Effects of exercise intervention on serum-related factors and immunity in a rat model of insulin resistance

doi:10.3969/j.issn.2095-4344.2016.27.018

Abstract

Abstract:

BACKGROUND: Type 2 diabetic patients suffered from different levels of insulin resistance and insulin secretion defects. Retinol binding protein 4 is a fat derived factor found in the blood and is considered to be an important target for the treatment of visceral obesity and insulin resistance.
OBJECTIVE: To observe the level of serum inflammatory cytokines and the changes of immune function in insulin resistance model rats after swimming.
METHODS: A total of 28 8-week-old Sprague-Dawley male rat models were randomly divided into swimming group and rest group. Rat received intraperitoneal injection with 3 μg/g recombinant serum retinol binding protein 4, twice a day, with an interval of 12 hours, for 6 weeks. At 6 weeks after administration, rats in the swimming group were subjected to swimming without loading for 6 weeks, 60 minutes per day. Serum retinol binding protein 4 levels were measured with enzyme linked immunosorbent assay (ELISA) kit. Insulin sensitivity (insulin resistance index) was assessed with homeostasis model assessment. Terminal deoxynucleotidyl transferase dUTP nick end labeling method was used to mark the apoptotic cells in rat thymus. ELISA was used to detect serum interleukin-6 expression. ABC-ELISA was used to test Level of serum granulocyte macrophage colony stimulating factor. S-P One Step Method was used to test CD4 and CD8 activities in blood.
RESULTS AND CONCLUSION: Interleukin-6 and granulocyte macrophage colony stimulating factor were significantly positively associated with insulin resistance index. The increase in insulin resistance index would induce the increase in the levels of serum inflammatory cytokine interleukin 6 and granulocyte macrophage colony stimulating factor, but exercises could decrease above levels. Thymus index, CD4, and CD4/CD8 were negatively associated with insulin resistance index. These results indicate that exercise intervention effectively improved immunity function of rats with insulin resistance and reduced insulin resistance index.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Models, Animal, Insulin, Swimming, Retinol-Binding Proteins, Immunity, Tissue Engineering

CLC Number:

R318

Li Qing-he, Wang Jun-min. Effects of exercise intervention on serum-related factors and immunity in a rat model of insulin resistance[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(27): 4075-4082.

Figures/Tables 2

2.1 实验动物数量分析实验纳入28只大鼠，分为3组，全部进入结果分析，无脱落。 2.2 模型稳定性注射视黄醇结合蛋白4制备胰岛素抵抗大鼠模型与人类胰岛素抵抗早期临床症状、病理和化验指标非常相似，适用于药物药效学评价和临床基础研究。 2.3 大鼠血清视黄醇结合蛋白4和胰岛素抵抗指数变化模型安静组、运动干预组血清视黄醇结合蛋白4和胰岛素抵抗指数与空白对照组相比都有了较大变化，但模型安静组上升幅度更为明显，见表1。 2.4 大鼠体质量、胸腺指数、脾脏指数以及CD4、CD8活性变化实验中运动干预组大鼠体质量比模型安静组更大，可能游泳训练有关，在平时喂养过程中运动干预组食量与模型安静组比差异无显著性意义，空白对照组体质量高于模型安静组、运动干预组，猜测可能与两实验组产生了胰岛素抵抗有关。运动干预组、模型安静组的胸腺指数、脾脏指数都呈现相同的变化，运动干预组高于模型安静组，但低于空白对照组；CD4/CD8是反应机体免疫能力的指标，对比发现，模型安静组、运动干预组都有所下降，但运动干预组更接近空白对照组，见表2。 2.5 大鼠胸腺细胞凋亡数量及分布情况视野中空白对照组几乎没有染成棕红色的凋亡细胞，阳性率极低；模型安静组则较空白对照组明显增多；运动干预组则介于二者之间，见图1。 2.6 大鼠血清炎性细胞因子水平变化表3显示，模型安静组白细胞介素6水平最高，而且组内变化幅度很大(标准差数值高)，说明视黄醇结合蛋白4引起的胰岛素抵抗导致机体白细胞介素6的显著增加，相对而言，运动干预组白细胞介素6水平变化相对平稳，稍有升高，但也达到了显著水平。粒细胞-巨噬细胞集落刺激因子与白细胞介素6的变化相似，但绝对数量上没有白细胞介素6变化幅度大。 2.7 大鼠血清白细胞介素6、粒细胞-巨噬细胞集落刺激因子、免疫指标CD4、CD8及视黄醇结合蛋白4之间的关系表4显示，视黄醇结合蛋白4与胰岛素抵抗指数呈显著正相关，与CD4呈显著负相关，尤其是与CD4/CD8呈显著正相关。白细胞介素6、粒细胞-巨噬细胞集落刺激因子均与胰岛素抵抗指数呈显著正相关，但与CD4/CD8无相关性。 2.8 多元线性逐步回归分析以胰岛素抵抗指数水平为因变量，CD4、CD8、CD4/CD8、白细胞介素6、视黄醇结合蛋白4、粒细胞-巨噬细胞集落刺激因子、体质量、胸腺指数、脾脏指数为自变量对运动干预组进行多元线性逐步回归分析，结果表明，胸腺指数、CD4、CD4/CD8是胰岛素抵抗指数的影响因素，回归方程：y=13.501- 11.957×胸腺指数-11.38×CD4/CD8-0.289×CD4。"

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