Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (22): 3445-3449.doi: 10.3969/j.issn.2095-4344.3182

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Effects of hemostasis and promoting wound healing of ozone sustained-release hydrogel

Liu Fang, Shan Zhengming, Tang Yulei, Wu Xiaomin, Tian Weiqun   

  1. Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, Hubei Province, China
  • Received:2020-02-13 Revised:2020-02-24 Accepted:2020-06-29 Online:2021-08-08 Published:2021-01-19
  • Contact: Tian Weiqun, MD, Master’s supervisor, Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, Hubei Province, China
  • About author:Liu Fang, Master candidate, Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, Hubei Province, China
  • Supported by:
    Innovation Experiment Program for Medical College Students of Wuhan University, No. MS2018006 (to TWQ)

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Abstract: BACKGROUND: Ozone has good bacteriostatic and hemostatic effects, but its poor stability and easy decomposition limit its application and promotion.
OBJECTIVE: To prepare ozone sustained-release hydrogel, and to evaluate its stability and hemostasis and promoting effect on wound healing.
METHODS: (1) With carbomer as the substrate, the ozone sustained-release hydrogels with ozone concentrations of (3.4±0.2)×10-6, (2.3±1.8)×10-6, (7.20±0.5)×10-6, (7.0 ±3.0)×10-6, (16.4±1.2)×10-6, and (15.0±5.0)×10-6 were prepared by controlling the ozone penetration time, and the stability of ozone in the ozone sustained-release hydrogel was tested. (2) A total of 15 Sprague-Dawley rats were used to make liver hemorrhage models. They were randomly divided into five groups: the blank control group used ordinary clean gauze to stop bleeding; the positive control group used Celox hemostatic powder to stop bleeding; the low-, medium- and high-concentration ozone gel groups were covered with ozone sustained-release hydrogel containing ozone concentrations of (3.4±0.2)×10-6, (7.20±0.5)×10-6, and (16.4±1.2)×10-6, and the time of hemostasis was recorded. (3) A total of 15 Sprague-Dawley rats were used to make a model of the back skin wound. They were randomly divided into five groups: the blank control group was not treated; the positive control group was coated with Beijing Wanhong ointment; the low-, medium- and high-concentration ozone gel groups were covered with the ozone sustained-release hydrogel containing ozone concentrations of (2.3±1.8)×10-6, (7.0±3.0)×10-6, and (15.0±5.0)×10-6, and wound healing was observed within 14 days. The experimental project was approved by the animal experimental ethics committee of Wuhan University (approval No. 2019010019). 
RESULTS AND CONCLUSION: (1) Ozone had good stability in hydrogels, which could last for about 2 weeks. (2) In the liver hemostasis experiment, compared with the blank control group, the medium- and high-concentration ozone sustained-release hydrogel and hemostatic powder could shorten the hemostatic time (P < 0.01), and the hemostatic time of the high-concentration ozone sustained-release hydrogel was shorter than that of hemostatic powder (P < 0.05). (3) In the skin wound experiment, compared with the blank control group, medium- and high-concentration ozone sustained-release hydrogel could improve the wound healing rate at 4 days (P < 0.05, P < 0.01); at 14 days, there was no significant difference in wound healing rate among five groups. (4) Ozone sustained-release hydrogel has good stability, can promote hemostasis and wound healing.

Key words: materials, ozone, hydrogel, sustained-release, stability,  hemostasis, wound healing

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