Galactosylated alginate and sodium alginate: What is the optimal mass ratio for maintaining the mechanical stability?

doi:10.3969/j.issn.2095-4344.2013.25.012

Abstract

Abstract:

BACKGROUND: Numerous studies showed that material surface modified with galactose can improve adhesion of hepatocytes, and then affect morphology and functional expression of hepatocytes. Microcapsule prepared by electrostatic complex has been widely used in cell encapsulation, enzyme immobilization, drug controlled release and etc. However, there is no report on hepatocytes encapsulated into the microcapsule prepared with chitosan oligomer and alginate sodium.
OBJECTIVE: To prepare a novel galactosylated alginate-chitosan oligomer microcapsule for hepatocytes encapsulation.
METHODS: The synthesized galactosylated alginate was characterized with Fourier transform infrared spectra, nuclear magnetic resonance spectroscopy, and elemental analysis. The solubility of chitosan oligomer was studied by turbidimetric method. The effect of preparation conditions on the microcapsule was finally studied, including content of galactosylated alginate (100%, 50%, 30%, 0) and membrane formation time (5, 10, 20 minutes).
RESULTS AND CONCLUSION: For the synthesis of galactosylated alginate, Fourier transform infrared spectra indicated the disappearance of carboxylic groups and the formation of C-N bonds. Nuclear magnetic resonance spectroscopy suggested that not only the peak corresponding to lactobionic acid appeared in the product, but also some ethyl (dimethylaminopropyl) carbodiimide chains were coupled. According to elemental analysis, the calculated galactose grafting content was 20%, and the coupling ethyl (dimethylaminopropyl) carbodiimide C was 8%. Due to decrease of the negative charge of the alginate molecular chains after coupling, the electrostatic complex membrane formation was affected. The microcapsules possessing mechanical stability can be obtained when the content of galactosylated alginate sodium is lower than 50% in the mixture of galactosylated alginate sodium and alginate sodium.

Key words: biomaterials, biomaterials and controlled drug release, galactose, chitosan oligomer, sodium alginate, microcapsules, preparation, National Natural Science Foundation of China

CLC Number:

R318

Tian Meng, Han Bo, You Chao, Wan Chang-xiu. Galactosylated alginate and sodium alginate: What is the optimal mass ratio for maintaining the mechanical stability? [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(25): 4644-4651.

Figures/Tables 6

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