Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (43): 6959-6965.doi: 10.3969/j.issn.2095-4344.2014.43.012

Previous Articles     Next Articles

Variation of bone morphogenetic protein-2 and vascular endothelial growth factor after bone defect filled with tricalcium phosphate

Xu Jun, Yin Xiao-fan, Gu Hui-jie, Qin Qiang   

  1. Department of Orthopedics, Shanghai Minhang District Central Hospital, Shanghai 201199, China
  • Received:2014-09-13 Online:2014-10-15 Published:2014-10-15
  • Contact: Yin Xiao-fan, Chief physician, Department of Orthopedics, Shanghai Minhang District Central Hospital, Shanghai 201199, China
  • About author:Xu Jun, Master, Attending physician, Department of Orthopedics, Shanghai Minhang District Central Hospital, Shanghai 201199, China
  • Supported by:

    the Natural Science Research Project of Minhang District of Shanghai, No. 2013MHZ002

PDF

373

Abstract:

BACKGROUND: Currently, bone graft is mainly used for repair of bone defects, and tricalcium phosphate is the most used artificial bone material. But the effectiveness of the tricalcium phosphate bone graft is still controversial, and there is also no detailed report about its function during the healing of bone defect.
OBJECTIVE: To observe the concentration changes of bone morphogenetic protein-2 and vascular endothelial growth factor as well as bone healing after tricalcium phosphate graft in bone defects.
METHODS: Forty-eight C57 mice were randomly divided to experimental group and control group. A 2-mm-long diaphyseal segment and periosteum from the middle of the right femur was cut to prepare unilateral bone defect models. Tricalcium phosphate bone graft was used in the experimental group, and no bone graft in the control group. During the following 4 weeks, X-ray examination was done once a week to observe the bone healing, and then the animals were executed for collecting samples in the graft area. The concentrations of bone morphogenetic protein-2 and vascular endothelial growth factor in samples which were taken from the bone graft area were determined by using ELISA assay.
RESULTS AND CONCLUSION: X-ray films showed that 2 weeks later, bone fracture healed mostly in the  experimental group except a small part of cortical bone; 3 weeks later, bone fracture was basically healed, and only a small amount of tricalcium phosphate remained; 4 weeks later, bone fracture was completely healed, and the callus grew obviously, and the tricalcium phosphate was nearly absorbed. In the control group, the fracture line was still visible at 1-2 weeks, but it became vague at 3 weeks; then, the fracture was healed at 4 weeks except some of the cortical bone. The levels of bone morphogenetic protein-2 and vascular endothelial growth factor were significantly higher in the experimental group than in the control group at different time points (P < 0.05). These results suggest that tricalcium phosphate bone graft can up-regulate the expression of bone morphogenetic protein-2 and vascular endothelial growth factor and accelerate bone healing.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: calcium phosphates, bone morphogenetic proteins, vascular endothelial growth factors

CLC Number: