Preparation of silk fibroin/chitosan/nano-hydroxyapatite scaffold for sustained release of bone morphogenetic protein-2

doi:10.3969/j.issn.2095-4344.2017.22.008

Abstract

Abstract:

BACKGROUND: Bone morphogenetic protein-2 (BMP-2) is a key to bone formation and repair. However, it has some disadvantages such as easy to lose and degrade and difficult to sustain continuous effect.

OBJECTIVE: To study the preparation and properties of silk fibroin/chitosan/nano-hydroxyapatite (SF/CS/nHA) scaffold loading BMP-2.

METHODS: After silk degumming, dissolution and purification, 2% SF solution was obtained. BMP-2 was dissolved in 2% CS solution, and then fully mixed with equal volume of SF solution and proper amount of nHA. At last, the SF/CS/nHA scaffold loading BMP-2 was prepared using freeze-drying method as experimental group. The SF/CS/nHA scaffold was soaked in the BMP-2 solution as control group. The scaffold porosity was measured by Archimedes method, the surface morphology of the scaffold was observed by scanning electron microscope, the compressive strength was measured by universal testing machine. Scaffolds in the two groups were soaked in PBS, and the release of BMP-2 was measured by ELISA method at different time points.

RESULTS AND CONCLUSION: (1) The scaffolds in the two groups had irregular porous structure, interconnected pores and uneven pore wall. There was no significant difference between the two groups in mean pore diameter, porosity and maximum compressive strength. (2) On the 1^st day, the release rate of BMP-2 was 4.63% in the experimental group, and the release curve increased slowly. After 28 days, the release curve of BMP-2 was transferred to the plateau stage. But in the control group, the release rate of BMP-2 on the 1^stday was 58.84%, and it was a significant initial burst release. The release curve increased rapidly, and was transferred to the platform stage on the 10^th day. The release rate of BMP-2 release was significantly different between the two groups at days 1, 2, 4, 10 (P < 0.05). These results show that the SF/CS/nHA scaffold loading BMP-2 could sustainably and slowly release BMP-2.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Bone Morphogenetic Proteins, Silk Fibroin, Chitosan, Tissue Engineering

CLC Number:

R318

Huang Wen-liang, Ye Peng, Mo Gang, Tian Ren-yuan, Ma Li-kun, Ruan Shi-qiang, Xu Lin, Deng Jiang.

Preparation of silk fibroin/chitosan/nano-hydroxyapatite scaffold for sustained release of bone morphogenetic protein-2

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(22): 3488-3493.

Figures/Tables 4

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