Preparation and performance evaluation of calcium sodium carboxymethyl cellulose/ hydroxypropyltrimethyl ammonium chloride chitosan multilayer dressing

doi:10.12307/2023.171

Abstract

Abstract: BACKGROUND: Moist dressings have been widely accepted, but they usually lack antibacterial properties, and their hemostatic activity and moisture-retention can be further improved.
OBJECTIVE: To construct calcium sodium carboxymethyl cellulose/hydroxypropyltrimethyl ammonium chloride chitosan multilayer dressing, and to investigate its physical-chemical properties and evaluate antibacterial, hemostatic and biocompatible performances.
METHODS: Calcium sodium carboxymethyl cellulose was prepared by ion exchange method, and calcium sodium carboxymethyl cellulose / hydroxypropyltrimethyl ammonium chloride chitosan multilayer dressing (calcium sodium carboxymethyl cellulose:hydroxypropyltrimethyl ammonium chloride chitosan=2:1, 1:1, 1:2) was prepared based on interfacial polyelectrolyte complexation. The water absorption and water vapor transmission of the multilayer films were determined. Fourier transform infrared spectrometry, scanning electron microscopy and energy dispersive spectroscopy were used to characterize the composition, morphology and structure of the multilayer films. The hemostatic performance, antibacterial activity and cytocompatibility of the multilayer films were evaluated by whole blood clotting, plate counting count and co-culture method, respectively.
RESULTS AND CONCLUSION: (1) Fourier transform infrared spectrometry and energy dispersive spectroscopy revealed that multilayer film dressing was successfully constructed. Scanning electron microscopy exhibited that the multilayer film was composed of calcium sodium carboxymethyl cellulose layer, hydroxypropyltrimethyl ammonium chloride chitosan layer and polyelectrolyte complex interface layer, which was formed by interfacial complexation. (2) The three multilayer films absorbed water rapidly in the first 10 minutes, and then the water absorption rate gradually slowed down and showed a stable trend. Compared with the dissolution tendency of pure calcium sodium carboxymethyl cellulose film, the multilayer film could be stable for a long time without collapsing after absorbing water in PBS. The water vapor transmission rates of the three multilayer films were lower than those of pure calcium sodium carboxymethyl cellulose films. (3) Pure calcium sodium carboxymethyl cellulose film had no antibacterial properties. The multilayer film had antibacterial properties. With the increase of the proportion of hydroxypropyltrimethyl ammonium chloride chitosan in the multilayer films, the antibacterial rate against Staphylococcus aureus and Escherichia coli increased. (4) The procoagulant property of the multilayer film (1:2) was weaker than that of the multilayer film (2:1, 1:1) and the pure calcium sodium carboxymethyl cellulose film. (5) CCK-8 assay showed that the proliferation rate of fibroblasts on the multilayer film (1:2) was lower than that on the multilayer film (2:1, 1:1) and pure calcium sodium carboxymethyl cellulose film (P < 0.05). Under the laser confocal microscope, the fibroblasts on the membranes of each group grew well and were spindle or polygonal. (6) The results show that the calcium sodium carboxymethyl cellulose/hydroxypropyltrimethyl ammonium chloride chitosan multilayer film has good water absorption, moisturizing, hemostatic and antibacterial properties.

Key words: carboxymethyl cellulose, hydroxypropyltrimethyl ammonium chloride chitosan, multilayer film, moist dressing, antibacterial, calcium ion, interfacial polyelectrolyte complexation, hemostasis, cytocompatibility

CLC Number:

Pan Wei, Li Shuyang, Liu Jinhui, Liang Cheng, Duan Ke, Chen Xingtao. Preparation and performance evaluation of calcium sodium carboxymethyl cellulose/ hydroxypropyltrimethyl ammonium chloride chitosan multilayer dressing[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(25): 3949-3955.

Figures/Tables 7

References

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