Effect of recombinant human growth hormone on the growth of hair follicle and the expression of related growth factors

doi:10.3969/j.issn.2095-4344.0838

Abstract

Abstract:

BACKGROUND: Alopecia has been shown to be caused by the abnormally inactive state of hair follicle stem cells but not their death.
OBJECTIVE: To confirm the effectiveness of recombinant human growth hormone (rhGH) in promotion of hair regeneration by observing rhGH acting on the hair follicle cultured in vitro, and to explore the possible mechanism at protein level.
METHODS: The individual hair follicle was separated and was randomized into two groups, and then cultured in the serum-free medium containing 0.01, 0.1, 1, 10, 20 and 40 IU/L rhGH, respectively. The cells were cultured in the normal medium served as the control growp. Six individual hair follicles were added into each pore, and incubated in an incubator at 37 ℃ and 5% CO2. Its length was measured using ocular micrometer under inverted contrast microscope daily. Additionally, the expression levels of growth factors were tested after rhGH application.
RESULTS AND CONCLUSION: Compared with the control group, the growth speed of hair follicle was increased at different degrees after different concentrations of rhGH was applied. The optimal concentration of rhGH was 10 IU/L, and then decreased. The length of hair follicle in the 1, and 10 IU/L groups was significantly longer than that in the control group (P < 0.05). The growth curve of hair follicle showed a rising tendency in each group, and 0.1 and 40 IU/L rhGH revealed the similar effect. The largest growth gradient appeared before day 6, followed by a slight increase. rhGH downregulated the expression levels of fibroblast growth factor, transforming growth factor, tumor necrosis factor β and interleukin-21, and upregulated the levels of epidermal growth factor, insulin-like growth factor 2, nerve growth factor, hepatocyte growth factor, and stem cell factor. In summary, rhGH exerts a positive effect on the growth of hair follicle possibly by activating a variety of related growth factors.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Human Growth Hormone, Hair Follicle, Cytokines, Alopecia, Tissue Engineering

CLC Number:

R318

Zhang You-lai, Xin Guo-hua, Zeng Yuan-lin, Jiang Lan, Wang Xiao, Zou Li-jin. Effect of recombinant human growth hormone on the growth of hair follicle and the expression of related growth factors[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(20): 3167-3171.

Figures/Tables 2

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