Construction of rat models of acute high altitude stress-induced gastrointestinal injury

doi:10.3969/j.issn.2095-4344.2015.40.015

Abstract

Abstract:

BACKGROUND: At present, there are few reports about construction of the model of acute high altitude stress-induced gastrointestinal injury. Construction of a stable high altitude stress-induced gastrointestinal injury model has become a premise of the current studies on acute stress-induced gastrointestinal injury and related diseases.

OBJECTIVE: To establish rat models of acute high altitude stress-induced gastrointestinal ischemia/reperfusion injury.

METHODS: Twenty-four male Wistar rats were randomly divided into normal control (lower limit of high altitude) , sham (high altitude) and ischemia/reperfusion (high altitude) groups (n=8 rats/group). Rats from all groups underwent a rush for high altitude region. The root of the superior mesenteric artery of rats in the ischemia/reperfusion group was clamped by a vascular clip to completely block blood flow for 60 minutes, and then intestinal blood flow was recovered for reperfusion for 60 minutes. The rats in the sham group were only
subjected to separation of superior mesenteric artery without occlusion. The rats in the normal control group received no treatment. After reperfusion for 60 minutes, serum levels of creatine kinase isoenzyme, aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen and creatinine were detected by chemiluminescence assay and histopathological analysis was performed.

RESULTS AND CONCLUSION: A rush for high altitude region combined with gastrointestinal ischemia/reperfusion damages rat liver, kidney and heart function to different degrees, leading to intestinal mucosal bleeding, glands rupture or even dissolution, and infiltration of a large number of inflammatory cells in the mucous layer and lamina propria layer. These results suggest that the method of preparing rat models of acute high altitude stress-induced gastrointestinal injury by clamping the root of the superior mesenteric artery is simple to operate. The constructed rat models have a relatively stable disease condition and are suitable for investigating the strategies for preventing and treating acute high altitude stress-induced gastrointestinal injury.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Model, Animal, Reperfusion Injury, Altitude

CLC Number:

R318

Yang Zhi-hua, Wang Kun, Ha Xiao-qin, Zhang Ping. Construction of rat models of acute high altitude stress-induced gastrointestinal injury[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(40): 6475-6479.

Figures/Tables 3

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