Osteogenic effects of heterogeneous dentin particles in conjunction with bone marrow concentrate in the maxillary sinus lift

doi:10.3969/j.issn.2095-4344.0947

Abstract

Abstract:

BACKGROUND: Autologous bone and dentin can be used as bone grafting materials for bone regeneration and reconstruction, but it is unclear whether heterogeneous dentin particles can be processed as bone substitutes to facilitate the bone regeneration.

OBJECTIVE: To investigate the feasibility of undemineralized heterogeneous dentin particles to be used as bone grafting materials, and to compare the effects of bone marrow concentrate and platelet-rich fibrin on bone regeneration in combination with dentin particles.

METHODS: The extracted human teeth were harvested and prepared to be dentin particles for bone grafting through cleaning, crushing, boiling, degreasing and disinfection. Twelve healthy male Beagle dogs, 1 year of age, were selected to create the animal models of bilateral maxillary sinus lift and were then randomly divided into three groups. The maxillary sinus surgery was performed on both sides of each dog. In group A, only implantation of heterogeneous dentine particles was given; in group B, implantation of the mixture of rich platelet fibrin and heterogeneous dentine particles was performed; and in group C, the mixture of bone marrow concentrate and heterogeneous dentine particles was implanted. The specimens from each animal were harvested after 3 months of implantation. Gross observation, X-ray examination and histological analysis were used to evaluate bone regeneration effects.

RESULTS AND CONCLUSION: No inflammatory signs were found in the bone grafting areas of each group. X-ray results showed that dentin particles with high density were detected in the bone graft area of group A, with the clear boundary with the adjacent bone tissue. However, lower density and unclear border of the graft area were detected in the groups B and C. Histological observation revealed that there was less new bone formation with a few multinucleated macrophages around the dentin particles in group A, while in groups B and C, dentin particles with worm-eaten shape was visible, and there was more new bone formation with regular trabecular alignment. The new bone formation rates were (13.61±8.57)%, (32.28±4.85)% and (36.85±9.70)% in group A, group B and group C, respectively. Significantly higher new bone formation rate was found in groups B and C than group A (P < 0.05), but there was no significant difference between group B and group C. Experimental findings show that the undemineralized heterogeneous dentin particles possess good biocompatibility and bone conduction capacity. In combination with bone marrow concentrate and platelet-rich fibrin, the new bone formation around the grafted dentin granules can be effectively promoted. Moreover, bone marrow concentrates have a slightly better effect than platelet-rich fibrin.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Dentin, Bone Marrow, Blood Platelets, Fibrin, Maxillary Sinus, Bone Transplantation, Animal Experimentation, Tissue Engineering

CLC Number:

R318

Cui Ting-ting, Qiu Ze-wen, Shao Yang, Zhong Wei-jian. Osteogenic effects of heterogeneous dentin particles in conjunction with bone marrow concentrate in the maxillary sinus lift[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(30): 4806-4811.

Figures/Tables 2

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