Photodynamic antimicrobial chemotherapy for repairing a
rabbit model of osteomyelitis

doi:10.3969/j.issn.2095-4344.2480

Abstract

Abstract:

BACKGROUND: Photodynamic antimicrobial chemotherapy holds broad-spectrum antibacterial ability, fast onset of action and non-resistance, which has been extensively applied in the treatment of superficial localized infection.

OBJECTIVE: To explore the efficacy of photodynamic antimicrobial chemotherapy for treating osteomyelitis.

METHODS: Thirty-six New Zealand white rabbits (provided by Laboratory Animal Center of Academy of Military Medical Sciences) were selected, and a left tibia osteomyelitis model was established. At 28 days after modeling, rabbit models were randomly divided into three groups: blank group (tibia debridement only), control group (vancomycin/polymethylmethacrylate bone cement was filled into the intramedullary cavity of the infectious tibia after debridement), and experimental group (intramedullary treatment of photodynamic antimicrobial chemotherapy after debridement). The gross observation, imaging examination and bacterial culture were performed at 4, 8, and 12 weeks after surgery. The study was approved by the Ethical Committee of Affiliated Hospital of Logistics College of CAPF, with the approval No. (2015)-0002.

RESULTS AND CONCLUSION: (1) Appearance of the skin: purulent secretion was observed in the blank group, but disappeared in the control and experimental groups where the skin healed well. (2) X-ray examination: with time going, the osteomyelitis aggravated in the blank group. The bone destruction was reduced gradually in the control and experimental groups, and the bone defects healed gradually. There was no significant difference between control and experimental groups at different time points after surgery. (3) Bacterial culture: with time increasing, the bacterial positive rate showed no significant change in the blank group. The bacterial positive rate in the control and experimental groups was on a decline, which showed no significant difference between control and experimental groups at different time points after surgery (P > 0.05). (4) These results indicate that photodynamic antimicrobial chemotherapy is a new treatment for osteomyelitis to effectively control infection, providing experimental basis in clinical practice.

Key words: photodynamic antimicrobial biotherapy, photodynamic antimicrobial chemotherapy, vancomycin, polymethylmethacrylate, osteomyelitis, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, resistance

CLC Number:

Ma Jinchao, Liu Tiansheng, Liu Aipeng, Wang Hao, Wang Qi, Liang Yongjian, Wang Lin, Di Haiwei. Photodynamic antimicrobial chemotherapy for repairing a rabbit model of osteomyelitis[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(8): 1254-1259.

Figures/Tables 5

References

[1] SCHMITT SK.Osteomyelitis.Infect Dis Clin North Am.2017; 31(2):325-338.
[2] JERZY K, FRANCIS H.Chronic osteomyelitis bacterial flora，antibiotic sensitivity and treatment challenges.Open Orthop J.2018;30(12):153-163.
[3] SHI P, ZUO Y, LI X, et al.Gentamiei-impregnated chitosan/nanohydmxyapatite/ethyl cellulosemicrospheres granulesforchronic osteomyelitis therapy.J Biomed Mater Res A.2010;93:1020-1031．
[4] SHARMA SK, DAI TH, KHARKWAL GK, et al. Drug Discovery of Antimicrobial Photosensitizers Using Animal Models[J].Curr Pharm Des.2011;17(13):1303-1309.
[5] 滕加文,梁学振,李梁,等.骨髓炎兔胫骨动物模型的建立与评价[J].中国现代药物应用,2017,11(16):194-197
[6] NORDEN CW, MYEROWITZ RL, KELETI E.Experimental osteomyelitis due to staphylococcus aureus or pseudomonas aeruginosa:a radiographic pathological correlative analysis.Br J Exp Pathol.1980;4:451-460.
[7] WANG XH, YU SP, SUN D, et al.Current data on extremities chronic osteomyelitis in southwest China:epidemiology, microbiology and therapeutic consequences.Sci Rep.2017; 7(1):16251.
[8] BISLAND SK, CHIEN C, WILSON BC, et al.Pre-clinical in vitro and in vivo studies to examine the potential use of photodynamic therapy in the treatment of osteomyelitis. Photochem Photobiol Sci.2006;5(1):31-38.
[9] PANTELI M, GIANNOUDIS PV.Chronic osteomyelitis:what the surgeon needs to know.EFORT Open Rev. 2017;1(5): 128-135.
[10] 胥少汀,葛宝丰,徐印坎实用骨科学(4版修订版)[M].郑州:河南科学技术出版社,2018:1562-1567.
[11] 甘先民,侯悦媚,黄乙勇,等.不同成分骨水泥植入对胫骨慢性骨髓炎患者的临床疗效评价[J].中华医院感染学杂志, 2017,27(12): 2763-2766.
[12] 周盼,谢小燕,熊力,等.抗菌光敏剂的分类及研究进展[J].激光生物学报,2017,26(3):193-197.
[13] 彭莹莹,季晓春,王广芬,等.光动力抗菌化学疗法对多药耐药菌及菌膜杀菌效果的研究[J].中华医院感染学杂志, 2018,28(12): 1761-1767.
[14] SPERANDIO FF, HUANG YY, HAMBLIN MR.Antimicrobial photodynamic therapy to kill Gram-negative bacteria.Recent Pat Antiinfect Drug Discov.2013;8(2):108-120.
[15] ORUBA Z, CHOMYSZYN-GAJEWSKA M. Application of photodynamic therapy in dentistry–literature review.Przegl Lek.2016;73(11):857-861.
[16] VON TAPPEINER H JODLBAUER A.On the effect of photody-namic (fluorescent) substances on protozoa and enzymes (in German).Deutsch Arch Klin Med. 1904;39: 427-487．
[17] PARK JH, AHN MY, KIM YC, et al.In vitro and in vivo antimicrobial effect of photodynamic therapy using a highly pure chlorin e6against Staphylococcus aureus Xen29.Biol Pharma Bull.2012;35(4):509-514.
[18] RAGÀS X, SÁNCHEZGARCÍA D, RUIZGONZÁLEZ R, et al. Cationic porphycenes as potential photosensitizers for antimicrobial photodynamictherapy.J Med Chem. 2010;53(21): 7796-803.
[19] ZEINA B, GREENMAN J, CORRY D, et al.Photodynamic effects of antimicrobial photodynamic therapy on keratinocytes in vitro.Br J Dermatol.2002;146:568-573.
[20] SCHASTAK S, ZIGANSHYNA S, GITTER B, et al.Efficient photodynaic therapy against gram-positive and gram-negative bacteria using THPTS,a cationic photosensitizer excited by infrared wavelength.PLoS One. 2010;5:el1674.
[21] CASSIDY CM, DONNELLY RF, TUNNEY MM. Effect of sub-lethal challenge with photodynamic antimicrobial chemotherapy(PACT) on the antibiotic susceptibility of clinical bacterial isolates.J Photochem Photobiol B.2010;99:62-66.
[22] SIMONETTI O, CIRIONI O, ORLANDO F, et al.Effectiveness of antimicrobial photodynamic therapy with a single treatment of RLP068/C1 in an experimental model of Staphylococcus aureus wound infection.Br J Dermatol.2011;164(5):987-995.
[23] GAD F, ZAHRA T, FRANCIS KP, et al.Targeted photodynamic therapy of established soft tissue interactions in mice. Photochem Photobiol Sci.2004;3:451-458.
[24] TARDIVO JP, BAPTISTA MS.Treatment of osteomyelitis in the feet of diabetic patients by photodynamic antimicrobial chemotherapy.Photomed Laser Surg.2009;27:145-150.
[25] 王诗蕾.Ag/ZnO纳米复合材料对口腔S.mutans的作用及机理研究[D].武汉:武汉工程大学,2017.
[26] ETCHEVERRY ME, PASQUALE MA, GARAVAGLIA M. Photodynamic therapy of Hela cell cultures by using LED or laser sources.J Photoch Photobio B.2016;160:271-277.