Cytotoxicity and biocompatibility of nano-hydroxyapatite/bacterial cellulose composite scaffolds

doi:10.3969/j.issn.2095-4344.2014.47.013

Abstract

Abstract:

BACKGROUND: Nano-hydroxyapatite/bacterial cellulose (nHA/BC) composite scaffolds prepared by nano-bionics method exhibit a structure and performance similar to natural bone.
OBJECTIVE: To study the cytotoxicity and biocompatibility of nHA/BC composite scaffolds.
METHODS: (1) Acute systemic toxicity test: nHA/BC leach liquor and normal saline were respectively injected intraperitoneally into Kunming mice. After 24, 48, 72 hours, the body mass of mice was recorded. (2) Sensitization test: nHA/BC leach liquor and normal saline were respectively injected subcutaneously into the back of Japanese white rabbits. With 72 hours of injection, edema and erythema were observed, and the experiment was done again at an interval of 14 days. (3) Pyrogen test: nHA/BC leach liquor and normal saline were respectively injected into the ear vein of Japanese white rabbits, and after injection, the body temperature of rabbits were determined. (4) Hemolysis test: Diluted rabbit anti-coagulant was added into nHA/BC leach liquor, normal saline and distilled water, respectively. (5) Passage 3 bone marrow mesenchymal stem cells from rabbits were co-cultured with nHA/BC materials, and then, cell proliferation, growth and adhesion on the material surface were observed.
RESULTS AND CONCLUSION: nHA/BC composite scaffold was of no acute systemic toxicity, non-allergenic, no pyrogenic and hemolytic reactions; nHA/BC had a three-dimensional porous structure that was better for growth, proliferation and adhesion of rabbit bone marrow mesenchymal stem cells. These findings indicate that the nHA/BC composite scaffold has good biocompatibility and cytocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: nanoparticles, hydroxyapatites, cellulose

CLC Number:

R318

Wang Li-xin, Yuan Feng, Wan Yi-zao, He Xiao-bo. Cytotoxicity and biocompatibility of nano-hydroxyapatite/bacterial cellulose composite scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(47): 7615-7620.

Figures/Tables 5

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