Effect of basic fibroblast growth factor on the migration of human adipose-derived stem cells toward vascular endothelium

doi:10.3969/j.issn.2095-4344.2014.10.015

Abstract

Abstract:

BACKGROUND: The establishment of a good blood supply is a key mechanism for successful implantation of engineered tissues.
OBJECTIVE: To observe the effect of basic fibroblast growth factor on the migration of human adipose-derived stem cells via implanting the human adipose-derived stem cells and sodium hyaluronate composite graft at the subcutaneous site of BALB/C mice, in order to explore an optimal scheme for soft tissue reconstruction.
METHODS: Human adipose-derived stem cells were isolated from the adipose tissue of healthy cosmetic patients which received liposuction, and the cells were subcultured. Then 5×10⁹/L passage 3 cell suspension labeled by cm-dil was prepared. The working solution containing 2 mg/L basic fibroblast growth factor was prepared. Composite tissue al-lografts which were the mixtures of 0.25 mL sodium hyaluronate, 0.2 mL cell suspension and 0.05 mL working solution or DMEM were implanted into the subcutaneous site of both sides of the mouse back. Specimens were taken at 6 weeks after operation and were evaluated histologically after hematoxylin-eosin and vascular immunofluorescent staining.
RESULTS AND CONCLUSION: No necrosis, liquefaction, nodular tissue or gel remained in operated position. The hematoxylin-eosin staining showed the main components of the specimens were the adipose tissue and the
loose connective tissue. The immunofluorescence staining showed the overlaps between the cm-dil fluorescence from human adipose-derived stem cells and the FITC fluorescence from the vascular endothelium in the experimental group were more than those in the control group (P < 0.05). Basic fibroblast growth factor promotes the migration and the differentiation of human adipose-derived stem cells in the sodium hyaluronate scaffold into vascular endothelium.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, subcutaneous fat, adipose tissue, fibroblast growth factors, endothelium, vascular, hyaluronic acid

CLC Number:

R394.2

Zhu Meng-lin, Jiang Nan, Xu Yang-yang, Cao Jing, Yang Liu. Effect of basic fibroblast growth factor on the migration of human adipose-derived stem cells toward vascular endothelium[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(10): 1573-1578.

Figures/Tables 6

References

[1]Morrison WA. Progress in tissueengineering of soft tissue and organs. Surgery. 2009;145(2):127-130.
[2]Jaklenec A, Stamp A, Deweerd E, et al. Progress in the tissue engineering and stem cell industry "are we there yet?". Tissue Eng Part B Rev. 2012;18(3):155-166.
[3]Martino S, D'Angelo F, Armentano I, et al. Stem cell-biomaterial interactions for regenerative medicine. Biotechnol Adv. 2012;30(1):338-351.
[4]Bhat S, Kumar A. Cell proliferation on three-dimensional chitosan-agarose-gelatin cryogel scaffolds for tissue engineering applications. J Biosci Bioeng. 2012;114(6):663-670.
[5]Mahmoudifar N, Doran PM. Osteogenic differentiation and osteochondral tissue engineering using human adipose- derived stem cells. Biotechnol Prog. 2013;29(1):76-185.
[6]Wang W, Cao B, Cui L, et al. Adipose tissue engineering with human adipose tissue-derived adult stem cells and a novel porous scaffold. J Biomed Mater Res B Appl Biomater. 2013; 101(1):68-75.
[7]Declercq HA, De Caluwé T, Krysko O, et al. Bone grafts engineered from human adipose-derived stem cells in dynamic 3D-environments. Biomaterials. 2013;34(4): 1004-1017.
[8]Kwan MD, Sellmyer MA, Quarto N, et al. Chemical control of FGF-2 release for promoting calvarial healing with adipose stem cells. J Biol Chem. 2011;286(13):11307-11313.
[9]Kang JM, Han M, Park IS, et al. Adhesion and differentiation of adipose-derived stem cells on a substrate with immobilized fibroblast growth factor. Acta Biomater. 2012;8(5):1759-1767.
[10]Kotulák T, Drápalová J, Kopecký P, et al. Increased circulating and epicardial adipose tissue mRNA expression of fibroblast growth factor-21 after cardiacsurgery: possible role in postoperative inflammatory response and insulin resistance. Physiol Res. 2011;60(5):757-767.
[11]徐扬阳,姜南,杨柳,等.重组人碱性成纤维细胞生长因子对人脂肪干细胞分化为脂肪细胞的影响[J].中华医学美学美容杂志,2013, 19(2):134-137.
[12]Von Heimburg D, Hemmrich K,Zachariah S,et al. Oxygen consumption in undifferentiated versus differentiated adipogenic mesenchymal precursor cells. Respir Physiol Neurobiol. 2005;146(2-3):107-116.
[13]Beahm EK,Walton RL,Patrick Jr CW. Progress in adipose tissue construct development. Clin Plast Surg. 2003;30(4): 547-558.
[14]刘琳,张亚,周云,等.脂肪源干细胞向血管内皮细胞的分化[J].中国组织工程研究,2013,17(23):4224-4231.
[15]Strem BM, Hedrick MH. The growing importance of fat in regenerative medicine. Trends Biotechnol. 2005;23(2):64-66.
[16]Strem BM, Hicok KC, Zhu M, et al. Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med. 2005;54(3):132-141.
[17]Handel M1, Hammer TR, Hoefer D. Adipogenic differentiation of scaffold-bound human adipose tissue-derived stem cells (hASC) for soft tissue engineering. Biomed Mater. 2012;7(5): 054107.
[18]Matsumoto D, Sato K, Gonda K, et al. Cell-assisted lipotransfer: supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection. Tissue Eng. 2006;12(12):3375-3382.
[19]Neofytou EA, Chang E, Patlola B, et al. Adipose tissue-derived stem cells display a proangiogenic phenotype on 3D scaffolds. J Biomed Mater Res A. 2011; 98(3):383-393.
[20]Hemmrich K, Van de Sijpe K, Rhodes NP, et al. Autologous in vivo adipose tissue engineering in hyaluronan-based gels--A pilot study. J Surg Res. 2008;144(1):82-88.
[21]Eppley BL, Sidner RA, PIatis JM, et al. Bioactivation of free-fat transfers:a potential new approach to improving graft survival. Plast Reconstr Surg. 1992;90(6):1022-1030.
[22]Tabata Y, Miyao M, Inamoto T,et al. De novo formation of adipose tissue by controlled release of basic fibroblast growth factor. Tissue Eng. 2000;6(3):279-289.
[23]黎洪棉,高建华,吴涛,等. 脂肪干细胞与外源性血管内皮生长因子及纤维蛋白胶复合物体内构建血管化组织工程脂肪[J].中国组织工程研究与临床康复,2011,15(40):7480-7484.