Basic fibroblast growth factor-chitosan carrier induces neural stem cells to differentiate into neurons followed by co-culture with myocytes

doi:10.3969/j.issn.2095-4344.2017.06.010

Abstract

Abstract:

BACKGROUND: Neural stem cells (NSCs) hold self-renewal and multi-directional differentiation potential. NSCs differentiation into neurons in high proportion under induction conditions exhibits broad application prospect.
OBJECTIVE: To explore the effect of basic fibroblast growth factor (bFGF)-chitosan carrier on the NSCs differentiation into neurons in vitro, and whether the differentiated neurons could form synaptic-like connection with myocytes.
METHODS: After purification, the NSCs were co-cultured with chitosan, soluble bFGF or bFGF-chitosan carrier. After 7-day induction, the NSCs differentiation into neurons was observed by immunofluorescence staining of beta tubulin III. The NSCs differentiation into cholinergic neurons was observed through double immunofluorescence staining of ChaT and beta tubulin III. The synaptic-like connection between the neurons and myocytes was observed by triple staining of beta tubulin III and MHC.
RESULTS AND CONCLUSION: The percentage of differentiated neurons in the bFGF-chitosan carrier group was 74%, which was significantly higher than that in the other two groups. Additionally, the synaptic-like connection formed between the differentiated neurons and myocytes. To conclude, the bFGF-chitosan carrier promotes the NSCs differentiation into neurons to form synaptic-like connection with the co-cultured myocytes.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

CLC Number:

R318

Zhai Jing-yan, Duan Hong-mei, Shang Jun-kui, Yang Zhao-yang, Li Xiao-guang. Basic fibroblast growth factor-chitosan carrier induces neural stem cells to differentiate into neurons followed by co-culture with myocytes[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(6): 877-882.

Figures/Tables 3

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