Effects of human bone marrow cells-derived extracellular matrix on the proliferation of human periodontal ligament stem cells

doi:10.3969/j.issn.2095-4344.2014.06.019

Abstract

Abstract:

BACKGROUND: Human bone marrow cells-derived extracellular matrix can promote proliferation of human periodontal ligament stem cells and maintain stem cell properties.

OBJECTIVE: To preliminarily investigate the effect of human bone marrow cells-derived extracellular matrix on the proliferation of human periodontal ligament stem cells.

METHODS: Human periodontal ligament stem cells and bone marrow cells were separately derived from human periodontal tissue and jaw bone marrow, and human bone marrow cells-derived extracellular matrix was prepared. Human periodontal ligament stem cells were cultured and purified using limited dilution cloning method, and transmission electron microscope was used for ultrastructure observation. Human periodontal ligament stem cells at passage 2 were cultured with human bone marrow cells-derived extracellular matrix and normal culture medium (control group). The cell counting kit-8 and flow cytometry were used to determine the proliferation potential of human periodontal ligament stem cells cultured on human bone marrow cells-derived extracellular matrix.

RESULTS AND CONCLUSION: Compared with the control group, human periodontal ligament stem cells cultured on human bone marrow cells-derived extracellular matrix had a superior capacity of proliferation (P < 0.05), and the cells met their morphological and biological characteristics, and grew in good conditions. Human bone marrow cells-derived extracellular matrix is a promising matrix for large-scale expanding human periodontal ligament stem cells for future use in stem cell-based therapy.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, extracellular matrix, periodontal ligament

CLC Number:

R394.2

Wang A-xian, Tang Li, Liang Yuan, Ji Hai-ning, Wu Jun-jie, Ding Yin. Effects of human bone marrow cells-derived extracellular matrix on the proliferation of human periodontal ligament stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(6): 938-943.

Figures/Tables 2

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