In vitro isolation, culture and identification of brain tumor stem cells from human astrogliomas

doi:10.3969/j.issn.2095-4344.2013.01.009

Abstract

Abstract:

BACKGROUND: According to the theory of tumor stem cells, there are a small amount of stem cell-like cells that exhibit infinite proliferative potential and self-renewal capacity, can differentiate into cells with the phenotype of mature cells and play a key role in tumor production, proliferation and invasion.
OBJECTIVE: To investigate the feasibility of isolation, culture and identification of brain tumor stem cells from human astrogliomas.
METHODS: Brain tumor stem cells were isolated by primary culture from human astrogliomas. These cells were cultured under the culture condition of neural stem cells. The clone spheres were identified with immunocytochemistery for nestin and CD133. At the same time, differentiated cells were identified by immunocytochemistery for neuron specific enolase, glial fibrillary acidic protein and O4, respectively.
RESULTS AND CONCLUSION: After 7-10 days of culture, a great number of neurospheres immunoreactive for nestin and CD133 were observed. After induced differentiation, these cells were immunoreactive for neuron specific enolase, glial fibrillary acidic protein and O4. These findings suggest that there are brain tumor stem cells with the characteristics of neural stem cells in human astrogliomas. CD133 and nestin are key surface markers for brain tumor stem cells, which can be used for isolation of brain tumor stem cells.

Key words: stem cells, tumor stem cells, astroglioma, brain, CD133, nestin, neuron specific enolase, glial fibrillary acidic protein, cell culture, immunocytochemistry, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Song Xiao-bin, Yang Zhi-yong, Deng Xing-li, Wang Xiang-peng, Zhang Sheng-ping, Huang Jin, Zhang Xing-kui. In vitro isolation, culture and identification of brain tumor stem cells from human astrogliomas[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(1): 56-61.

Figures/Tables 4

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