Enzyme digestion method versus tissue block method in the culture of cardiac stem cells

doi:10.3969/j.issn.2095-4344.2016.50.017

Abstract

Abstract:

BACKGROUND: At present, various methods of extracting cardiac stem cells have been reported in China, but the processes of isolation, culture and purification are not yet standardized.
OBJECTIVE: To explore the difference in the culture of rat cardiac stem cells using enzyme digestion method and tissue block method.
METHODS: Cardiac stem cells from Sprague-Dawley rats, clean grade, 1 day of age, were extracted and cultured in vitro using the enzyme digestion method (control group) or tissue block method (experimental group).
RESULTS AND CONCLUSION: Both of the two methods could produce cardiac stem cells, but the proportion of cultured cells in the experimental group was significantly higher than that in the control group, indicating the tissue block method is superior to the enzyme digestion method in the culture of cardiac stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Cell Culture Techniques, Myocardium, Tissue Engineering

CLC Number:

R394.2

Li Sha, Li Shu-ren, Hao Qing-qing, Yang Ling-ling, Zhang Qian-hui, Ma Yu-long, Zhang Yue-hua, Deng Wen-hao. Enzyme digestion method versus tissue block method in the culture of cardiac stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(50): 7565-7570.

Figures/Tables 2

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