The neuroprotective effect of astaxanthin on newborn rat models of hypoxic-ischemic brain damage

doi:10.3969/j.issn.2095-4344.2015.40.016

Abstract

Abstract:

BACKGROUND: Several studies have demonstrated that astaxanthin has a good neuroprotective effect; however, the treatment effects of astaxanthin on newborns with hypoxic-ischemic brain damage have not been reported.

OBJECTIVE: To build newborn rat models of hypoxic-ischemic brain damage, and investigate the neuroprotective effects of astaxanthin and the ways of action.

METHODS: Thirty newborn Sprague-Dawley rats aged 7 days out of 98 were randomly taken as sham-operated group. The rest of rats were subjected to ligature of the left carotid artery for 2 hours and then placed in the hypoxic box containing 92% special standard gas and 8% oxygen to establish the models of hypoxic-ischemic brain damage. The rats in the sham-operated group only underwent separation of the carotid arteries, without hypoxic-ischemic treatment. Rat models of hypoxic-ischemic brain damage were randomly divided into hypoxic-ischemic brain damage and astaxanthin-treated groups, with 30 rats in each group. The rats in the astaxanthin-treated group were intraperitionally injected with 80 mg/kg astaxanthin after hypoxic-ischemic brain damage.

RESULTS AND CONLUSION: Compared with the sham-operated group, the expression levels of p-Akt, p-GSK3β and cleaved-caspase-3 protein in the parietal cortex of ischemic damage area of rats were significantly increased, whereas Bcl-2 protein expression was significantly decreased in the hypoxic-ischemic brain damage group (all P < 0.05). Compared with the hypoxic-ischemic brain damage group, astaxanthin treatment significantly reduced the expression level of apoptosis-related protein cleaved-caspase 3 protein (P < 0.05), significantly increased the expression level of Bcl-2 protein (P < 0.05), and significantly reduced the number of apoptotic cells in the hypoxic-ischemic brain area(P < 0.05). These findings suggest that astaxanthin can distinctly improve the prognosis and ways of action of hypoxic-ischemic brain damage in newborn rats which associates with up-regulation of Akt/GSK3β signaling pathways.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Cerebral Hypoxic-Ischemia, Carotenoids, Newborn Animal, Brain Injures

CLC Number:

R318

Lin Liang-feng. The neuroprotective effect of astaxanthin on newborn rat models of hypoxic-ischemic brain damage [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(40): 6480-6484.

Figures/Tables 2

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