Fabricating periodontal tissue engineering compound by gene modified gingival fibroblasts and acellular dermal matrix

doi:10.3969/j.issn.2095-4344.2015.43.004

Abstract

Abstract:

BACKGROUND: Previous studies have found that human platelet-derived growth factor-B (PDGF-B)-transfected gingival fibroblasts are capable of rapid proliferation in vitro, which can secrete platelet-derived growth factor BB proteins.

OBJECTIVE: To explore the ability of PDGF-B-modified gingival fibroblasts in the acellular dermal matrix in vivo to form periodontal tissue engineering compound.

METHODS: Gingival fibroblasts from Beagle dogs transfected with or without PDGF-B gene were implanted into the acellular dermal matrix. Cell growth on the acellular dermal matrix was observed. PDGF-B gene-transfected gingival fibroblasts/acellular dermal matrix composite (experimental group), gingival fibrobalsts/acellular dermal matrix composite (control group) and acellular dermal matrix (blank group) were implanted subcutaneously into the nude mice, respectively. At 2, 4, 8 weeks after implantation, skin tissues were taken and observed histologically.

RESULTS AND CONCLUSION: PDGF-B gene-modified gingival fibroblasts and non-transfected gingival fibroblasts both grew and proliferated well in the acellular dermal matrix. At 8 weeks after implantation, in the blank group, the surrounding cells largely entered into the acellular dermal matrix, but produce less new collagen fibers, and the cells only grew on the original collagen scaffold; in the control group, a great amount of collagen fibers formed, the original collagen fibers in the acellular dermal matrix were replaced by newly formed collagens, but the original collagen structure was reserved; in the experimental group, a large scale of permineralization formed, and mineralized nodes were arranged along the original collagen scaffold. These findings indicate that PDGF-B gene modified gingival fibroblasts can acquire osteoplastic abilities in the acellular dermal matrix in vivo.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Platelet-Derived Growth Factor, Fibroblasts, Tissue Engineering

Zhong Quan, Li Yan-fen, Yan Fu-hua. Fabricating periodontal tissue engineering compound by gene modified gingival fibroblasts and acellular dermal matrix[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(43): 6906-6912.

Figures/Tables 7

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