Chondrogenic differentiation of rat synovial-derived mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.15.008

Abstract

Abstract:

BACKGROUND: Compared with other sources of mesenchymal stem cells, synovial-derived mesenchymal stem cells have significant characteristics of chondrogenesis and cloning. Therefore, synovial-derived mesenchymal stem cells are one of the most promising seed cells in cartilage tissue engineering.
OBJECTIVE: To isolate and culture synovial-derived mesenchymal stem cells of Sprague-Dawley rats, identify the multipotential differentiation and the potential ability of chondrogenic differentiation in three-dimensional culture condition.
METHODS: The synovium tissue was harvested from Sprague-Dawley rats. The synovial-derived mesenchymal stem cells were isolated with type Ⅰ collagen enzyme digestion method and cultured in vitro. The passage 3 cells were detected with giemsa staining, the cell cycle, adipogenic and osteogenic differentiation were determined. The passage 3 cells were centrifuged as pellets and cultured in the chondriogenic medium for 21 days. And the pellets were examined by toluidine blue staining, type Ⅱ collagen immunohistochemical staining and RT-PCR.
RESULTS AND CONCLUSION: The mesenchymal stem cells isolated from the synovium tissue of rats have the characteristics of mesenchymal stem cells, and exhibit fibroblast-like morphology after cultured in vitro. The multilineage differentiation potentials were also revealed. After the cell were cultured in chondrogenic medium for 21 days, chondroid tissue was found, type II collagen and aggrecan could be detected positively by toluidine blue staining, type Ⅱ collagen immunohistochemical staining, and expressed by RT-PCR examination. Therefore, synovial mesenchymal stem cells have a chondrogenic differentiation potential.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, collagen type II, reverse transcriptase polymerase chain reaction

CLC Number:

R318

Shao Bo, Gong Zhong-cheng, Liu Hui, Ling Bin, Keremu Abass, Yin Xiao-peng, Hu Lu-lu, Wang Bing, Ning Xiao-ting, Yang Meng, Lin Zhao-quan. Chondrogenic differentiation of rat synovial-derived mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(15): 2338-2344.

Figures/Tables 4

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