Effect of hypoxia and serum deprivation on endogenous hydrogen sulfide production in rat bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.01.003

Abstract

Abstract:

BACKGROUND: Ischemia microenvironment contributes mostly to the low survival rate of rat bone marrow mesenchymal stem cells after transplantation. Hydrogen sulfide (H2S) can protect various cells and tissue models against apoptosis and injury.
OBJECTIVE: To detect the cell apoptosis and viability, content of H2S in supernatant, and the expression of H2S synthetase after different time of hypoxia and serum deprivation cultivation of rat bone marrow mesenchymal stem cells.
METHODS: The passage 3 rat bone marrow mesenchymal stem cells were divided into five different cultivation time groups: 0-, 3-, 6-, 12- and 24-hour groups. After enough hypoxia and serum deprivation cultivated time, the cell apoptosis was detected by SubG1, the cell viability was determined by cell counting kit-8, the content of H2S in supernatant was measured by N,N-dimethyl-p-phenylenediamin and the expression of H2S synthetase by RT-PCR and western blot.
RESULTS AND CONCLUSION: Compared to the normal cultivation group, after different hypoxia and serum
deprivation cultivated time, the cell apoptosis increased and cell viability decreased significantly. The longer hypoxia and serum deprivation cultivated time caused the more cell apoptosis and the lower cell viability. The contents of H2S and its synthetase were also suppressed by hypoxia and serum deprivation cultivation. The difference was statistically significant. These findings suggest that hypoxia and serum deprivation cultivation can inhibit the generation of H2S and expression of its synthetase.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, bone marrow, mesenchymal stem cells, anoxia, culture media, serum-free, hydrogen sulfide, apoptosis

CLC Number:

R394.2

Guo Zeng, Li Cong-sheng, Xie Yang-jing, Wang Chun-miao, Cheng Jing-lin, Wang Ai-ling . Effect of hypoxia and serum deprivation on endogenous hydrogen sulfide production in rat bone marrow mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(1): 14-20.

Figures/Tables 5

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