Effect of cryptotanshinone on hypertrophic scar of rabbit ear and its related mechanism

doi:10.3969/j.issn.2095-4344.3221

Abstract

Abstract: BACKGROUND: Cryptotanshinone (CTS) has been shown to have a certain inhibitory effect on hyperplastic scar hyperplasia, but the specific mechanism is still unclear.
OBJECTIVE: To investigate the inhibitory effect of CTS on hypertrophic scar of the rabbit ear and its effect on transforming growth factor-β1/Smads signaling pathway.
METHODS: The rabbit ear model of hypertrophic scar was established and then rabbit models were randomly divided into model group, low and high-dose CTS groups, followed by local injection of normal saline, 27 and 81 mg/L CTS respectively beginning at 21 days after modeling, once a day, for 7 days. Meanwhile, normal rabbits were used as the control group. Scar index was measured after administration. Pathological analysis was carried out by hematoxylin-eosin staining and Masson staining after sampling. The expression of α-smooth muscle actin was detected by immunohistochemistry. Hydroxyproline content was determined by alkaline hydrolysis. The expression of type I collagen, type III collagen and transforming growth factor-β1/Smads signaling pathway related proteins were detected by western blot.
RESULTS AND CONCLUSION: Compared with the control group, the scar index, the number of fibroblasts and collagen fibers were significantly increased, the levels of α-smooth muscle actin and hydroxyproline in scar tissue, and the protein levels of type I collagen, type III collagen, transforming growth factor-β1, p-Smad2, p-Smad3 and Smad4 were significantly increased in the model group (P < 0.05). Compared with the model group, the scar index, the number of fibroblasts and collagen fibers were significantly decreased, the levels of α-smooth muscle actin and hydroxyproline in scar tissue, and the protein levels of type I collagen, type III collagen, transforming growth factor-β1, p-Smad2, p-Smad3 and Smad4 were significantly decreased in the low and high-dose CTS groups (P < 0.05). The above-mentioned parameters changed more significantly in the high-dose CTS group compared to the low-dose CTS group (P < 0.05). To conclude, CTS has a certain inhibitory effect on the formation of hypertrophic scar in rabbit ears, and its mechanism may be related to transforming growth factor-β1/Smads signaling pathway.

Key words: cryptotanshinone, hypertrophic scar, collagen, transforming growth factor-β1, Smads, pathway

CLC Number:

Yang Li, Li Xueli, Song Jinghui, Yu Huiqian, Wang Weixia. Effect of cryptotanshinone on hypertrophic scar of rabbit ear and its related mechanism[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(20): 3150-3155.

Figures/Tables 8

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