Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (47): 7608-7614.doi: 10.3969/j.issn.2095-4344.2014.47.012
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Zhang Yong-hu, Song Guo-dong, Zuo Hai-bin, Jia Jun, Ma Yin-dong, Fan Chun-jie, Li Pei-long
Revised:
2014-10-23
Online:
2014-11-19
Published:
2014-11-19
Contact:
Song Guo-dong, Professor, Department of Burn, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong Province, China
About author:
Zhang Yong-hu, Studying for master’s degree, Department of Burn, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong Province, China
Supported by:
the National Key Clinical Specialist Construction Programs of China; Shandong Provincial Technology Development Projects, No. 2013GSF11870; the Medicine and Health Scientific Development Plan of Shandong Province, No. 2011HZ008; Jinan Overseas Students Pioneer Project, No. 20080405
CLC Number:
Zhang Yong-hu, Song Guo-dong, Zuo Hai-bin, Jia Jun, Ma Yin-dong, Fan Chun-jie, Li Pei-long. Comparative analyses of histological and material properties of reticular dermis derived from human, swine and rats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(47): 7608-7614.
2.1 大体观察 3组标本新鲜时均为乳白色,猪边腹部真皮可见大量毛孔,且较为粗大,质地较人和大鼠稍僵硬,伸展性稍差。大鼠网状层真皮质地较为细腻、柔软,也可见较多毛孔,但较细小。人真皮质地在3组之中居中,毛孔较少,伸展性较大。 2.2 生物力学 3组样品复水效果良好,复水后组织呈乳白色,与真空干燥前质地无明显区别。力学检测结果显示:与人网状层真皮比较,猪边腹部网状层真皮的杨氏模量[(29.79±18.82),(36.58±10.79) MPa,P=0.44]、大鼠背部网状层真皮的杨氏模量[(37.99±16.55),(36.58± 10.79) MPa,P=0.36]差异均无显著性意义。 2.3 苏木精-伊红染色 光镜观察结果:猪真皮胶原纤维较为粗大,排列较紧密,毛细血管较人少见且血管直径较小;大鼠真皮胶原较纤细,排列疏松,毛细血管较猪真皮多,与人真皮近似,见图1。 测量结果:猪胶原纤维束直径与人比较差异无显著性意义[(12.30±1.62),(11.74±2.73) μm,P=0.34]。大鼠胶原纤维束直径与人比较差异有显著性意义[(9.69±1.70),(11.74±2.73) μm,P < 0.05],即大鼠真皮胶原纤维比人真皮胶原纤维细;间隙率测量结果显示,人与猪之间差异有显著性意义(P < 0.01),而人与鼠之间差异无显著性意义,即猪真皮较人排列致密,大鼠与人近似(P=0.17),见表1。"
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