Different components of chicken egg-white extracts affect cell cycle and apoptosis

doi:10.3969/j.issn.2095-4344.2015.46.014

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (46): 7451-7455.doi: 10.3969/j.issn.2095-4344.2015.46.014

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Different components of chicken egg-white extracts affect cell cycle and apoptosis

Ruan Guang-ping^{1, 2, 3}, Yao Xiang^{1, 2, 3}, Liu Ju-fen^{1, 2, 3}, Wang Jin-xiang^{1, 2, 3}, Pang Rong-qing^{1, 2, 3}, Pan Xing-hua^{1, 2, 3}

1The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, Yunnan Province, China; 2Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming 650032, Yunnan Province, China; 3Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming 650032, Yunnan Province, China

Received:2015-09-14 Online:2015-11-12 Published:2015-11-12
Contact: Pan Xing-hua, M.D., Chief physician, the Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, Yunnan Province, China; Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming 650032, Yunnan Province, China; Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming 650032, Yunnan Province, China
About author:Ruan Guang-ping, M.D., Associate chief physician, the Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, Yunnan Province, China; Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming 650032, Yunnan Province, China; Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming 650032, Yunnan Province, China
Supported by:
the National Support Program of China, No. 2014BAI01B01; Yunnan Provincial Science and Technology Project, No. 2013CA005

Abstract

Abstract:

BACKGROUND: If an extract can prolong the S phase and reduce the percentage of apoptosis after co-culture with cells, it is proved that the extract is able to promote cell proliferation.
OBJECTIVE: To prove the effects of chicken egg-white extracts with different components on the proliferation, cell cycle and apoptosis of 293T cells.
METHODS: An ultrafiltration unit was used to separate chicken egg-white extracts into different components that were > 10 ku, < 10 ku, > 3 ku and < 3 ku to co-culture with cells for 3 days. Then, cell proliferation, cell cycle and cell apoptosis were detected.
RESULTS AND CONCLUSION: Chicken egg-white extract components of < 10 ku and < 3 ku could promote cell proliferation, increase the percentage of S-phase cells and reduce the percentage of apoptosis. In conclusion, chicken egg-white extract components that are < 10 ku and < 3 ku can promote cell proliferation, as well as increase the percentage of S-phase cells and reduce apoptosis percentage.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Chickens, Cell Cycle, Apoptosis, Cell Proliferation

Ruan Guang-ping, Yao Xiang, Liu Ju-fen, Wang Jin-xiang, Pang Rong-qing . Different components of chicken egg-white extracts affect cell cycle and apoptosis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(46): 7451-7455.

Figures/Tables 4

References

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Different components of chicken egg-white extracts affect cell cycle and apoptosis

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