Effect of angelica polysaccharide on bone marrow hematopoietic stem cells and intercellular adhesion molecule 1 on the surface of stromal cells in mice

doi:10.3969/j.issn.2095-4344.2017.21.003

Abstract

Abstract:

BACKGROUND: Angelica polysaccharide has the function of enriching blood and promoting blood circulation, but how to improve the hematopoietic microenvironment and promote hematopoiesis has become one of the hot topics.

OBJECTIVE: To investigate the effect of angelica polysaccharide on Sca-1⁺, CD34⁺ hematopoietic stem cells and intercellular adhesion molecule 1 level in bone marrow stromal cells in mice.

METHODS: Mouse models of hemorrhagic anemia were made and then randomly divided them into control group, low-concentration angelica polysaccharide group and high-concentration angelica polysaccharide group. Angelica polysaccharide groups were intraperitoneally injected with the corresponding concentration of angelica polysaccharide (4, 6 mg/kg) for continuous 6 days, while the control group was injected with equal amount of normal saline. General conditions of the model mice were observed. Peripheral blood red cells were counted by automatic blood cell analyzer. Bone marrow hematopoietic stem cells were counted by magnetic bead sorting technique and flow cytometry. Proliferation of bone marrow stromal cells was detected by MTT method. Expression of intercellular adhesion molecule-1 in bone marrow stromal cells was detected by flow cytometry.
RESULTS AND CONCLUSION: Angelica polysaccharide had no obvious effect on the body mass of hemorrhagic anemia mice (P > 0.05). The number of peripheral blood cells, the percentage of bone marrow CD34⁺ and Sca-1⁺ cells, the number of bone marrow stromal cells and the level of intercellular adhesion molecule 1 were ranked as follows: high-concentration group > low-concentration group > control group (P < 0.05). To conclude, angelica polysaccharide could promote the proliferation of bone marrow stromal cells and increase the expression of intercellular adhesion molecule 1, thereby promoting the proliferation of hematopoietic stem cells and regulating their functional activities.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Tissue Engineering, Bone Marrow

CLC Number:

R394.2

Wang Gai-qin, Jing Peng, Jia Shu-hua. Effect of angelica polysaccharide on bone marrow hematopoietic stem cells and intercellular adhesion molecule 1 on the surface of stromal cells in mice[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(21): 3293-3298.

Figures/Tables 2

2.1 实验动物数量分析纳入小鼠195只，均进入结果分析，每组每个时间点检测均采用5只，均无死亡和感染，无脱失值。 2.2 当归多糖对小鼠体质量的影响对照组、当归多糖低、高剂量组小鼠不同时间点体质量改变差异无显著性意义(P > 0.05；图1)。 2.3 当归多糖对小鼠外周血中红细胞数的影响对照组、当归多糖低、高剂量组小鼠眼眶静脉采血后0，1 d相比红细胞数均明显降低(P < 0.05)；当归多糖低剂量组采血后5，7，9 d红细胞数高于对照组(P < 0.05)；当归多糖高剂量组采血后3，5，7，9 d红细胞数均高于对照组(P < 0.05)，并且采血后9 d红细胞数恢复正常水平(图2)。 2.4 当归多糖对小鼠骨髓CD34+细胞百分数的影响采用磁珠分选和流式细胞术两种方法分别检测小鼠眼眶静脉采血后不同时间点骨髓CD34+细胞数，结果均显示：当归多糖低剂量组采血后7，9 d小鼠骨髓CD34+细胞百分数较对照组增高(P < 0.05)；当归多糖高剂量组采血后3，7，9 d均较对照组增高(P < 0.05；图3-5)。 2.5 当归多糖对小鼠骨髓Sca-1+细胞百分数的影响小鼠眼眶静脉采血后检测不同时间点骨髓Sca-1+细胞百分数，当归多糖低剂量组采血后7，9 d Sca-1+细胞百分数较对照组增高(P < 0.05)；当归多糖高剂量组采血后3，7，9 d Sca-1+细胞百分数均较对照组增高(P < 0.05，图6-7)。 2.6 当归多糖对小鼠骨髓基质细胞增殖能力的影响骨髓基质细胞体外培养至第3代，流式细胞术检测CD90阳性细胞数为(94.86±4.36)%；CD44阳性细胞数为(95.23± 5.43)%；CD45阳性细胞数为(0.72±0.17)%(图8)。将鉴定后的细胞继续培养，在不同时间点观察骨髓基质细胞的生长情况(图9)。 MTT结果显示：对照组、当归多糖低、高剂量组采血后1 d各组增殖能力差异无显著性意义(P > 0.05)；当归多糖低剂量组采血后5，7，9 d增殖能力较对照组增强(P < 0.05)；当归多糖高剂量组采血后3，5，7，9 d增殖能力均较对照组增强(P < 0.05，图10)。 2.7 当归多糖对小鼠骨髓细胞间黏附分子1表达的影响小鼠眼眶静脉采血后3 d各组小鼠骨髓细胞间黏附分子1表达无明显差异(P > 0.05)；当归多糖低剂量组采血后7，9 d与对照组相比细胞间黏附分子1表达明显增加(P < 0.05)；当归多糖高剂量组采血后7，9 d与对照组相比细胞间黏附分子1升高更明显(P < 0.01，图11-12)。"

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