Hypoxia-inducible factor-1 alpha effects on bone marrow mesenchymal stem cell mobilization in rats with acute myocardial infarction

doi:10.3969/j.issn.2095-4344.2014.10.016

Abstract

Abstract:

BACKGROUND: Increasing autologous stem cell mobilization is conceived to achieve effectively repair of cardiac ischemic injury. Therefore, it is important to seek a specific and effective mobilization agent.
OBJECTIVE: To observe the effects of hypoxia-inducible factor-1α (HIF-1α) on bone marrow mesenchymal stem cell mobilization in myocardial infarction.
METHODS: Left anterior descending artery was ligated to establish a rat model of acute myocardial infarction in 90 outbreeding Sprague-Dawley rats, and then the models were randomly divided into three groups. In HIF-1α-antisense oligonucleotide (ASODN) group, HIF-1α-ASODN was infused into the tail vein to restrain the expression of HIF-1α in infarcted ischemic tissue. In HIF-1α-missense oligonucleotide (MSODN) group or control group, an equal volume of HIF-1α-MSODN or saline was injected.
RESULTS AND CONCLUSION: After 30 hours and 7 days of modeling, the number of bone marrow mesenchymal stem cells and expression of vascular endothelial growth factor in the peripheral blood of the control group were similar to the HIF-1α-MSODN group, but significantly higher than the HIF-1α-ASODN group.
After 7 days of modeling, the expressions of HIF-1α protein, vascular endothelial growth factor protein and mRNA in the ischemic myocardial tissues of the control group were similar to the HIF-1α-MSODN group, but significantly higher than the HIF-1α-ASODN group. After 7, 14 and 28 days of modeling, the capillary density in the ischemic myocardial tissues of the control group was similar to the HIF-1α-MSODN group, but significantly higher than the HIF-1α-ASODN group. These findings indicate that after acute myocardial infarction, high expression of HIF-1α exhibits a causal relationship with mobilization of bone marrow mesenchymal stem cells, initiating a series of self-healing process of myocardial tissues.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, hypoxia-inducible factor 1, alpha subunit, myocardial infarction

CLC Number:

R394.2

Qi Jin-wei, Cheng Jing-lin, Zhou Shu, Li Jing-rong, Li Xue-xiang, Yang Qin, Zhang Hao, Wan Jun, Wang Yu-lin, Zhang Li-xin, Chen Yun-yun, Xi Xiu-xia, Ye Li, Tang Qian, Xu Feng, Jang Yang. Hypoxia-inducible factor-1 alpha effects on bone marrow mesenchymal stem cell mobilization in rats with acute myocardial infarction[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(10): 1579-1584.

Figures/Tables 4

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