Aligned poly(butylene adipate-co-terephthalate)/type I collagen fibers promote tendon-bone healing after anterior cruciate ligament rupture

doi:10.12307/2022.861

Abstract

Abstract: BACKGROUND: The micro/nano structure on the surface of the material has a regulatory effect on the behavior of the cells. The tendon tissue is mainly composed of parallel collagen fibers, so the aligned fiber structure has a certain promotion effect on tendon-bone healing.
OBJECTIVE: To explore the biocompatibility and osteoinductive activity of poly(butylene adipate-co-terephthalate) (PBAT)/type I collagen aligned fiber scaffolds.
METHODS: Electrospinning technology was used to prepare random PBAT, aligned PBAT, and aligned PBAT/type I collagen fiber scaffold materials. The static contact angle and mechanical properties of the three stents were characterized. The three scaffolds were co-cultured with rat bone marrow mesenchymal stem cells to detect cell adhesion rate and proliferation. DAPI staining and Live/Dead fluorescent staining were used to observe cell adhesion and survival on the scaffold. Alkaline phosphatase staining and type I collagen immunohistochemical staining were used to detect the osteoinductive activity of oriented fiber scaffolds.
RESULTS AND CONCLUSION: (1) The static contact angle of oriented PBAT/type I collagen fiber scaffold was lower than that of random PBAT and aligned PBAT fiber scaffolds (P < 0.05). (2) The strain degree of the two aligned scaffolds was better than that of the aligned PBAT scaffold (P < 0.05), and the tensile strength of the aligned PBAT scaffold was better than that of the random PBAT group (P < 0.05). There was no significant difference in the elastic modulus of the three groups of scaffolds (P > 0.05). (3) The cell adhesion and proliferation on the aligned PBAT/type I collagen fiber scaffold were better than those of the other two scaffolds. (4) DAPI staining and Live/Dead fluorescent staining showed that bone marrow mesenchymal stem cells could adhere and proliferate on the three groups of scaffolds, and the number of cells on the aligned PBAT/type I collagen fiber scaffold was the largest. (5) Alkaline phosphatase staining and type I collagen immunohistochemical staining showed that the osteoinductive activity of the aligned PBAT/type I collagen fiber scaffold was stronger than that of the aligned PBAT fiber scaffold. (6) The results show that the PBAT/type I collagen aligned fiber scaffold has good biocompatibility and osteoinductive activity and can effectively promote cell proliferation and adhesion.

Key words: poly(butylene adipate-co-terephthalate), type I collagen, tissue engineering, aligned fibers, stem cells, electrospinning, biomaterials

CLC Number:

Hu Qiuyu, Yang Long, Yang Yong, Song Shenchao. Aligned poly(butylene adipate-co-terephthalate)/type I collagen fibers promote tendon-bone healing after anterior cruciate ligament rupture[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(27): 4314-4319.

Figures/Tables 8

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