Expression of perilipin, comparative gene identification-58 and triglyceride in mouse skeletal muscle after 4 weeks of endurance training

doi:10.3969/j.issn.2095-4344.0591

Abstract

Abstract:

BACKGROUND: Comparative gene identification-58 and perilipins (2-5) are expressed in skeletal muscle. There are many studies addressing hormone sensitive lipase regulating steatolysis in skeletal muscle, but changes of triglyceride, perilipin family proteins and comparative gene identification-58 in skeletal muscle during exercise are little reported.
OBJECTIVE: To investigate the effects of 4-week endurance training on the expression of perilipin family proteins and comparative gene identification-58 in C57BL/6J mice, and to explore their correlation with triglyceride level.
METHODS: Eighty 6-week-old male healthy SPF C57BL/6J mice were randomly divided into control group and endurance training group according to the time-points pre-exercise, 1, 2, 3 and 4 weeks post-exercise. The first week of endurance exercise was adaptive training, and the formal training lasted for 4 weeks, 6 days weekly, the training time in the morning 8: 00-12: 00, with 16 m/minute, 5% degree slope for 40 minutes of treadmill exercise. Mice in the control group were allowed free access to food. The body mass and blood indexes were tested. The triglyceride level, expression of perilipin family proteins and comparative gene identification-58 in mouse gastrocnemius and soleus were detected.
RESULTS AND CONCLUSION: Endurance training showed no effect on mouse body mass, blood glucose and free fatty acids, and significantly reduced blood total triglyceride, total cholesterol and insulin levels (P < 0.05), and increased the level of high density lipoprotein cholesterol (P < 0.05). The intramuscular triglyceride of the soleus muscle was higher than that of the gastrocnemius muscle. During the period of endurance training, intramuscular triglycerides firstly reduced and then rose (P < 0.05). In the working muscles (soleus and gastrocnemius) perilipin family proteins (except perlipin 2), comparative gene identification-58 mRNA and protein were significantly increased (P < 0.05). In summary, in the early stage of endurance training, changes in perilipin family proteins (except PLIN2), comparative gene identification-58 mRNA and protein expression participate in the regulation of hydrolysis, and their participation is weakened thereafter, indicating intramuscular triglyceride synthesis on fat is stronger than its hydrolysis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Muscle, Skeletal, Triglycerides, Resistance Training, Tissue Engineering

CLC Number:

R446

Liao Shuaixiong, Zhang Guodong, Song Gang, . Expression of perilipin, comparative gene identification-58 and triglyceride in mouse skeletal muscle after 4 weeks of endurance training[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(3): 354-360.

Figures/Tables 2

2.1 实验动物数量分析实验选用小鼠80只，分为2组，实验过程无脱失，全部进入结果分析。 2.2 耐力运动训练小鼠体质量和血液指标从表2可知，体质量随时间的持续而增长，与训练前相比，训练组和对照组小鼠的体质量都显著地增长(P < 0.05)，但训练组与对照组在训练期的同一时间，体质量差异并无显著性意义 (P > 0.05)；在训练4周末，训练组小鼠体质量显著低于对照组(P < 0.05)。耐力训练期间的血糖、游离脂肪酸(FFA)水平出现无规律的变化，差异无显著性意义(P > 0.05)。血总三酰甘油在训练组随耐力训练时间出现显著性的降低 (P < 0.05)，血总三酰甘油和血总胆固醇在训练组耐力训练的第3，4周显著低于对照组(P < 0.05)。高密度脂蛋白胆固醇在训练第2，3，4周，运动组高于训练前的水平，并与对照组相比差异有显著性意义(P < 0.05)，胰岛素训练组出现显著降低的变化趋势，运动4周与训练前相比，差异有显著性意义(P < 0.05)。 2.3 耐力运动训练小鼠腓肠肌和比目鱼肌肌内三酰甘油水平从表3可知，肌内三酰甘油在比目鱼肌比腓肠肌中含量要高10倍左右。无论是腓肠肌还是比目鱼肌肌内三酰甘油，随着耐力训练的持续，小鼠腓肠肌和比目鱼肌两者的肌内三酰甘油出现先降再升的变化趋势(P < 0.05)，在训练第2周达到低值(P < 0.05)，并在训练第4周达到峰值，与运动前和对照组相比差异有显著性意义(P < 0.05)。 2.4 耐力运动训练小鼠比目鱼肌PLIN家族和CGI-58基因表达从表4可知，耐力训练时，比目鱼肌PLIN3，PLIN5和CGI-58的基因表达出现显著增长的趋势；与训练前相比，峰值出现在训练第4周(P < 0.05)，PLIN3和PLIN5基因表达在训练第2，3和4周，出现显著性的上升(P < 0.05)；CGI-58在训练第3，4周，与训练前比，出现显著性的上升(P < 0.05)。训练组与对照组相比，PLIN3和PLIN5基因表达在训练第2，3，4周显著高于对照组；训练组CGI-58基因表达在训练第3，4周显著高于对照组 (P < 0.05)。耐力训练对PLIN2和PLIN4的影响不具有规律性变化(P > 0.05)。 "

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