The anti-aging study of human umbilical cord mesenchymal stem cells combined with lycopene treatment in the aging beagles

doi:10.3969/j.issn.2095-4344.2014.45.005

Abstract

Abstract:

BACKGROUND: Previous tudies have shown that the anti-aging effects of stem cells with lycopene are more significant, and can also significantly improve the aging body immune function.

OBJECTIVE: To investigate the anti-aging effects of human umbilical cord mesenchymal stem cells combined with lycopene on the aging beagles.

METHODS: Sixteen aging beagles (6-7 years old) were randomly divided into two groups: aging control group and aging treatment group; young beagles (3-4 years old) were chosen as young control group. In the aging treatment group, human umbilical cord mesenchymal stem cells combined with lycopene was given; while in the other two group, an equal amount of DMEM/F12 cell culture medium and sunflower oil was given. Each dog's general conditions were observed regularly during the whole progress. The changes of superoxide dismutase, malondialdehyde, glutathione peroxidase in the serum were detected at regular time of the whole process, and the structure changes of each organ were observed at 24 weeks of treatment.

RESULTS AND CONCLUSION: (1) Before treatment, the levels of superoxide dismutase and glutathione peroxidase in the aging control and treatment groups were lower than those in the young control group (P < 0.05), while the malondialdehyde content was higher than that in the young control group (P < 0.05). However, there was no significant statistical difference between aging control and treatment groups (P > 0.05). (2) For the aging treatment group at 24 weeks of treatment: the beagle fur became clearer and smoother, motility was strengthened, appetite became better; and the activity of superoxide dismutase in serum at 8 to 24 weeks of treatment increased significantly compared with before treatment (P < 0.05), the activity of glutathione peroxidase significantly increased from 6 weeks (compared with treatment before, P < 0.05), the malondialdehyde content decreased significantly from 4 weeks of treatment to the completion of the experiment (compared with before treatment, P < 0.05). (3) After the experiment, the microscopic observation showed that compared with the aging control group, the tissues and organ structures of the aging treatment group were all clear, had no inflammatory infiltrates, no obvious necrosis and fibrosis lesions. These results were mainly consistent with the observations of young control group. The above results show that umbilical cord mesenchymal stem cells combined with lycopene therapy on the natural aging beagles may enhance the activities of antioxidant enzymes, reduce malondialdehyde content, and their combination also can repair tissue structures and promote the functions, which has obvious anti-aging effects.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: umbilical cord, mesenchymal stem cell transplantation, aging, carotenoids

CLC Number:

R394.2

Wang Lin-lin, Cui Xiao-lan, Shi Han, Cheng Xue, Liu Jia, Shen Yi, Li Qian-qian, Wang Yi-zhong. The anti-aging study of human umbilical cord mesenchymal stem cells combined with lycopene treatment in the aging beagles[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7239-7245.

Figures/Tables 7

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