Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (22): 3476-3482.doi: 10.3969/j.issn.2095-4344.2017.22.006
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Porous Se@SiO2 nanocomposites for treatment of steroid-induced osteonecrosis of the femoral head
- 1Department of Orthopedics, Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, China; 2Department of Orthopedics, Shanghai General Hospital, Shanghai 200080, China
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Received:2017-05-25Online:2017-08-08Published:2017-09-01 -
Contact:Yi Cheng-qing, M.D., Chief physician, Associate professor, Department of Orthopedics, Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, China -
About author:Niu Ke-run, Studying for master’s degree, Department of Orthopedics, Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, China -
Supported by:the National Natural Science Foundation of China, No. 81371979; the Medical Cross Research Fund of Shanghai Jiao Tong University, No. YG2015QN16
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Niu Ke-run, Yang Meng-kai, Ma Chun-hui, Yu Yin-xian, Teng Song-song, Wang Qian, Yi Cheng-qing.
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2.1.1 软骨细胞鉴定结果 Ⅱ型胶原荧光染色阳性见图1A，消化所用的软骨组织甲苯胺蓝染色呈阳性见图1B，表明提取的细胞为软骨细胞。"
2.1.2 活性氧抑制实验结果 活性氧表达由DCFH的荧光强度指示(绿色)。H2O2干预后，实验组软骨细胞经过多孔纳米复合材料Se@SiO2的预刺激，活性氧的表达明显低于对照组，见图2。表明多孔纳米复合材料Se@SiO2可以显著降低活性氧的表达，具有良好的抗氧化应激效果。"
2.1.3 TUNEL实验结果 TUNEL染色阳性的绿色荧光亮点为损伤凋亡的细胞。对照组检测到大量绿色荧光亮点，即代表损伤凋亡的软骨细胞；而实验组检测到极少绿色荧光亮点，见图3。统计学分析实验组凋亡率明显低于对照组(P < 0.05)，见图3。结果表明多孔纳米复合材料Se@SiO2能够保护软骨细胞来自H2O2的氧化应激损伤，降低H2O2引起的软骨细胞凋亡，具有良好的抗氧化应激效果。"
2.2 动物体内实验结果 共纳入36只SD大鼠，无意外死亡，实验标本全部进入结果分析，无脱失。 2.2.1 标本一般形态 取材时观察股骨头标本，对照组股骨头均呈瓷白色，颜色均一，表面光滑，激素诱导组股骨头部分呈暗红色，无光泽，可见股骨头软骨下坏死灶，实验组股骨头大部分呈瓷白色，颜色尚均一，有光泽，个别股骨头表面呈暗红色,见图4。"
2.2.2 Micro CT扫描结果 根据Micro CT扫描二维图像，3组差异明显，激素诱导组股骨头出现大片囊性低密度坏死区；对照组骨形态良好，密度均匀，未发生骨坏死；实验组亦可见少量囊相低密度坏死区，较激素诱导组明显好转，见图5。Micro CT三维扫描，骨密度、骨体积、骨表面积/骨体积、骨小梁数、骨小梁厚度，对照组大于激素诱导组，实验组大于激素诱导组(P < 0.05)；骨小梁分离度对照组小于激素诱导组，实验组小于激素诱导组(P < 0.05)。见图6。结果表明多孔纳米复合材料Se@SiO2可以通过抗氧化应激保护和治疗大鼠激素性股骨头坏死。"
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2.2.3 苏木精-伊红染色结果 对照组正常，骨膜光滑，软骨细胞排列整齐，骨骺排列规则，骨小梁结构完整，未发现明显骨坏死，见图7A。激素诱导组可见大片股骨头表面软骨破坏，骨细胞、软骨细胞排列紊乱，骨小梁断裂，空骨陷窝增多，脂肪细胞增多、肥大，部分融合成泡状，见图7B。实验组在多孔Se@SiO2纳米复合材料注射干预后，大部分染色正常，骨小梁结构尚完整，局部检测到部分骨陷窝增多，部分有脂肪细胞肥大，较激素诱导组明显好转，见图7C。此结果从组织病理学层面说明多孔纳米复合材料Se@SiO2可以通过抗氧化应激保护和治疗大鼠激素性股骨头坏死。"
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