Wnt5a expression in the regeneration of the inferior segment of rat hair follicle at embryonic and postnatal stages

doi:10.3969/j.issn.2095-4344.2136

Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (31): 5018-5022.doi: 10.3969/j.issn.2095-4344.2136

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Wnt5a expression in the regeneration of the inferior segment of rat hair follicle at embryonic and postnatal stages

Cai Bozhi1, Chen Xiancai2, Ni Na3, Lin Weishen4, Yuan Yanping5, Lin Changmin6, Huang Keng4

Received:2020-02-12 Revised:2020-02-21 Accepted:2020-03-24 Online:2020-11-08 Published:2020-09-04
Contact: Huang Keng, Chief physician, Master’s supervisor, Emergency Department of the Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong Province, China
About author:Cai Bozhi, Assistant experimentalist, Molecular Cardiology Laboratory of the First Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong Province, China
Supported by:
the National Natural Science Foundation of China No. 81972957 ; the Specific Scientific Development Foundation of Guangdong Province, No. 2017A030313776

Abstract

Abstract:

BACKGROUND: Wnt5a is the only signaling molecule that is highly expressed in the dermis and closely related to the occurrence of hair follicle dermal components.

OBJECTIVE: To preliminarily discuss initiation mechanism of dermal papilla embryogenesis and regeneration in vitro by observing Wnt5a expression of E12.5-E15.5 fetal rat skin and the upper part of tentacle hair follicle during dermal papilla regeneration induced by transplantation.

METHODS: The pregnant Sprague-Dawley rats at E12.5, E13.5, E14.5 and E15.5 days were killed under anesthesia. The embryos were taken out to make paraffin sections. The hairy papilla was removed from the tentacle hair follicles of Sprague-Dawley rats, and the hair follicles were removed from the upper segment of the hair follicles and then injected into the skin of the back of Nu/Nu nude mice. The skins of rat embryos at above gestational age and hair follicles at transplanted back positions of the nude mice were taken. Enzymatic immunohistochemical method was used to observe the Wnt5a expression in rat embryonic skin and the upper segment of hair follicle during dermal papilla regeneration induced by transplantation. Hematoxylin-eosin staining was used to observe the regeneration of the inferior segment of hair follicle after transplantation in the upper segment. The animal experiment was approved by the Animal Ethics Committee of Shantou University Medical College.

RESULTS AND CONCLUSION: (1) E12.5 Wnt5a was expressed in epidermal cells; E13.5 Wnt5a was expressed in epidermal cells and enhanced; E14.5 Wnt5a was transferred from epidermis to dermis and expressed in dermal cytoplasm; E15.5 Wnt5a was expressed in dermal connective tissue, obviously enhanced, and disappeared in epidermis. (2) From the third day to the ninth day after transplantation of the upper part of the rat hair follicle, the upper fragment of the hair follicle was observed to be growing downward. The complete follicles were re-formed. Wnt 5A was strongly expressed in the regeneration related tissues. (3) The high expression of Wnt5a in the site of dermal papilla formation in this model and dermal papilla embryogenesis suggests that Wnt signaling pathway is closely related to the regeneration of dermal papilla, and Wnt5a may play a very important role.

Key words: hair follicle, dermal papilla, dermis, embryo, pathway, mouse, experiment, regeneration

CLC Number:

Cai Bozhi, Chen Xiancai, Ni Na, Lin Weishen, Yuan Yanping, Lin Changmin, Huang Keng. Wnt5a expression in the regeneration of the inferior segment of rat hair follicle at embryonic and postnatal stages [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(31): 5018-5022.

Figures/Tables 3

References

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Wnt5a expression in the regeneration of the inferior segment of rat hair follicle at embryonic and postnatal stages

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