Biocompatibility of nano-hydroxyapatite/collagen/silk fibroin composite scaffolds for bone tissue engineering

doi:10.3969/j.issn.2095-4344.2015.08.001

Abstract

Abstract:

BACKGROUND: Composite scaffolds made of two or more materials can meet the requirements of tissue engineering scaffolds based on the complementary properties of various materials.

OBJECTIVE: To prepare three-dimensional nano-hydroxyapatite (nHA)/collagen (COL)/silk fibroin (SF) scaffolds and to study their biocompatibility.

METHODS: Composite scaffolds were prepared by mixing nHA, COL and SF at different ratios of 1:1:5, 1:2:5, 1:3:5. In order to find out the best mixing ratio of SF, COL and nHA, the porosity, pore size, water absorption and mechanical properties were determined. MC3T3-E1 cells were cultured in the nHA/COL/SF scaffolds composite scaffold (1:2:5) in vitro to observe the biocompatibility. Cell viability was detected after co-culture for 2, 4, 6, 8, 12 days using MTT.

RESULTS AND CONCLUSION: It was more suitable for bone scaffolds when the mixed ratio of nHA, COL and SF was 1:2:5. The pore size was 98-260 μm, the porosity was (96.72±2.78)%, and the water absorption was (549.37±35.29)%. The stability of its mechanical properties, compressive strain and elastic modulus and other indicators were measured by biomechanical testing machine, which are suitable for bone tissue engineering applications. MC3T3-E1 cells grew well in the nHA/COL/SF composite scaffold, indicating the composite scaffold has good cytocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Collagen, Silk, Hydroxyapatites, Tissue Engineering

CLC Number:

R318

Nian Zheng-hao, Li Hui, Li Rui-xin, Sun Kai, Li Dong, Xu Cheng, Zhang Xi-zheng. Biocompatibility of nano-hydroxyapatite/collagen/silk fibroin composite scaffolds for bone tissue engineering[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(8): 1149-1154.

Figures/Tables 3

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