Anti-aging action of human umbilical cord mesenchymal stem cells combined with lycopene

doi:10.3969/j.issn.2095-4344.2013.40.004

Abstract

Abstract:

BACKGROUND: Studies have demonstrated the anti-aging action of human umbilical cord mesenchymal stem cells or lycopene. However, there are few researches on the anti-aging action of their combination.
OBJECTIVE: To investigate the anti-aging effects of human umbilical cord mesenchymal stem cells combined with lycopene on D-galactose-induced aging model in mice.
METHODS: Subactute aging model was established in ICR mice by subcutaneous injection of D-galactose. The experimental mice were divided into control group, model group, stem cell group, lycopene and stem cells+lycopene group. The mice were sacrificed after 8 weeks. The activity of superoxide dismutase, the content of malondialdehyde in serum, liver, brain and the content of hydroxyproline in skin were determined.
RESULTS AND CONCLUSION: Compared with the control group, the activity of superoxide dismutase and the content of hydroxyproline were significantly reduced, but the content of malondialdehyde significantly increased in model group mice (P ≤ 0.01). Compared with three treatment groups, the activity of superoxide dismutase and the content of hydroxyproline were significantly lower, but the content of malondialdehyde significantly greater in the model group (P ≤ 0.05). However, there was no statistical significance in content of malondialdehyde between model and lycopene groups (P > 0.05). The activity of superoxide dismutase and the content of hydroxyproline were significantly higher, but the content of malondialdehyde significantly lower in stem cells+lycopene group compared with stem cell or lycopene groups (P ≤ 0.05). Results suggested that human umbilical cord mesenchymal stem cells and lycopene have anti-aging effects, and the anti-aging effects of the combination of them are better than either of them.

Key words: stem cells, umbilical cord, sirtuins, superoxide dismutase, malondialdehyde

CLC Number:

R394.2

Li Qian-qian, Cui Xiao-lan, Shi Han, Liu Jia, Cheng Xue, Wang Yi-zhong. Anti-aging action of human umbilical cord mesenchymal stem cells combined with lycopene[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(40): 7040-7046.

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