Collagen type II-modified acellular dermal membrane promotes the repair of cartilage defects

doi:10.3969/j.issn.2095-4344.2017.06.009

Abstract

Abstract:

BACKGROUND: Previous study has observed that the calf acellular dermal membrane exhibits slow repair efficiency, fast degradability speed and other shortcomings in the repair of cartilage defects.
OBJECTIVE: To investigate the repair effect of the collagen type II-modified acellular dermal membrane on cartilage defects in rabbits.
METHODS: The fetal rabbit chondrocytes were seeded onto the collagen type II-modified acellular dermal membrane, and the composite was then observed under scanning electron microscope at 3, 7 and 14 days. Cartilage defect models were established on the bilateral femoral condyles of 24 New Zealand white rabbits, and these model rabbits were randomly allocated to three groups. The cartilage-acellular dermal membrane and cartilage-collagen type II-modified acellular dermal membrane were implanted into the defect regions of control and experimental groups, respectively. Those received no intervention were as blank control group. Collagen type II immunohistochemical staining and Wakitani scoring system were performed at 6 and 12 weeks postoperatively.
RESULTS AND CONCLUSION: Chondrocytes grew and adhered well in the scaffold. The Wakitani scores in the experimental group were significantly lower than those in the control and blank control groups at postoperative 6 and 12 weeks (P < 0.05). At 6 and 12 weeks postoperatively, collagen type II immunohistochemical staining was the strongest in the experimental group, with yellow and brown particles in the cytoplasm; the control group was positive for collagen type II immunohistochemical staining, while the blank control group was negative for the staining. Our findings suggest that the collagen type II-modified acellular dermal membrane is beneficial for the repair of cartilage defects.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Collagen Type II, Cartilage, Tissue Engineering

CLC Number:

R318

Zhang Bai-qing, Zhang Zhong-wen, Sun Lei, Jiang Jian, Wu Xia. Collagen type II-modified acellular dermal membrane promotes the repair of cartilage defects[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(6): 871-876.

Figures/Tables 6

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