including human leukemia cells, colon cancer cells, stomach cancer cells, liver cancer cells, lung cancer cells, cervical cancer cells, bladder cancer cells, and two normal human cell lines (human umbilical vein endothelial cells and human skin fibroblasts), and their microsatellite stability statuses were detected by five quasi-monomorphic markers: BAT-25, BAT-26, CAT-25, SEC-63, NR-24. DNA of 10 human cancer cell lines, human umbilical vein endothelial cells and human dermal fibroblasts was extracted and amplified by PCR. PCR products were detected by 12% polyacrylamide gel electrophoresis (PAGE)-silver staining and sequenced by ABI 3720XL automatic sequencer.
    RESULTS AND CONCLUSION: For all the five microsatellite markers detected, the length of their PCR amplified fragments was nearly identical in human umbilical vein endothelial cells and human dermal fibroblasts, demonstrating their quasi-monomorphic nature. When compared with human umbilical vein endothelial cells and human dermal fibroblasts, amplified fragments in human colon cancer cells (HCT-116) and human bladder cancer cells (BIU-87) were shortened for 7-13 bp at BAT-25, BAT-26, CAT-25 or NR-24, respectively. No obvious differences were found at all the five loci in other cells. According to the results, HCT-116 cells and BIU-87 were considered as cells with high-level microsatellite instability. Other cell lines exhibited microsatellite stability at five loci, and they were considered as microsatellite stable cells. Our study revealed status of genome instability in 10 human cancer cell lines, providing references for appropriate cell line selecting in future drug screening and basic medical research.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Activities of reactive oxygen species and superoxide dismutase in human lung adenocarcinoma cells impacted by moxa smoke solution
    Dou Chuan-zi, Wu Huan-gan, Wang Shuo-shuo2, Ma Xiao-peng, Huang Yan, Zhao Ji-meng, Hu Zhi-hai, Liu Hui-rong, Cui Yun-hua, Zhou Ci-li, Zhao Chen
    2015, 19 (7):  1057-1062.  doi: 10.3969/j.issn.2095-4344.2015.07.014
    Abstract ( 501 )   PDF (411KB) ( 568 )   Save

    BACKGROUND: The safety study of moxa smoke has been the focus of attention in the moxibustion research in recent years. As an important direction, the effects of moxa smoke on the human organ, tissue and cells have been discussed. Studies have shown that the solution of moxa smoke can induce apoptosis of human lung adenocarcinoma cells (A549), but the specific mechanism is still unclear.

     
    OBJECTIVE: To observe the influences of moxa smoke on growth inhibition, intracellular reactive oxygen species and superoxide dismutase of A549 cells, and to explore the oxidative damage mechanism of moxa smoke on A549 cells.
    METHODS: Moxa smoke was collected by a portable air sampler, and dissolved in DMEM to different concentrations, as 0.5, 0.4, 0.3, 0.2, 0.1, 0.05, 0.025, 0.01 g/L, which was used to culture A549 cells. Cell Counting Kit-8 method was employed to test cell growth inhibition rate, 2’,7’-Dichlorofluorescin diacetate method was employed to determinate reactive oxygen species intensity, and spectrophotometric method was employed to measure and calculate enzymatic activity of superoxide dismutase.
    RESULTS AND CONCLUSION: The results of different concentrations of smoke solution intervening A549 cells in 4, 6 and 8 hours showed that the concentration of 0.4 g/L and 0.5 g/L on A549 cells growth inhibition rate was higher than 0.01 g/L and 0.025 g/L (P < 0.05). Low concentration of moxa smoke (0.05 g/L and 0.1 g/L) could decrease the intracellular levels of reactive oxygen species, but there was no statistically significant difference between them (P > 0.05); high concentration of moxa smoke (0.4 g/L) could increase intracellular levels of reactive oxygen species (P < 0.01), and decrease the activity of superoxide dismutase (P < 0.01). Moxa smoke is shown to have biological activity on A549 cells, and the mechanism of high-concentration moxa smoke effect on cell growth inhibition may be attributed to peroxidation.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Rapid vascularization of bone tissue engineering
    Bao Xiao-gang, Xu Guo-hua
    2015, 19 (7):  1063-1069.  doi: 10.3969/j.issn.2095-4344.2015.07.015
    Abstract ( 416 )   PDF (496KB) ( 696 )   Save

    BACKGROUND: The main strategies to build rapid vascularization of bone tissue engineering focuse on cell-scaffold or cytokine-scaffold composite construction which has shed light upon the repair of large segmental bone defects.

    OBJECTIVE: To clarify the relevant research progress of the vascularization of bone tissue engineering in the view of the three basic elements of tissue engineering, in order to find out new opportunities for rapid vascularization of bone tissue engineering and to provide inspiration in solving clinically ischemic diseases.
    METHODS: The first author searched the PubMed, Wanfang and VIP databases for relevant literatures published from 1990 to 2014. The keywords were “bone tissue engineering, vascularization, cytokines, coculture, stem cells, osteogenesis, angiogenisis, prevascularization, scaffolds” in English and “bone defect, angiogenesis, vascularized bone tissue engineering, scaffold” in Chinese. After screening, 57 articles were included in result analysis.
    RESULTS AND CONCLUSION: Early vascularization is one of the current bottlenecks of bone tissue engineering development. With the in-depth study of cellular and molecular mechanism of osteogenesis and angiogenisis during natural bone development and the advanced biological scaffolds, cell-scaffold or cytokine-scaffold strategies for rapid vascularization of bone tissue engineering have achieved important achievements. Signal transduction factors acting as “ligand” of angiogenesis or osteogenesis exists individual differences, so we should pay attention to take advantage of stem cells which act as a “donor”; while osteogenesis and angiogenesis are like the two wings of a bird, they are born together and support each other and must function hand in hand, only if they develop coordinately can we realize rapid vascularization of bone tissue engineering. Regulating various cells or cytokines to meet strict spatial patterns and temporal patterns of expression and simulating the physiological state of natural bone are worth exploring to create rapidly vascularized tissue engineered bone.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Small arthrogenous lower back pain combined with knee osteoarthritis: biomechanical significance of inclination angle of the small joints
    Zhao Wen-jie, Zhang Bin, Dai Min
    2015, 19 (7):  1070-1076.  doi: 10.3969/j.issn.2095-4344.2015.07.016
    Abstract ( 371 )   PDF (455KB) ( 410 )   Save

    BACKGROUND: Low back pain is a normal syndrome in department of orthopedics. Moreover, most patients are consisted with knee joint degeneration. Through the conception of “knee-spine syndrome” has been proposed for a long time, the study which focused on low back pain and lumbar facet joints and degenerative knee is not quite easy to find.

    OBJECTIVE: To summarize and analyze articles involved in the low back pain syndrome of small arthrogenous degeneration-induced lower back pain combined with knee osteoarthritis.
    METHODS: We retrieved PubMed database and China National Knowledge Infrastructure for related articles published from January 1998 to August 2014. The key words were low back pain, knee osteoarthritis, lumbar facet joint, total knee replacement, block treatment, non-fusion techniques, inflammatory factor, drug treatment. Repeated articles and some low connected researches were excluded.
    RESULTS AND CONCLUSION: A total of 157 articles were retrieved, and finally 60 articles were included. Present studies showed that small arthrogenous lower back pain could promote osteoarthritis progress. In pathophysiology, tumor necrosis factor-α and interleukin-1β, along with matrix metalloproteinase might play a significant role. As for the biomechanics, the meaning of angle in facet joint, with the inclination angle affected to osteoarthritis is the centre of study. As for clinical treatment, conventional closed treatment in the early stage can identify the source of the pain. For the patients with mild disease, fixation and functional exercises can be used to delay disease progress. In advanced patients, surgical treatment could restore functions and lessen pain.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Role of estrogen in bone metabolism
    Li Hong-liang, Si Jia-wen, Shen Guo-fang
    2015, 19 (7):  1077-1081.  doi: 10.3969/j.issn.2095-4344.2015.07.017
    Abstract ( 420 )   PDF (385KB) ( 654 )   Save

    BACKGROUND: Postmenopausal osteoporosis is a systemic bone metabolic disorder that is closely associated with a decrease in the level of estrogen.

     
    OBJECTIVE: To review the influence of estrogen on bone metabolism by exploring the role of estrogen in osteoblasts and osteoclasts.
    METHODS: A computer-based retrieval of Medline database and CNKI was performed by the first author to search relevant articles addressing the role of estrogen on osteoblasts and osteoclasts using the keywords of “estrogen, osteoporosis, bone metabolism, osteoblasts, osteoclasts, osteogenesis, bone resorption” both in English and Chinese. Totally 216 articles were retrieved, and according to inclusion criteria, and 48 articles were finally retained.
    RESULTS AND CONCLUSION: Estrogen deficiency is associated with a gap between bone resorption and formation, characterized by the loss of bone mass and strength that leads to fragility fractures. Estrogen can regulate the levels of osteoblasts and osteoclasts, thereby inhibiting bone resorption, increasing bone formation, and maintaining bone metabolism.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Effects of neuromuscular electrical stimulation on muscle strength after anterior cruciate ligament reconstruction
    Dai Wen-min, Ding Jie, Zhou Wen, Xu Lin-lin, Li Fang-xiang
    2015, 19 (7):  1082-1086.  doi: 10.3969/j.issn.2095-4344.2015.07.018
    Abstract ( 494 )   PDF (609KB) ( 543 )   Save

    BACKGROUND: Neuromuscular electrical stimulation is a method for promoting motor function recovery by muscle contraction induced by low-frequency pulse current stimulation. It has been verified that neuromuscular electrical stimulation can be used to treat damaged muscle, which can maintain muscle protein synthesis. Conflicting evidence exists regarding the effectiveness of neuromuscular electrical stimulation following anterior cruciate ligament reconstruction.

    OBJECTIVE: To evaluation the clinical efficacy of neuromuscular electrical stimulation on the recovery of muscle strength after anterior cruciate ligament reconstruction.
    METHODS: PubMed database and China National Knowledge Infrastructure were searched by the first author by computer for articles about the effects of different electrical stimulations on muscle strength after anterior cruciate ligament reconstruction published from January 2000 to January 2015. The keywords were “ACL, neuromuscular electrical stimulation” in English and Chinese, respectively. Totally 92 articles were retrieved, but 30 articles met the inclusion criteria.
    RESULTS AND CONCLUSION: Neuromuscular electrical stimulation can improve muscle strength, increase range of motion, reduce edema, reduce muscle atrophy, promote tissue healing and relieve pain, which can also have a positive impact on enzymatic activity in the muscle and can improve patient’s isokinetic muscle strength. The neuromuscular electrical stimulation is preferred after anterior cruciate ligament reconstruction, but its parameter settings for muscle strength recovery are quite different and to choose the right treatment is necessary.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Muscle-specific microRNA-206: research status and prospects
    Zheng Pan-pan, Yu Xin-kai, Zuo Qun, Li Wan-wan, Song Ya-yun
    2015, 19 (7):  1087-1093.  doi: 10.3969/j.issn.2095-4344.2015.07.019
    Abstract ( 471 )   PDF (685KB) ( 705 )   Save

    BACKGROUND: Skeletal muscle accounts for 40% of total body weight, but mechanisms of many skeletal muscle injuries and diseases have not been resolved. MicroRNA-206 (miR-206) is a skeletal muscle-specific microRNA, which plays an important role in skeletal muscle development and regeneration.

     
    OBJECTIVE: To analyze the research status of miR-206 in skeletal muscle injuries and diseases.
    METHODS: A computer-based search of PubMed was performed for articles about microRNA-206 in skeletal muscle injuries and diseases published from 1992 to 2014 using the keywords of “miR-206, skeletal muscle”. Finally, 57 articles were included in result analysis.
    RESULTS AND CONCLUSION: miR-206 can regulate neuromuscular junction recovery after nerve muscle injury. Studies have shown that to control miR-206 levels may be a new method for the treatment of amyotrophic lateral sclerosis and other muscle diseases. miR-206 can promote the differentiation of satellite cells in cell culture, and the lack of miR-206 can delay the differentiation of satellite cells. miR-206 is also found to promote the regeneration of skeletal muscle. After long-term exercise, the miR-206 level shows an adaptive decrease, but if the exercise is stopped for some time, the miR-206 level can return to its original level. Its mechanism remains to be studied.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Double effects of Chemerin on inflammation and metabolic syndrome
    Li Xue-mei, Zhai Li-dong
    2015, 19 (7):  1094-1098.  doi: 10.3969/j.issn.2095-4344.2015.07.020
    Abstract ( 430 )   PDF (391KB) ( 1095 )   Save

    BACKGROUND: In recent years, a number of clinical and basic studies have shown that Chemerin is closely associated with the metabolic syndrome.

     
    OBJECTIVE: Through the review of Chemerin’s role and mechanism in inflammation and obesity, hypertension and lipid metabolism, to summarize the relationship of Chemerin with inflammatory and metabolic syndrome.
    METHODS: Literatures about Chemerin were retrieved in PubMed database and Wanfang database by the first author from 1990 to 2014. Systematic collation, summary and analysis were performed.
    RESULTS AND CONCLUSION: The Chemerin level is associated with body fat content, energy metabolism and inflammation. Chemerin plays an important role in the pathophysiology of obesity and metabolic syndrome. Due to the dual role of Chemerin in inflammation and in metabolism, it may become a key factor linking chronic inflammation, obesity and related diseases. Under different inflammatory states, Chemerin plays different roles, which may be related to different cleavage ways under different inflammatory environments, but Chemerin has no explicit effects in obesity and metabolic syndrome. Further research is needed for the clear relationship of serum levels of Chemerin with obesity and metabolic syndrome.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Ulcerative colitis and epigenetic modification
    Huang Yan, Dou Chuan-zi, Liu Hui-rong, Wu Lu-yi, Lu Yuan, Wang Xiao-mei, Hu Hong-yi
    2015, 19 (7):  1099-1103.  doi: 10.3969/j.issn.2095-4344.2015.07.021
    Abstract ( 401 )   PDF (1066KB) ( 561 )   Save

    BACKGROUND: Ulcerative colitis is found to have a complicated occurrence and development process, which is involved in the response of susceptibility genes to the variation of intestinal microbial internal environmental and other external environmental factors that is able to both induce the body’s innate immune and specific immune protection and tolerance, and lead to chronic inflammation.

    OBJECTIVE: To analyze the progress in the studies about ulcerative colitis and epigenetic modifications in recent 10 years and then to discuss the correlation of susceptibility genes of ulcerative colitis, epigenetic modifications and changes to the occurrence and development of ulcerative colitis.
    METHODS: PubMed and Wanfang databases were searched for relevant articles published between January 2000 and January 2015 using the keywords of “ulcerative colitis, epigenetic, DNA methylation, histone modification, miRNA”. According to the exclusion criteria, 28 articles were included in result analysis.
    RESULTS AND CONCLUSION: The occurrence of ulcerative colitis is primarily associated with susceptibility genes, internal and external environmental factors which are affected by epigenetic modification. Epigenetic modifications include DNA methylation, histone modifications, genes silencing mediated by miRNA. Studies about pathogenic factors of ulcerative colitis show that epigenetic modifications and environmental factors may act in the body’s innate immunity and specific immunity, and epigenetic modification plays a very important role in the occurrence and development of ulcerative colitis.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    A systematic review of 585 nm pulsed dye laser for repair of pathological scars
    Song Li, Ye Jun-ru, Lu Mao, Peng Ke, Shen Yue-li, Zhu Jiang-ting
    2015, 19 (7):  1104-1111.  doi: 10.3969/j.issn.2095-4344.2015.07.022
    Abstract ( 419 )   PDF (333KB) ( 445 )   Save

    BACKGROUND: 585 nm pulsed dye laser is widely used in clinical practice for pathological scars, but the efficacy and safety have not been proved.

     
    OBJECTIVE: To evaluate the effectiveness and safety of 585 nm pulsed dye laser in the treatment of pathological scars.
    METHODS: A computer search of the Cochrane Central Register of Controlled Trials and others was conducted. Meta-analyses were performed for the results of homogenerous studies by Revman 5.2 software.
    RESULTS AND CONCLUSION: Six studies including 208 pathological scars were enrolled. Meta-analyses showed that the 585 nm pulsed dye laser was superior to the blank control group in the change of Vancouver burn scar assessment scale [P < 0.000 01, WMD=-2.79 and 95% CI (-3.12, -2.46)]. 585 nm pulsed dye laser seems to improve the overall texture of the scar; however, it is not confirmed to be effective in the improvement of erythema, size, elasticity and pliability of scars. 585 nm pulsed dye laser is not better than other treatments. It is suggested that it is safe to use 585nm pulsed dye laser in the treatment of pathological scars. It is susceptible to bias due to little sample size.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Myocardial protection of different perfuse ways for cardioplegia in patients undergoing on-pump coronary artery bypass
    Gao Guo-dong, Hu Qiang, Hu Jin-xiao, Duan Xin, Hei Fei-long, Yu Kun, Long Cun
    2015, 19 (7):  1112-1116.  doi: 10.3969/j.issn.2095-4344.2015.07.023
    Abstract ( 340 )   PDF (302KB) ( 480 )   Save

    BACKGROUND: On-pump coronary artery bypass has better long-term effects than off-pump coronary artery bypass. For patients with severe coronary artery stenosis, antegrade perfusion simply via the aortic root is ineffective in myocardial protection, and how to get a good myocardial protection has become one of the key factors affecting the bypass effects.

    OBJECTIVE: To compare the myocardial protection role of different perfuse ways for cardioplegia in the process of coronary artery bypass graft surgery under cardiopulmonary bypass.
    METHODS: Twenty-three patients undergoing coronary artery bypass graft surgery were divided into three groups according to different perfuse ways: antegrade perfusion group, antegrade combined with graft cardioplegia perfusion group and antegrade combined with retrograde perfusion roup. Blood samples were taken to test the levels of serum cardiac troponin I, creatine kinase and MB isoenzyme of creatine kinase before operation, 30 minutes after the beginning of cardiopulmonary bypass, 5 minutes, 2 hours, 6 hours and 24 hours after reperfusion. The amount of vaso-active drugs, postoperative cardiac auto-rebeating rate, cardiopulmonary bypass time, myocardial ischemic time, extubation time and ICU stay time were recorded.
    RESULTS AND CONCLUSION: The serum levels of cardiac troponin I were significantly lower in the cardioplegia perfusion and retrograde perfusion groups than that in the antegrade perfusion group after reperfusion 2 hour to 24 hours (P < 0.05). The levels of creatine kinase were significantly lower in the cardioplegia perfusion and retrograde perfusion groups after reperfusion 5 minutes to 24 hours (P < 0.05). The levels of MB isoenzyme of creatine kinase were significantly lower in the cardioplegia perfusion and retrograde perfusion groups after reperfusion 6 hours to 24 hours  (P < 0.05). There were no significant differences in the postoperative cardiac auto-rebeating rate and the amount of vaso-active drugs used among the three groups. These findings indicate that antegrade combined with graft cardioplegia perfusion or antegrade combined with retrograde perfusion has better protective effects on the myocardium than antegrade coronary perfusion under cardiopulmonary bypass.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Endothelial cell differentiation function varies during telmisartan intervention for hypertension
    Ma Jian, Zhang Yong, Cao Zheng
    2015, 19 (7):  1117-1121.  doi: 10.3969/j.issn.2095-4344.2015.07.024
    Abstract ( 357 )   PDF (304KB) ( 464 )   Save

    BACKGROUND: Studies have shown that in patients with essential hypertension endothelial cell damage is characterized as endothelin release imbalance. Telmisartan can recover the “dipper”-type blood pressure in hypertension patients, and has better antihypertensive effect than losartan and amlodipine, showing a stronger protective role.

    OBJECTIVE: To investigate the effects of telmisartan on endothelial cell differentiation function and serum adiponectin in hypertension patients.
    METHODS: Totally 160 patients with essential hypertension were equally divided into treatment group and control group according to lottery method. The control group was given Hyzaar treatment, while the treatment group received telmisartan treatment, one tablet per day that was taken orally in the morning. The treatment period was 4 weeks. Clinical efficacy, plasma adiponectin, plasma endothelin and nitric oxide levels were observed in the two groups. Correlation between plasma endothelin, plasma adiponectin and nitric oxide levels in patients with hypertension were analyzed.
    RESULTS AND CONCLUSION: The effective rate was 92.5% in the treatment group and 75.0% in the control group, and there was a significant difference between the two groups (P < 0.05). After treatment, the plasma adiponectin and nitric oxide levels were significantly increased, and the plasma endothelin levels were decreased significantly in the two groups, with significant differences compared to pre-treatment (P < 0.05). Compared with the control group, the plasma endothelin level was significantly lower while the nitric oxide level was significantly higher in the treatment group after treatment (both P < 0.05). Pearson correlation test analysis showed that the plasma adiponectin levels were negatively correlated to the plasma endothelin levels (P < 0.05), but positively correlated to the plasma nitric oxide levels (P < 0.05). These findings indicate that telmisartan treatment can improve the prognosis and therapeutic effects on hypertension, and its role may be related to improve endothelial cell differentiation and increase serum adiponectin levels.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Association between SCN5A gene polymorphism and simple congenital heart disease in Xinjiang Uyghur populations
    Song Na, Xu Rui, Zhou Can-lin, Qiu Jin, Reziwanguli Yimin, Zhou Yong
    2015, 19 (7):  1122-1126.  doi: 10.3969/j.issn.2095-4344.2015.07.025
    Abstract ( 355 )   PDF (344KB) ( 448 )   Save

    BACKGROUND: SCN5A is the gene encoding the largest reported cardiac sodium channel, and α subunit of SCN5A gene encoding human cardiac voltage-gated sodium channels is highly expressed in human myocardial cells, which is mainly responsible for generation of action potentials and expansion of excitable cells, and plays an important role in controlling excitability conduction of myocardial cells.

    OBJECTIVE: To explore the correlation between the polymorphism loci of H588R, C5457T, R1193Q of the SCN5A gene and simple congenital heart disease in Xinjiang Uyghur populations.
    METHODS: 150 patients with simple congenital heart disease in Xinjiang Uygur served as case group, and another 150 Xinjiang Uygur healthy people as control group. Polymerase chain reaction-restriction fragment length polymorphism analysis and direct sequencing method were chosen to detect the H588R, C5457T, R1193Q polymorphism loci of SCN5A gene, and to analyze the distribution of different genotypes and allele frequencies in the case group and the control group. 
    RESULTS AND CONCLUSION: The genotype and allele frequencies at polymorphic loci of C5457T and R1193Q were significant different between the case and control groups (P < 0.05). Genotype frequencies of polymorphic locus of H588R were significantly different between the two groups (P < 0.05). Compared with the control group, the polymorphic locus of C5457T in patients with atrial septal defect and patent ductus arteriosus showed significant difference in the allele frequency (P < 0.05), and allele T increased the onset risks for atrial septal defect and patent ductus arteriosus (odd ratio=3.636, 3.467). There were significant differences in allele frequency at the polymorphic loci of R1193Q among patients with atrial septal defect, tetralogy of Fallot and patent foramen ovale (P < 0.05), and allele A increased the onset risks for atrial septal defect, tetralogy of Fallot and patent foramen ovale (odd ratio=3.413, 3.839, 4.059). The polymorphism loci of C5457T, R1193Q of SCN5A gene may be the susceptibility factors for simple congenital heart disease in Xinjiang Uygur populations. The polymorphic locus of H558R is not directly related to simple congenital heart disease in Xinjiang Uygur populations.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Posterior spinal postsurgical infection: infection, obesity and transferring to ICU as independent risk factors
    Na Sen, Xu Tao, Guo Hai-long, Sheng Wei-bin
    2015, 19 (7):  1127-1132.  doi: 10.3969/j.issn.2095-4344.2015.07.026
    Abstract ( 402 )   PDF (298KB) ( 578 )   Save

    BACKGROUND: With the application of new screw-rod system, fusion cage and minimally invasive techniques, the amount of spinal surgeries becomes gradually increasing, along with expanded surgical scope and increased surgical difficulty. Meanwhile, postoperative infection rate is also increasing. Postoperative infections after spinal surgery often lead to increased length of stay and hospital costs, and cause neurological deterioration, and even death.

    OBJECTIVE: To investigate the risk factors and treatment strategies of posterior spinal postoperative infections.
    METHODS: A retrospective analysis including 857 cases of posterior spinal surgery from September 2012 to September 2013 in the Department of Spinal Surgery, the First Affiliated Hospital of Xinjiang Medical University was performed. These patients were divided into infection (n=34) and non-infection (n=823) groups. We compared the preoperative, intraoperative, postoperative factors affecting postoperative infection in the two groups, and summarized the therapeutic strategies by analyzing the treatment methods and therapeutic effects.
    RESULTS AND CONCLUSION: The infection rate after posterior spinal surgery was 3.97% (34/857). There were significantly differences between the two groups in terms of muscle strength ≤ 3 level, preoperative immune dysfunction, acute or chronic infection, obesity, preoperative and postoperative incontinence or catheterization time≥ 3 days, operative time ≥180 minutes, intraoperative bleeding, allogeneic bone grafting, standard use of antibiotics, postsurgical transferring to ICU, the number of drainage pipes, and the time of indwelling drainage tube (P < 0.05), while other variables showed no statistical significance. Logistic regression analysis showed that acute or chronic infections, obesity (bone mass index > 30 kg/m2), transfering to ICU after surgery were independent risk factors for posterior spinal postoperative infections. In addition, individual treatment is preferred for different patients with posterior spinal postoperative infections. It is even more important to choose the appropriate treatment.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Application of vacuum sealing drainage combined with skin flap transplantation in foot soft tissue repair
    Liu Dong, Liang Yun-hai, Dong Ming, Jiang Yan-hui
    2015, 19 (7):  1133-1137.  doi: 10.3969/j.issn.2095-4344.2015.07.027
    Abstract ( 285 )   PDF (372KB) ( 460 )   Save

    BACKGROUND: Foot soft tissue defect can be repaired by skin flap transplantation technology. Vacuum sealing drainage technology can support a good tissue bed for skin flap transplantation, thereby to speed up the local growth of granulation tissue.

    OBJECTIVE: To further verify the application value of vacuum sealing drainage combined with flap transplantation in foot soft tissue repair.
    METHODS: A retrospective analysis was performed on the clinical data of 23 cases of foot soft tissue defects, and these 23 cases were divided into observation group (vacuum sealing drainage combined with flap transplantation; n=12) and control group (flap transplantation with no vacuum sealing drainage; n=11) according to the repair scheme.
    RESULTS AND CONCLUSION: The overall therapeutic excellent rate and survival rate of the flap in the observation group were significantly higher than those in the control group (both P < 0.05). All the 23 patients were followed up for 6 months, and the follow-up rate was 100%. In the observation group, the flaps survived well with red and yellow color, which were soft and had no distinct difference from the surrounding normal skin tissues. The therapeutic effect in the observation group was better than that in the control group. These findings indicate that vacuum sealing drainage combined with flap transplantation in foot soft tissue repair can achieve better effects in the repair of foot soft tissue defects and has certain value in clinical application.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Guided bone regeneration technology used in upper anterior tooth restoration
    Jiang Liu-hong, Dong Ying, Jing Xiang-dong
    2015, 19 (7):  1138-1142.  doi: 10.3969/j.issn.2095-4344.2015.07.028
    Abstract ( 515 )   PDF (362KB) ( 1132 )   Save

    BACKGROUND: The membrane guided bone regeneration technique is based on the mechanical barrier theory of the membrane, which blocks the soft tissue ingrowth into the peri-implant bone defects that make osteoblasts grow well in the space under the membrane to form new bone and fill bone defects.

    OBJECTIVE: To observe the clinical aesthetic effect of guided bone regeneration technique on anterior tooth implantation.
    METHODS: Seventy-four patients with successful single dental implantation were divided into control group and observation group at random, with 37 cases in each group. The control group was treated with phase II anterior tooth implantation with no bone increment. The observation group was treated with anterior tooth implantation implemented by the guided bone regeneration technology, and then bone meal was implanted at initial stable stage after dental implantation. Pink aesthetic indexes and peri-implant bone levels were compared between the two groups on the day after surgery, at 6 and 12 months after implant loading.
    RESULTS AND CONCLUSION: In both groups, the pink aesthetic indexes were significantly increased at 6 and 12 months after implant loading compared with the baseline levels (P < 0.05), but there was no difference between the two groups (P > 0.05). At 12 months after implant loading, in addition to the labial gingival margin levels and alveolar appearance, the rest pink aesthetic indexes were all increased significantly in the two groups compared with the baseline levels (P < 0.05), but there was no difference between the two groups (P > 0.05); there was also no difference in the marginal bone loss between the two groups (P > 0.05). Compared to normal bone implant restoration, the clinical application of guided bone regeneration technique in upper anterior tooth restoration can achieve better results, but there is no significant difference between the two restoration methods. therefore further research and analysis.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    The normal measurements of the upper airway in people from Shandong aged 6-19 years old using cone-beam CT
    Wang Song, Jiang Ying-ying, Hao Jian-zhong
    2015, 19 (7):  1143-1148.  doi: 10.3969/j.issn.2095-4344.2015.07.029
    Abstract ( 394 )   PDF (598KB) ( 383 )   Save

    BACKGROUND: With the extensive application of cone-beam CT in oral and craniofacial surgery, a clear airway indicator for normal population has important implications for the diagnosis and prevention of airway-related abnormalities.

     
    OBJECTIVE: To determine the cone-beam CT measurements of the upper airway (length, width, cross sectional area and volume) in populations aged 6-19 years from Shandong, so as to provide a reference marker for the diagnosis and treatment of upper airway obstruction in local children and adolescents.
    METHODS: Totally 254 healthy children (120 males and 134 females) aged 6-19 years from Shandong were subject to cone-beam CT scan of the upper airway. Mimics10.01 was used to measure the sagittal diameter, transverse diameter, cross-sectional area and volume of nasopharynx, palatopharynx, glossopharyngeal planes, and then the normal value range was confirmed. Independent-sample t-test was used for analysis of gender difference and 95% confidence interval was calculated.
    RESULTS AND CONCLUSION: There were gender differences in a part of indicators of the upper airway among 8-9 years, 10-11 years, 16-17 years, 18-19 years groups. These findings indicate that the normal values of radial lines, cross sectional area and volume of the upper airway as well as 95% confidence interval value provide references for the diagnosis of upper airway obstruction and post-treatment evaluation of anatomic form of the upper airway in local children and adolescents.


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Figures and Tables | References | Related Articles | Metrics