Adipose-derived mesenchymal stem cells combined with platelet gel for repair of intervertebral disc degeneration in rats

doi:10.3969/j.issn.2095-4344.2013.21.001

Abstract

Abstract:

BACKGROUND: Platelet-rich plasma gel serves as a three-dimensional scaffold in which stem cells can exhibit a three-dimensional growth. Meanwhile, platelet-rich plasma gel can release large amounts of growth factors to promote the proliferation and differentiation of adipose-derived mesenchymal stem cells.
OBJECTIVE: To explore the repair effect of injection of autologous platelet rich plasma-adipose derived mesenchymal stem cells complex on rabbit intervertebral disc degeneration.
METHODS: Rabbit arterial blood was extracted to prepare autologous platelet-rich plasma using secondary centrifugal method, and adipose-derived mesenchymal stem cells were isolated from the fat tissue of rabbit scapular area. Then, adipose-derived mesenchymal stem cells-platelet-rich plasma gel complex was prepared. New Zealand white rabbits were randomized into control group, model group, platelet-rich plasma gel group and complex group (adipose-derived mesenchymal stem cells-platelet-rich plasma gel complex). Intervertebral disc degeneration model was made in the latter three groups using puncture method. At 2 weeks after modeling, corresponding materials were injected into the disc in the four groups, respectively.
RESULTS AND CONCLUSION: After intervertebral disc degeneration in rabbits, the intervertebral gap was significantly reduced, the nucleus pulposus signal was obviously decreased, nucleus pulposus matrix appeared to have a high density and to be deeply stained. The symptoms above mentioned were improved significantly after intervention with the platelet-rich plasma gel or the complex of adipose-derived mesenchymal stem cells-platelet-rich plasma gel, while the latter was better in curative effects. Injection of platelet-rich plasma gel and adipose-derived mesenchymal stem cells-platelet-rich plasma gel complex can reduce the influence of disc degeneration, and the effect of adipose-derived mesenchymal stem cells-platelet-rich plasma gel complex is more outstanding.

Key words: biomaterials, tissue-engineered bone materials, disc degeneration, platelet-rich plasma, adipose-derived mesenchymal stem cells, tissue engineering, nucleus pulposus cells, imaging observation

CLC Number:

R318

Meng Fan-xing, Li Fang, Ye Chao-qun, Yin Yan-bin, Gao Yang. Adipose-derived mesenchymal stem cells combined with platelet gel for repair of intervertebral disc degeneration in rats[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.21.001.

Figures/Tables 5

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