Effect of Bio-Gide collagen membranes on the proliferation and osteogenetic differentiation of rabbit bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.30.007

Abstract

Abstract:

BACKGROUND: Bio-Gide collagen membranes show a good biocompatibility with stem cells. But the research on the osteogenetic differentiation of bone marrow mesenchymal stem cells cultured on the Bio-Gide collagen membranes is rarely reported.
OBJECTIVE: To observe the effect of Bio-Gide collagen membranes on the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells.
METHODS: Bone marrow mesenchymal stem cells from rabbits were isolated and cultured by using the whole bone marrow adherence method in vitro. Passage 3 bone marrow mesenchymal stem cells were selected and seeded on the Bio-Gide collagen membrane pretreated petri dish (experimental group) and simple petri dish (control group). The proliferation of bone marrow mesenchymal stem cells was detected by Cell Counting Kit-8 at 1, 4, 7, 14 days. The supernatant of the cells cultured in osteogenic differentiation medium were collected to detect the activity of alkaline phosphatase at 1, 4, 7, 14 days.
RESULTS AND CONCLUSION: The number of bone marrow mesenchymal stem cells in the two groups was increased with the increasing time, and the control group had more cells than the experimental group at 7 days (P < 0.05). There was no significant difference between the two groups at other time points. The alkaline phosphatase activity was increased with the increasing culture time, and the experimental group had a higher activity than the control group at 14 days (P < 0.05). There was no significant difference between the two groups at other time points. Experimental findings indicate that Bio-Gide collagen membranes can promote the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: stem cells, collagen, cell differentiation, bone regeneration

CLC Number:

R318

Xu Fan, Yang De-sheng. Effect of Bio-Gide collagen membranes on the proliferation and osteogenetic differentiation of rabbit bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(30): 4797-4803.

Figures/Tables 6

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