Techniques and improvements of establishing orthotopic liver transplantation rat models

doi:10.3969/j.issn.2095-4344.2015.40.024

Abstract

Abstract:

BACKGROUND: At present, liver transplantation is the only way to cure end-stage liver disease, but the complications after transplantation is still an important factor of affecting the long-term survival of patients who received orthotopic liver transplantation, therefore it is necessary to establish a stable animal transplantation model.
OBJECTIVE: To establish rat models of orthotopic liver transplantation.
METHODS: After inhalation anesthesia with ether, 204 SD rats were perfused with 2-4 ℃ Ringer’s solution through the abdominal aorta. In order to reduce warm ischemia of the liver, the liver was not turned over before perfusion. The suprahepatic inferior vena cava was cut off along the phrenic ring after perfusion. No further trimming was needed when dressing, so as not to damage the vena cava. The donor liver was removed and preserved in 4℃Ringer’s liquid. The receptor liver was cut off and allogeneic orthotopic liver transplantation was performed using modified two-cuff method. After transplantation, rats could automatically turn over and drink water. Surviving more than 3 days is regarded as a successful transplantation.
RESULTS AND CONCLUSION: 102 liver transplantations were performed in 204 rats, with 86 rats surviving more than 3 days. The success rate of transplantation was 84%. The results demonstrate that rat models of orthotropic liver transplantation can be constructed successfully through improving techniques.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Liver Transplantation, Model, Animal

CLC Number:

R318

Bai Jian-hua, Chen Gang, Zhu Xin-feng, Wang Fan, Zhao Ying-peng, Liu Qi-yu, Li Li. Techniques and improvements of establishing orthotopic liver transplantation rat models[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(40): 6526-6530.

Figures/Tables 1

References

[1] 康永振,沈中阳.肝移植移植后胆道并发症的相关病因分析[J].实用器官移植电子杂志,2013,(1)4:243.
[2] Kashfi A, Mehrabi A, Pahlavan PS, et al. A review of various techniques of orthotopic liver transplantation in the rats. Transplant Proc. 2005;37(1):185-188.
[3] 王轩,杨甲梅,严以群,等.大鼠原位肝移植不同术式的探讨[J].中华器官移植杂志,1998,19(2):76-78.
[4] Lee S, Charters AC, Chandler JG, et al. A technique for orthotopic liver transplantation in the rat. Transplantation. 1973;16(6):664-669.
[5] 傅宇阳,何晓顺,陈剑琳,等.肝组织ICA M-1和LFA-1表达对急性排斥反应的诊断价值[J].中国普通外科杂志,2002,11(1):37-40.
[6] Yamaguchi Y, Halperin EC, Harland RC, et al. Significant prolongation of hamster liver transplant survival in Lewis rats by total-lymphoid irradiation, cyclosporine,and splenectomy.Transplantation. 1990;49(1):13-17.
[7] 沈中阳.正视发展中的中国肝脏移植[J].中国危重病急救医学, 2010,22(1):2-3.
[8] Wakiya T, Sanada Y, Mizuta K, et al. Interventional radiology for hepatic artery complications soon after living donor liver transplantation in a neonate. Pediatr Transplant. 2012;16(3): E81-E85.
[9] 李志伟,李超,马继韬,等.血型不相容肝移植新型策略临床的应用[J].中国普通外科杂志,2011,20(1):6-10.
[10] 巫北海,周代全,蔡萍等.活体形态学腹盆上卷[M].北京:科学出版社,2006,4:193.

[11] de Ville de Goyet J, Hausleithner V, Malaise J, et al. Liver procurement without in situ portal perfusion. A safe procedure for more flexible multiple organ harvesting. Transplantation. 1994;57(9):1328-1332.
[12] Cherqui D, Hingot JL, Humeres R, et al. Harvesting of the liver without in situ cannulation of the portal system. J Am Coll Surg. 1994;178(6):622-623.
[13] Schemmer P, Enomoto N, Bradford BU, et al. Activated Kupffer cells cause a hypernetabolic state after gentle in situ manipulation of liver in rats. Am J physiol Gastrointest Liver Physiol. 2001;280(6):G1076-G1082.
[14] Schemmer P, Schoonhoven R, Swenberg JA, et al. Gentle in situ liver manipulation during organ harvest decrease survival after rat liver transplantation. Transplantation. 1998;65(8): 1015-1020.
[15] 王爽,高毅,孙尔维,等.双袖套法大鼠原位肝移植术的改进[J].广东医学,2001,22(9):783-785.
[16] 陈绪贵,王卓强,石炳毅,等.肝脏移植手术期间机体组织氧代谢和乳酸的变化[J].解放军医学杂志,2003,28(8):710-711.
[17] Guo Y, Feng L, Zhou Y, et al. Systematic review with meta-analysis: HIF-1α attenuates liver ischemia-reperfusion injury. Transplant Rev (Orlando). 2015;29(3):127-134.
[18] Zhuonan Z, Sen G, Zhipeng J, et al. Hypoxia preconditioning induced HIF-1α promotes glucose metabolism and protects mitochondria in liver I/R injury. Clin Res Hepatol Gastroenterol. 2015.
[19] Ferrigno A, Di Pasqua LG, Bianchi A, et al. Metabolic shift in liver: correlation between perfusion temperature and hypoxia inducible factor-1α. World J Gastroenterol. 2015;21(4): 1108-1116.
[20] Teoh NC, Ajamieh H, Wong HJ, et al. Microparticles mediate hepatic ischemia-reperfusion injury and are the targets of Diannexin (ASP8597). PLoS One. 2014;9(9):e104376.
[21] Jiang W, Kong L, Ni Q, et al. miR-146a ameliorates liver ischemia/reperfusion injury by suppressing IRAK1 and TRAF6. PLoS One. 2014;9(7):e101530.
[22] Zhang C, Huang C, Tian Y, et al. Polyol pathway exacerbated ischemia/reperfusion-induced injury in steatotic liver. Oxid Med Cell Longev. 2014;2014:963629.
[23] Wang Y, Li C, Cheng K, et al. Activation of liver X receptor improves viability of adipose-derived mesenchymal stem cells to attenuate myocardial ischemia injury through TLR4/NF-κB and Keap-1/Nrf-2 signaling pathways. Antioxid Redox Signal. 2014;21(18):2543-2557.
[24] Akhtar MZ, Sutherland AI, Huang H, et al. The role of hypoxia-inducible factors in organ donation and transplantation: the current perspective and future opportunities. Am J Transplant. 2014;14(7):1481-1487.
[25] Nativ NI, Yarmush G, So A, et al. Elevated sensitivity of macrosteatotic hepatocytes to hypoxia/reoxygenation stress is reversed by a novel defatting protocol. Liver Transpl. 2014; 20(8):1000-1011.
[26] Orci LA, Lacotte S, Oldani G, et al. The role of hepatic ischemia-reperfusion injury and liver parenchymal quality on cancer recurrence. Dig Dis Sci. 2014;59(9):2058-2068.
[27] Huang H, Hou M, Xu J, et al. Roles of heme oxygenase-1 promoting regeneration of peribiliary vascular plexus in bile duct ischemia/reperfusion injury. Zhonghua Wai Ke Za Zhi. 2014;52(3):193-197.
[28] Tao T, Chen F, Bo L, et al. Ginsenoside Rg1 protects mouse liver against ischemia-reperfusion injury through anti-inflammatory and anti-apoptosis properties. J Surg Res. 2014;191(1):231-238.
[29] Oldani G, Crowe LA, Orci LA, et al. Pre-retrieval reperfusion decreases cancer recurrence after rat ischemic liver graft transplantation. J Hepatol. 2014;61(2):278-285.
[30] Yan H, Jihong Y, Feng Z, et al. Sirtuin 1-mediated inhibition of p66shc expression alleviates liver ischemia/reperfusion injury. Crit Care Med. 2014;42(5):e373-e381.
[31] Morrissey PE, Monaco AP. Donation after circulatory death: current practices, ongoing challenges, and potential improvements. Transplantation. 2014;97(3):258-264.
[32] Cheng L, Tian F, Tian F, et al. Repression of Farnesoid X receptor contributes to biliary injuries of liver grafts through disturbing cholangiocyte bile acid transport. Am J Transplant. 2013;13(12):3094-3102.
[33] Zimmerman MA, Kam I, Eltzschig H, et al. Biological implications of extracellular adenosine in hepatic ischemia and reperfusion injury. Am J Transplant. 2013;13(10): 2524-2529.
[34] Ke B, Shen XD, Zhang Y, et al. KEAP1-NRF2 complex in ischemia-induced hepatocellular damage of mouse liver transplants. J Hepatol. 2013;59(6):1200-1207.
[35] Zimmerman MA, Grenz A, Tak E, et al. Signaling through hepatocellular A2B adenosine receptors dampens ischemia and reperfusion injury of the liver. Proc Natl Acad Sci U S A. 2013;110(29):12012-12017.
[36] Zimmerman MA, Tak E, Ehrentraut SF, et al. Equilibrative nucleoside transporter (ENT)-1-dependent elevation of extracellular adenosine protects the liver during ischemia and reperfusion. Hepatology. 2013;58(5):1766-1778.
[37] 周立红,朱跃辉.大量输血后血清电解质及酸碱平衡的变化[J].临床急诊杂志,2008,9(2):80-81.