Three-dimensional growth of adipose-derived stem cells in bone tissue engineering complex

doi:10.3969/j.issn.2095-4344.2014.24.005

Abstract

Abstract:

BACKGROUND: Tissue engineering scaffolds are designed to imitate extracellular matrix, which is essential for cellular growth and metabolism. The scaffold preparation and the selection of seed cells are the hot topics in bone tissue engineering field.
OBJECTIVE: To construct three-dimensional growth environment of adipose-derived stem cells with the chitosan/xenogeneic bone, and investigate its compatibility. METHODS: Adipose tissue was obtained from New Zealand white rabbits at 8 days old, and adipose-derived stem cells were extracted. After induction of osteogenic differentiation in vitro, the cells were cultured on chitosan/xenogenic bone, to construct bone tissue engineering complex, serving as the experimental group. The adipose-derived stem cells were directly cultured in xenogenic bone, to construct cells/xenogeneic bone complex, serving as a control group. The simple bone xenograft served as a blank group. Two weeks after in vitro induced differentiation, the complex or xenograft in three groups were observed under scanning electron microscope to observe the cells and scaffold compound the situation.
RESULTS AND CONCLUSION: The chitosan fully penetrated into the scaffolds, and formed a three-dimensional environment for cell growth, so that adipose-derived stem cells can grow in the three-dimensional environment, which provide sufficient space for the regeneration of the extracellular matrix. After induction of the chitosan/xenogenic bone, adipose-derived stem cells can carry more cells, and the loss of cells in the vector is reduced, so it is a good carrier of bone tissue engineering.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: stem cells, subcutaneous fat, gels, braces, tissue engineering

CLC Number:

R318

Wang Jian-wu, Dang Jian-jun, Li Qiang, Li Wen-gao, Zheng Yu. Three-dimensional growth of adipose-derived stem cells in bone tissue engineering complex[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(24): 3797-3802.

Figures/Tables 5

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