Chondrocyte supernatant induces chondrogenesis and pellet cultivation of rat synovial mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.01.017

Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (1): 100-105.doi: 10.3969/j.issn.2095-4344.2014.01.017

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Chondrocyte supernatant induces chondrogenesis and pellet cultivation of rat synovial mesenchymal stem cells

Shao Bo, Gong Zhong-cheng, Liu Hui, Ling Bin, Ke Re-mu, Yin Xiao-peng, Hu Lu-lu, Wang Bing, Ning Xiao-ting, Lin Zhao-quan

Oncology Department of Oral & Maxillofacial Surgery, the First Teaching Hospital of Xinjiang Medical University, Stomatology College of Xinjiang Medical University, Stomatology Research Institute of Xinjiang Province, Urumqi 830054, Xinjiang Uygur Autonomous Region, China

Revised:2013-12-10 Online:2014-01-01 Published:2014-01-01
Contact: Gong Zhong-cheng, Oncology Department of Oral & Maxillofacial Surgery, the First Teaching Hospital of Xinjiang Medical University, Stomatology College of Xinjiang Medical University, Stomatology Research Institute of Xinjiang Province, Urumqi 830054, Xinjiang Uygur Autonomous Region, China Corresponding author: Lin Zhao-quan, Chief physician, Oncology Department of Oral & Maxillofacial Surgery, the First Teaching Hospital of Xinjiang Medical University, Stomatology College of Xinjiang Medical University, Stomatology Research Institute of Xinjiang Province, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
About author:Shao Bo, Studying for master’s degree, Oncology Department of Oral & Maxillofacial Surgery, the First Teaching Hospital of Xinjiang Medical University, Stomatology College of Xinjiang Medical University, Stomatology Research Institute of Xinjiang Province, Urumqi 830054, Xinjiang Uygur Autonomous Region, China Gong Zhong-cheng, M.D., Associate chief physician, Oncology Department of Oral & Maxillofacial Surgery, the First Teaching Hospital of Xinjiang Medical University, Stomatology College of Xinjiang Medical University, Stomatology Research Institute of Xinjiang Province, Urumqi 830054, Xinjiang Uygur Autonomous Region, China Shao Bo and Gong Zhong-cheng contributed equally to this work.
Supported by:
the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2011211A068

Abstract

Abstract:

BACKGROUND: Synovial mesenchymal stem cells have the ability of multilineage differentiation in vitro, which are expected to be seed cells for the treatment of cartilage defects in cartilage tissue engineering. Appropriate growth factors are critical for the chondrocyte differentiation of synovial mesenchymal stem cells.
OBJECTIVE: To study the role of secreted factors by chondrocytes to induce chondrogenesis of synovial mesenchymal stem cells.
METHODS: The synovial mesenchymal stem cells and chondrocytes were harvested from rat knee joints and cultured through the digestion method. The supernatant was collected from chondrocytes, and centrifuged, filtered and cryopreserved. The third passage synovial mesenchymal stem cells centrifuged as pellets were cultured in the chondrocyte supernatant for 21 days. And the cells morphology was examined and the type II collagen and aggrecan were detected through immunohistochemistry and RT-PCR.
RESULTS AND CONCLUSION: The synovial mesenchymal stem cell pellets cultured in the chondrocyte supernatant became cartilage-like tissue after 21 days. The type II collagen was detected positively in the matrix of synovial mesenchymal stem cell pellet immunohistochemically. RT-PCR examination showed that the type II collagen and aggrecan expressed in the synovial mesenchymal stem cellpellet cultured in the chondrocyte supernatant. It suggested that synovial mesenchymal stem cell could be induced to differentiate into chondrocytes depending on soluble factors secreted by chondrocytes.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: chondrocytes, synovial membrane, mesenchymal stem cells, cell culture, cell differentiation, tissue engineering

CLC Number:

R394.2

Shao Bo, Gong Zhong-cheng, Liu Hui, Ling Bin, Ke Re-mu, Yin Xiao-peng, Hu Lu-lu, Wang Bing, Ning Xiao-ting, Lin Zhao-quan. Chondrocyte supernatant induces chondrogenesis and pellet cultivation of rat synovial mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(1): 100-105.

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Chondrocyte supernatant induces chondrogenesis and pellet cultivation of rat synovial mesenchymal stem cells

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