Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (47): 7608-7614.doi: 10.3969/j.issn.2095-4344.2014.47.012

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Comparative analyses of histological and material properties of reticular dermis derived from human, swine and rats

Zhang Yong-hu, Song Guo-dong, Zuo Hai-bin, Jia Jun, Ma Yin-dong, Fan Chun-jie, Li Pei-long   

  1. Department of Burn, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong Province, China
  • Revised:2014-10-23 Online:2014-11-19 Published:2014-11-19
  • Contact: Song Guo-dong, Professor, Department of Burn, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong Province, China
  • About author:Zhang Yong-hu, Studying for master’s degree, Department of Burn, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong Province, China
  • Supported by:

    the National Key Clinical Specialist Construction Programs of China; Shandong Provincial Technology Development Projects, No. 2013GSF11870; the Medicine and Health Scientific Development Plan of Shandong Province, No. 2011HZ008; Jinan Overseas Students Pioneer Project, No. 20080405

Abstract:

BACKGROUND: Previous studies of our research group have confirmed that the texture of porcine reticular dermis at lateral ventral part is softer and has more extensibility than other parts. Therefore, it may serve as the raw material of xenogenic acelluar dermal matrix. However, its comparison with human and rat reticular dermis has not been reported systematically in aspects of histomorphology and material characterization.
OBJECTIVE: To compare the reticular dermis from the lateral region of porcine abdomen and rat dorsal part with the reticular dermis from human in histology, biomechanics, molecular structure, thermal stability and other properties.
METHODS: The reticular dermis samples were taken from adult human, the lateral region of porcine abdomen, the back of rats, for gross observation. Paraffin sections were observed by hematoxylin and eosin staining and  sirius red staining under a light microscopy. The relevant data of micrograph were measured by imagine analysis software. These samples were also vacuum-freezing dried and rehydrated, and then their mechanical properties were tested with a electronic tensile machine to calculate the Young’s modulus. Some vacuum-freezing dried samples were powdered and detected by Fourier transform infrared spectrometer and simultaneous thermal analyzer.
RESULTS AND CONCLUSION: There were no differences in collagen fiber bundle diameter of the reticular dermis from adult human and the lateral region of porcine abdomen, but the reticular dermis from the back of rats was thinner than that from adult human (P < 0.01). The gap between the reticular layer of the dermis of the lateral region of porcine abdomen was lower than that from adult human (P < 0.0.1); however, there was no difference in the gap between the reticular layers of the dermis of the rat back and adult human (P=0.17). Collagen fibers of porcine reticular dermis were arranged tightly in disorder; the content of type I collagen and ratio of type I/III collagen were higher than those in the reticular dermis from adult human (P < 0.05), but the content of type III collagen was less than that in the reticular dermis from adult human (P < 0.05). Contents of type I and III collagen and their ratio were similar between the reticular dermis of rats and adult human (P > 0.05). There was no significant difference in the Young’s modulus of the three kinds of reticular dermises. Hydrogen bonds involved in the reticular dermal collagen molecules ranged as follows: rats > swine > human. Rat reticular dermis has better thermal stability than that of swine and adult human.


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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Key words: dermis, swine, persons, rats

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