Inhalation of upconversion nanoparticles does transient damage to the mouse liver and lung

doi:10.3969/j.issn.2095-4344.2016.52.016

Abstract

Abstract:

BACKGROUND: Lots of in vitro experiments have explored the toxicity of upconversion nanoparticles, but its toxicity in vivo is little reported.
OBJECTIVE: To investigate the toxicity of upconversion nanoparticles in mouse organs.
METHODS: After tracheotomy, 36 Balb/c mice were randomly divided into three groups, followed by instilled with 28 mg/kg upconversion nanoparticle (experimental group), the same volume of normal saline (control group), and nothing (sham operation group), respectively. The functional changes of the lung, liver and kidney were detected at 1 day, 1 and 2 weeks postoperatively, and meanwhile, the morphological changes of the lung, liver, kidney, and heart were observed.
RESULTS AND CONCLUSION: At 1 day postoperatively, the pH values in the experimental group were lower than those in the control and sham operation groups (P < 0.05), while the glutamic-pyruvic transaminase level was higher than that in the control and sham operation groups (P < 0.05). The oxygen partial pressure in the sham operation group was higher than that in the other two groups at 1 day postoperatively (P < 0.05). The oxygen partial pressure and glutamic-pyruvic transaminase level did not significantly differ among groups at 1 and 2 weeks postoperatively. The carbon dioxide differential pressure and kidney function showed no significant differences among groups at different time points after surgery. At postoperative 1 day, in the experimental group, hyperplasia and inflammation were most obvious, distorted alveolar cavity and congestion of blood vessels were visible. In the control group, obvious hyperplasia and inflammation were found, the alveolar cavity was crimped and the gap between alveoli was broadened. The sham operation group had normal alveoli with no inflammations. Lung lesions in the experimental and control groups became mild with time at postoperative 1 and 2 weeks. One day postoperatively, hepatocyte swelling and vacuolar degeneration were severer in the experimental group. Moderate hepatocyte swelling and vacuolar degeneration occurred in the control group. The sham operation group showed mild hepatocyte swelling and vacuolar degeneration. The morphology of the liver in each group returned to normal at 2 weeks postoperatively. Fortunately, the heart and kidney structure showed no overt changes in each group. These findings suggest that upconversion nanoparticles cause transient damage to the mouse lung and liver.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Nanoparticles, Mice, Histology, Tissue Engineering

CLC Number:

R318

Yang Run-xiu, Cai Li-yun, Zheng Ai-hua, Guan Ji-tao.

Inhalation of upconversion nanoparticles does transient damage to the mouse liver and lung

[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(52): 7865-7871.

Figures/Tables 6

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