The performance research of a mixed material of collagen and clay

doi:10.3969/j.issn.2095-4344.2014.47.006

Abstract

Abstract:

BACKGROUND: Attapulgite clay is light with strong water absorption, and it has viscosity and plasticity in wet condition, which has been widely used in various fields. However, there is rarely report about attapulgite clay as bone repair material.
OBJECTIVE: To mix collagen with different proportion of attapulgite clay to fabricate a new type of tissue engineering scaffold material for bone repair.
METHODS: Collagen and attapulgite clay were melted by dry weight ratio of 1:0, 5:1, 4:1, 3:1 to fabricate scaffold materials. The apparent characteristics of the materials were observed by scanning electron microscopy, the porosity and water absorption of quality of materials were determined before and after 1% genipin cross-linked. Rat bone marrow mesenchymal stem cells were inoculated on the four different materials. The materials were observed by scanning electron microscopy after 3 days. And the materials were stained by hematoxylin-eosin staining after 7 days. The four different materials cross-linked by 1% genipin were implanted into rats and the degradation of materials was observed 1 month after implantation.
RESULTS AND CONCLUSION: Materials were sponge and porous. The porosity of materials was between 60% and 80% before cross-linked, while the porosity of materials was between 40% and 65% after cross-linked. There was no significant difference among four groups (P > 0.05). The water absorption of quality of the materials was significantly reduced after cross-linked (P < 0.05). The collagen-attapulgite clay scaffolds have good biocompatibility and can be completely degraded and absorbed in vivo without inflammation. The collagen-attapulgite clay materials of different ratios have different biodegradation rates.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: collagen, egg white, stem cells

CLC Number:

R318

Wang Jiu-na, Zhao Xing-xu, Tang Jun-jie, Li Gen, Jiang Tuo-zhi, Zhao Ling, Qin Wen, Zhao Hong-bin. The performance research of a mixed material of collagen and clay[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(47): 7573-7578.

Figures/Tables 5

References

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