Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (29): 4593-4598.doi: 10.3969/j.issn.2095-4344.2810
Shi Fangfu, Cui Hongwang, Sun Bo
Received:
2019-11-18
Revised:
2019-11-21
Accepted:
2020-02-19
Online:
2020-10-18
Published:
2020-09-11
Contact:
Cui Hongwang, MD, Associate chief physician, Department of Spinal Surgery, the First Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, China
About author:
Shi Fangfu, Attending physician, Department of Spinal Surgery, the First Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, China
Supported by:
CLC Number:
Shi Fangfu, Cui Hongwang, Sun Bo. Tumor necrosis factor alpha promotes apoptosis in long bone-like cells via ERK1/2 signaling pathway[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(29): 4593-4598.
2.5 Western blot检测增殖、凋亡蛋白和ERK通路相关蛋白的表达 为了分析肿瘤坏死因子α抑制细胞增殖、促进细胞凋亡的作用机制,采用Western blot检测增殖、凋亡蛋白和ERK通路相关蛋白的表达,结果显示,与对照组相比,肿瘤坏死因子α组和ERK1/2抑制剂组细胞增殖相关蛋白PCNA的表达显著降低,细胞凋亡相关蛋白cleaved caspase-3的表达显著升高。当50 μg/L肿瘤坏死因子α和ERK1/2抑制剂组处理MLO-Y4细胞24 h可明显抑制p-ERK1/2的表达,而总蛋白ERK1/2的表达基本保持不变,与对照组比较,差异有显著性意义(P < 0.05),见图4,5。 "
2.5 Western blot检测增殖、凋亡蛋白和ERK通路相关蛋白的表达 为了分析肿瘤坏死因子α抑制细胞增殖、促进细胞凋亡的作用机制,采用Western blot检测增殖、凋亡蛋白和ERK通路相关蛋白的表达,结果显示,与对照组相比,肿瘤坏死因子α组和ERK1/2抑制剂组细胞增殖相关蛋白PCNA的表达显著降低,细胞凋亡相关蛋白cleaved caspase-3的表达显著升高。当50 μg/L肿瘤坏死因子α和ERK1/2抑制剂组处理MLO-Y4细胞24 h可明显抑制p-ERK1/2的表达,而总蛋白ERK1/2的表达基本保持不变,与对照组比较,差异有显著性意义(P < 0.05),见图4,5。 "
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