Effects of nano porous beta-tricalcium phosphate/collagen scaffold modified with human bone morphogenetic protein 2 gene on differentiation of MC3T3-E1 cell lines

doi:10.3969/j.issn.2095-4344.2016.38.005

Abstract

Abstract:

BACKGROUND: Bone tissue transplantation or osteogenic material filling is after used for bone defect repair. To remove autologous bone tissues can lead to additional damage and secondary deformity, therefore, it is extremely urgent to search for a new osteogenic material.
OBJECTIVE: To construct the porous β-tricalcium phosphate (β-TCP)/collagen scaffold modified with human bone morphogenetic protein 2 (hBMP2) gene, and to observe its effects on differentiation of MC3T3-E1 cell lines.
METHODS: The porous β-TCP/collagen scaffold modified with hBMP2 gene was prepared. Then in vitro culture system of MC3T3-E1 cell lines with composite scaffold was established. There were scaffold and plate groups, and each group was divided into two subgroups according to the different concentrations of plasmid. Samples were collected and observed morphologically by scanning electron microscope and light microscope after complex culture. After 1, 3, 7 and 14 days of induction, calcium nodules were observed through alizarin red staining, the cell cycle was detected by real-time PCR, and expressions of α I-chain collagen type I gene, Osterix and bone sialoprotein were observed.
RESULTS AND CONCLUSION: The number of cells adhered, differentated and distributed on the composite scaffold was significantly higher than that of the single scaffold (P < 0.05). Alizarin red staining and real-time PCR detection showed that the osteogenesis ability of MC3T3-E1 cell lines in the scaffold group was stronger than that in the plate group. To conclude, the porous β-TCP/collagen scaffold modified with hBMP2 gene is an appropriate candidate for bone defect repair.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Bone Morphogenetic Proteins, Collagen, Cell Differentiation, Tissue Engineering

CLC Number:

R318

Ruan Qiang, Zhao Gang, Guo Rui, Xiao Yue, Li Chao.

Effects of nano porous beta-tricalcium phosphate/collagen scaffold modified with human bone morphogenetic protein 2 gene on differentiation of MC3T3-E1 cell lines

[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(38): 5657-5663.

Figures/Tables 5

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