Preparation and performance of biologic antimicrobial materials for pelvic tissue repair

doi:10.3969/j.issn.2095-4344.2014.43.015

Abstract

Abstract:

BACKGROUND: The biological extracellular matrix materials become the focus of pelvic floor repair materials research because of its excellent biocompatibility and mechanical compatibility. However, bacterial infection can damage the function of biological repair materials.
OBJECTIVE: To prepare the biologic antimicrobial materials for pelvic tissue function repair.
METHODS: Chitosan and tigecycline-loaded chitosan nanoparticles suspension was prepared by the electrostatic adsorption and self-aggregation of nanoparticles preparation techniques. Then the suspension was coated onto the surface of extracellular matrix materials. The morphological examination was performed by scanning electron microscope. The antibacterial property was detected by solid plate microbial culture method.
RESULTS AND CONCLUSION: Infrared spectra displayed that aromatic ring skeleton vibration peak of chitosan occurred on the biologic antimicrobial materials, and it was significantly widened at about 3 359 cm-1, indicating the composite coating was successful to modify the surface of extracellular matrix materials. As a very small dose
of tigecycline, there was no characteristic absorption peak on the infrared spectra. Scanning electron microscopy showed the porous structure of the material surface with some nanoparticles adhesion. The prepared materials had good antibacterial properties on Staphylococcus aureus and Escherichia coli, and the inhibition zone diameter was significantly increased with the increasing concentration of antibacterial agent. The biologic antimicrobial materials for pelvic tissue function repair were prepared successfully.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: nanoparticles, chitosan, pelvic floor

CLC Number:

R318

Ling You, Xu Bin, Chen Xiao-feng. Preparation and performance of biologic antimicrobial materials for pelvic tissue repair[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(43): 6979-6984.

Figures/Tables 2

2.1 生物型抗菌盆底修复材料的红外光谱检测红外光谱图1中A、B、C曲线分别为细胞外基质材料、壳聚糖复合细胞外基质材料及生物型抗菌修复材料。由图可见，相对细胞外基质材料而言，复合壳聚糖后在3 359 cm-1左右峰明显增宽，归属于O-H伸缩振动吸收峰与N-H的伸缩振动吸收峰，是壳聚糖复合后分子内和分子间氢键缔合重叠而产生的多重特征吸收峰。1 647 cm-1为C=O伸缩振动吸收峰，1 559 cm-1为N-H的面内弯曲振动吸收峰，1 378 cm-1为N-H的面外弯曲振动吸收峰[23]。 1 152 cm-1为C-O-C氧桥的不对称伸缩振动峰，1 068 cm-1和1 028 cm-1为糖环骨架的伸缩振动峰，峰吸收强，是复合壳聚糖后特征吸收，而细胞外基质材料吸收谱中未见该吸收，说明壳聚糖在细胞外基质材料上复合成功[24-27]。 2.2 生物型抗菌盆底修复材料的表面形貌图2A，B和图2C，D分别为细胞外基质材料和生物型抗菌修复材料的微观表面形貌图。由图可见，细胞外基质材料主要由多层排列有序的纤维丝组成，各层有序排列的纤维丝取向各异，由此形成三维空间网架多孔结构(图2A)，纤维丝表面光滑，无明显其他物质附着在纤维丝表面(图2B)。生物型抗菌修复材料同样保存了多层排列有序的纤维丝构成的主体结构(图2C)，并且在微丝表面和微丝之间还明显附着一层蓬松鳞片状涂层物质(图2D)，材料微表面三维孔洞变小，但仍维持多孔连通特性。 2.3 生物型抗菌盆底修复材料的抗菌效果如图3所示，对大肠埃希菌和金黄色葡萄球菌培养24 h后，细胞外基质材料组平板内菌胎生长正常，无抑菌圈出现，均未表现出明显的抗菌性能。生物型抗菌盆底修复材料组在25，50，100 g/L的抗菌剂量时均出现明显抑菌圈，并且随着抗菌剂质量浓度的增大，抑菌圈直径显著增加，表现出良好的抗菌性能。为考察生物型盆底抗菌材料持续抗菌能力，实验含菌平板继续培养至4 d，每天测量抑菌圈大小，见表1所示，尽管随着时间的延长，平板内菌胎长得很密，各抗菌剂量的生物型盆底修复材料抑菌圈直径仅有略微缩小，未见明显变化。第4天与第1天相比，同一抗菌剂量组大肠埃希菌和金黄色葡萄球菌两种受试菌株平板抑菌圈大小差异均无显著性意义。"

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