Pilose antler polypeptide composite membrane supports a suitable microenvironment for peripheral nerve regeneration

doi:10.3969/j.issn.2095-4344.2013.25.013

Abstract

Abstract:

BACKGROUND: Studies have confirmed that polylactic acid-polyglycolic acid membrane has good biocompatibility, stable mechanical strength, non-toxic side effects and controllable degradation rate. Pilose antler polypeptide contains a variety of active materials, basically promoting DNA synthesis and cell differentiation.
OBJECTIVE: To investigate the repair effect of pilose antler polypeptide-polylactic acid-polyglycolic acid copolymer composite membrane on sciatic nerve injuries in rats.
METHODS: Thirty-six male Wister rats were subject to expose the sciatic nerve after surgery and then randomly divided into sham operation group, control group and experimental group. Rats in the sham operation group received no treatment after removal of the sciatic nerve; those in the control group underwent direct end-to-end nerve anastomosis; while those in the experimental group were wrapped by pilose antler polypeptide-polylactic acid-polyglycolic acid copolymer composite membrane after the end-to-end nerve anastomosis. Four rats were collected from each group, respectively, at postoperative weeks 2, 4, 6 for histological examination, immunohistochemical examination and reverse transcription-PCR detection.
RESULTS AND CONCLUSION: (1) Histological detection: 2, 4, 6 weeks after operation, the regeneration ratios and degree of maturity of neurites ranked as follows: the control group < the experimental group < the sham operation. (2) Immunohistochemical detection: 2, 4, 6 weeks after operation, the transforming growth factor β, insulin-like growth factors antigen dyeing and antigen expression of neurites and myelin sheath ranked as follows: the sham operation group < the control group < the experimental group. (3) Reverse transcription-PCR: 6 weeks after operation, the mRNA expression of transforming growth factor β and insulin-like growth factors was detected as follows: the sham operation group < the control group < the experimental group. These results suggest that pilose antler polypeptide-polylactic acid-polyglycolic acid copolymer composite membrane can provide the necessary microenvironment of nerve regeneration and nerve growth factor, and promote the peripheral nerve regeneration.

Key words: biomaterials, tissue-engineered composite scaffold, pilose antler polypeptide, polylactic acid, polyglycolic acid, composite membrane, peripheral nerve, neural regeneration, National Natural Science Foundation of China

CLC Number:

R318

Wang Ke-li, Lu Lai-jin, Zhang Jing-ling, Li Xiang-jun, Jing Xia-bin . Pilose antler polypeptide composite membrane supports a suitable microenvironment for peripheral nerve regeneration [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(25): 4652-4659.

Figures/Tables 5

References

[1] Lee Sk, Wolfe SW. Peripheral nerve injury and repair. J Am Acad Orthop surg. 2000;4:243-252.

[2] Gu LQ, Pei GX. Beijing: Renmin Junyi Chubanshe. 2001: 70-100.顾立强,裴国献.周围神经损伤基础与临床[M].北京:人民军医出版社,2001:70-100.

[3] Seckel BR. Enhancement of peripheral nerve regeneration. Mucscle Nerve. 1990;13(9):785-791.

[4] Mendell LM. Neurotrophins and sensary neurons: role in development, maintance and injury: a thematic Summary. Philos Trans R Socland B Biol Sci. 1996;351(1338):463-367.

[5] Brushart TM, Mesulam MM. Alteration in connections between muscle and anterior horn motoneurons after peripheral nerve repair. Science. 1980;208(4444):603-605.

[6] Zhang ZH, Ruan HZ. Chuangshang Waike Zazhi. 2002;4(6): 377-380.张泽华,阮怀珍.基因治疗在周围神经损伤修复中的应用[J].创伤外科杂志,2002,4(6):377-380.

[7] Zhao LG, Duan KD. Shengwu Yixue Gongcheng yu Linchuang. 2003;7(2):120-123.赵立国,端康德.神经导管修复周围神经损伤的研究进展[J].生物医学工程与临床.2003,7(2):120-123.

[8] Wang TG, Wang SK, Hong GX, et al. Zhonghua Xianwei Wailke Zazhi. 2001;24:198-200.王同光,王栓科,洪光祥,等.细胞外ATP对外周神经再生作用的实验研究[J].中华显微外科杂志,2001,24:198-200.

[9] Zhong SZ. Zhonghua Chuangshang Zazhi. 2000;16(2):69-70.钟世镇.创伤骨科基础研究展望[J].中华创伤杂志,2000,16(2): 69-70.

[10] Sobue G. Neurotrophic factors and their receptor expressions in human neuropathies. Rinsho Shinkeigaku. 1997;37(12): 1107-1108.

[11] Rutkowski GE, Heath CA. Development of a bioartificiaI nerve graft II Nerve regeneration in vitro. Biotechnol Prog. 2002; 18(2):373-379.

[12] Matsumoto K, Ohnishi K, Kiyotani T, et al. Peripheral nerve regeneration across an 80-mm gap bridged by a polyglycolic acid (PGA)-collagen tube filled with laminin-coated collagen fibers: a histological and electrophysiological evaluation of regenerated nerves. Brain Res. 2000;868(2):315-328.

[13] Agnew SP, Dumanian GA. Technical use of synthetic conduits for nerve repair. J Hand Surg Am. 2010;35(5):838-841.

[14] Lundborg G, Dahlin LB, Danielsen N, et al. Tissue specificity in nerve regeneration. Scand J Hast Recoustr surg. 1986; 20(3):279-283.

[15] Hung V, Dellon AL. Reconstruction of a 4-cm human median nerve gap by including an autogenous nerve slice in a bioabsorbable nerve conduit: case report. J Hand Surg Am. 2008;33(3):313-315.

[16] Sun M, Kingham PJ, Reid AJ, et al. In vitro and in vivo testing of novel ultrathin PCL and PCL/PLA blend films as peripheral nerve conduit. J Biomed Mater Res A. 2010;93(4):1470-1481.

[17] Zhang ZJ, Liu Q, Wang SJ. Yixue Zongshu. 2010;16(5): 647-650.张志军,刘强,王世杰.神经导管的基础与临床研究[J].医学综述, 2010,16(5):647-650.

[18] Huang JF, Zheng HY, Li SP, et al. Zhonghua Shou Waike Zazhi. 2009;25(3):154-157.黄继锋,征华勇,李世普,等.RGD多肽接枝聚复合导管桥接神经缺损的实验研究[J].中华手外科杂志,2009,25(3):154-157.

[19] Ding T, Luo ZJ, Zheng Y, et al. Rapid repair and regeneration of damaged rabbit sciatic nerves by tissue-engineered scaffold made from nano-silver and collagen type I. Injury. 2010;41(5):522-527.

[20] Bushnell BD, McWilliams AD, Whitener GB, et al. Early clinical experience with collagen nerve tubes in digital nerve repair. J Hand Surg Am. 2008;33(7):1081-1087.

[21] Yuan Q, Shah J, Hein S, et al. Controlled and extended drug release behavior of chitosan-based nanoparticle carrier. Acta Biomater. 2010;6(3):1140-1148.

[22] Johansson F, Wallman L, Danielsen N, et al. Porous silicon as a potential electrode material in a nerve repair setting: Tissue reactions. Acta Biomater. 2009;5(6):2230-2237.

[23] Oh SH, Kim JH, Song KS, et al. Peripheral nerve regeneration within an asymmetrically porous PLGA/Pluronic F127 nerve guide conduit. Biomaterials. 2008;29(11): 1601-1609.

[24] Pabari A, Yang SY, Seifalian AM, et al. Modern surgical management of peripheral nerve gap. J Plast Reconstr Aesthet Surg. 2010;63(12):1941-1948.

[25] Jiang X, Lim SH, Mao HQ, et al. Current applications and future perspectives of artificial nerve conduits. Exp Neurol. 2010;223(1):86-101.

[26] Ji Y, Xu GP, Yan JL, et al. Transplanted bone morphogenetic protein/poly(lactic-co-glycolic acid) delayed-release microcysts combined with rat micromorselized bone and collagen for bone tissue engineering. J Int Med Res. 2009; 37(4):1075-1087.

[27] Ling Y, Huang YS, Liang CY. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2008;12(10):1899-1902.凌友,黄岳山,梁常艳.乳酸-羟基乙酸共聚物纳米粒的研究进展[J].中国组织工程研究与临床康复,2008,12(10):1899-1902.

[28] Lee ES, Park KH, Park IS, et al. Glycol chitosan as a stabilizer for protein encapsulated into poly(lactide-co-glycolide) microparticle. Int J Pharm. 2007;338(1-2):310-316.

[29] Yi X, Jin GH, Tian ML, et al. Shenjing Jiepouxue Zazhi. 2007; 23(5):506-510. 衣昕,金国华,田美玲,等.壳聚糖支架与神经神干细胞生物相容性的研究[J].神经解剖学杂志,2007,23(5):506-510.

[30] Rochkind S, Astachov L, el-Ani D, et al. Further development of reconstructive and cell tissue-engineering technology fortreatment of complete peripheral nerve injury in rats. Neurol Res. 2004;26(2):161-166.

[31] Wang KL, Lu LJ, Zhang JL. Zhongguo Zuzhi Gongcheng Yanjiu yu Lingchuang Kangfu Zazhi. 2012;34(16):6307-6311.王克利,路来金,张静玲.聚乳酸和聚羟基乙酸共聚物复合膜修复坐骨神经损伤[J].中国组织工程研究与临床康复杂志,2012, 34(16):6307-6311.

[32] Wei YY, Wang JG, Huang L, et al. Zhonghua Chuangshang Guke Zazhi. 2009;11(1):51-55.魏延云,王建广,黄磊,等.复合神经生长因子的纳米纤维导管促神经再生的初步研究[J].中华创伤骨科杂志,2009,11(1):51-55.

[33] Chen D, Meng XT, Liu JM, et al. Jiepou Xuebao. 2004;35: 240-243.陈东,孟晓婷,刘佳梅,等.鹿茸多肽对胎大鼠脑神经干细胞体外诱导分化的实验研究[J].解剖学报,2004,35:240-243.

[34] Li LJ, Lu LJ, Chen L, et al. Liaoning Zhongyi Zazhi. 2004; 31(4): 343-344.李立军,路来金,陈雷,等.鹿茸多肽促进大鼠坐骨神经再生的实验研究[J].辽宁中医杂志,2004,31(4):343-344.

[35] Sadighi M, Haines SR, Skottner A, et al. Effects of insulin-like growth factor-I (IGF-I) and IGF-II on the growth of antler cells in vitro. J Endocrinol. 1994;143(3):461-469.

[36] Luetteke NC, Lee DC. Transforming growth factor alpha: expression, regulation and biological action of its integral membrane precursor. Semin Cancer Biol. 1990;1(4):265-275.

[37] Liu LL, Cong B, Jiang HM, et al. Techan Yanjiu. 2009;31(2): 60-70.刘琳玲,丛波,姜洪梅,等.鹿茸多肽功能的研究进展[J].特产研究, 2009,31(2):60-70.

[38] Pan FG, Sun W, Zhou Y. Zhongguo Shengwu Zhipin Xue Zazhi. 2007;20(9):669-671.潘风光,孙威,周玉,等.梅花鹿鹿茸多肽的提取及免疫功效的初步研究[J].中国生物制品学杂志,2007,20(9):669-671.

[39] Gu YD. Zhonghua Shou Waike Zazhi. 2000;16(1):7-9.顾玉东. 21世纪臂丛损伤诊治的研究方向与任务[J].中华手外科杂志,2000,16(1):7-9.