Expression of adiponectin in the periodontal tissue of Kunming mice with diabetic periodontitis

doi:10.3969/j.issn.2095-4344.2015.05.012

Abstract

Abstract:

BACKGROUND: Adiponectin and periodontal disease are closely related with type 2 diabetes.
OBJECTIVE: To detect the expression of adiponectin in periodontal tissue of Kunming mice of diabetes periodontitis, and explore the effects on periodontal tissue pathology and prognosis.
METHODS: Sixty 3-week-old Kunming mice were divided into three groups according to the different modeling methods: control group (normal feeding), diabetes group (diabetic model was established by injection of alloxan), diabetes and periodontitis group (periodontitis model was established by local ligation and bacteria developed). Each group contained 20 rats.
RESULTS AND CONCLUSION: At 20 days after modeling, the blood glucose, fasting insulin and insulin resistance index of mice in the diabetes group were higher than that in the control group (P < 0.05). ELISA, RT-PCR and western blot analysis results showed that, adiponectin gene and protein expression levels in periodontal tissues were the highest in the control group, then in the diabetes group, and the lowest in the diabetes and periodontitis group, at 1, 3, 6, months postoperatively, respectively (P < 0.05). The periodontal gum tissue inflammation and histopathological changes were observed at 1, 3, 6, months postoperatively. The results showed that, the inflammation in periodontal tissues was the most obvious in the diabetes and periodontitis group, and was visible in the diabetes group, but the inflammation was not present in the control group. Experimental findings indicate that, the down-regulation of adiponectin expression in the periodontal tissue is one of the important factors that trigger and aggravate the onset of periodontitis.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Tissue Engineering, Adiponectin, Periodontitis, Diabetes

CLC Number:

R318

Zhao Bo, Wang Yong-lan . Expression of adiponectin in the periodontal tissue of Kunming mice with diabetic periodontitis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(5): 721-726.

Figures/Tables 10

References

[1] 黄宇虹,高秀梅,张伯礼.脂联素的研究进展[J].中国临床药理学与治疗学,2004,9(9):961-965.
[2] 吕莉,冯凭.不同血糖水平人群血清脂联素水平与胰岛素抵抗的关系[J].现代诊断与治疗,2008,19(1):9-11.
[3] 陈幼萍,舒毅,曾春平,等.罗格列酮对2型糖尿病患者高血糖应激状态脂联素的影响[J].中国药师,2010,13(12):1722-1724.
[4] 刘春丽,郭正义,司红卫.脂肪因子chemerin的研究进展[J].泰山医学院学报,2011,32(4):310-314.
[5] 郭伟,张木勋.内质网应激与肥胖、胰岛素抵抗、2型糖尿病[J].医师进修杂志,2005,28(11):56-58.
[6] 赵柯湘,肖谦,刘清秀,等.胰岛素抵抗和2型糖尿病大鼠心肌组织变化的研究[J].四川大学学报(医学版),2008,39(3):402-405.
[7] Weyer C, Funahashi T, Tanaka S, et al. Hypoadiponectim ia in o-besity and type 2 diabetes: close association w ith insulin resis-tance and hyperinsulinemia. J Clin Endocrinol Metab. 2001;86(5):1930-1935.
[8] Hotta K, FunahashiT, A rita Y, et al. Plasma concentration of anove,l A dipose-specific protein,adiponectin, in type 2 diabeticpatients. Arterioscler Thromb Vasc Biol. 2000;20(6): 1595-1599.
[9] 曲建昌,窦京涛.瘦素与肥胖、胰岛素抵抗及2型糖尿病[J].药品评价,2011,8(1):33-36.
[10] 孟焕新.牙周病与全身健康的关系[J].当代医学,2001,7(8): 26-29.
[11] Iacopino AM, Cutler CW. Pathophysiological relationshipsbetween periodontitis and systemic disease: recent con-cepts involving serum lipids. J Periodontol. 2000; 71(8):1375-1384.
[12] 李蓬,张大鹍,张继睿,陈力.伴牙周炎的代谢综合征者动脉硬化早期指标的检测[J].北京大学学报(医学版),2011,43(1):34-39.
[13] 和璐.牙周炎和代谢综合征[J].北京大学学报(医学版),2011, 43(1): 13-17.
[14] 刘国庆,高志,郑华,等.糖尿病大鼠牙周炎症组织中TGF-β1表达的研究[J].重庆医学,2011,40(22):2191-2193, 2196.
[15] 覃双福.糖尿病伴牙周炎患者牙周植骨术87例疗效分析[J].中国医药指南,2011,9(20):114-115.
[16] 夏佳佳,王岚,刘琪,等.2型糖尿病伴牙周炎患者牙周膜干细胞骨向分化研究[J].实用口腔医学杂志,2011,27(5):624-629.
[17] 汪涛,房明,丁鳌,等.牙周基础治疗对Ⅱ型糖尿病伴牙周炎病人糖基化终产物的影响[J].牙体牙髓牙周病学杂志,2011(4):237-239, 209.
[18] 刘艳,普永冰,陆艳蓉.糖尿病患者血清腺苷脱氨酶检测分析[J].检验医学与临床,2011,8(19):2395-2396.
[19] 张婷.2型糖尿病患者的饮食教育探讨[J].检验医学与临床,2011, 8(20):2545-2546.
[20] 赵华,王小泉,赵红宇.2型糖尿病伴牙周炎患者牙周炎症程度与血糖水平分析[J].中华老年口腔医学杂志,2012,10(1):22-24.
[21] 孙运德,果益竹,杨素芳.牙周炎对糖尿病影响的研究进展[J].人民军医,2012(7):670-671.
[22] 王晓平,郭兰荣,杨志芬.牙周基础治疗对2型糖尿病伴牙周炎患者的疗效观察[J].现代实用医学,2012,24(9):1011-1012.
[23] 孙阳,陈树春.内皮祖细胞与脂肪因子[J].中国老年学杂志,2012, 32(6):1312-1314.
[24] Zhu HQ, Yang WY. Prevalence of diabetes in Asia-current status and future. Zhonghua Liuxingbingxue Zazhi. 2011; 32(11):1065-1067.