Effect of Buyang Huanwu Decoction combined with bone marrow mesenchymal stem cell transplantation on expression of tight junction proteins in the brain of cerebral ischemia-reperfusion rats

doi:10.3969/j.issn.2095-4344.1526

Abstract

Abstract:

BACKGROUND: In acute cerebral ischemia, the tight junction between endothelial cells is damaged, and variation in the transcription or expression of tight junction proteins increases the blood-brain barrier permeability eventually triggering the formation of brain edema.
OBJECTIVE: To explore the effect mechanism by which Buyang Huanwu Decoction combined with bone marrow mesenchymal stem cell (BMSCs) transplantation treats a cerebral ischemia-reperfusion rat model via the blood brain barrier.
METHODS: Eighty rats provided by the Animal Experimental Central of Shandong Province, China, were randomized into sham operation group (n=16), model group (n=16), transplantation group (n=16), Buyang Huanwu Decoction group (n=16), and Buyang Huanwu Decoction+transplantation group (combination group, n=16). Ischemia-reperfusion rat model was established via middle cerebral artery occlusion using improved Zea Longa’s suture-occlusion method. In the Buyang Huanwu Decoction group and combination group, the rats were gavaged with 15 ml/kg Buyang Huanwu Decoction 3 days before modeling, while in the sham operation group, model group and transplantation group, the rats were gavaged with the equal volume of normal saline. The transplantation group and combination group were injected with bone marrow mesenchymal stem cell suspension (1x10⁶ mL, 1 mL) 1 day after modeling, and the other groups were gavaged with the equal volume of normal saline. The expression of Occludin, Claudin-5, Jam-A, and ZO-1 in ischemic brain tissues was detected by western blot assay at 3 and 5 days after modeling.
RESULTS AND CONCLUSION: Compared with the sham operation group, the protein expression of Occludin, Claudin-5, Jam-A, and ZO-1 in the ischemic brain tissue of rats in the model group decreased significantly (P < 0.01). Compared with the model group, the protein expression levels of Occludin, Claudin-5, Jam-A, and ZO-1 in the rat ischemic brain tissue in the Buyang Huanwu Decoction group, transplantation group and combination group were up-regulated significantly (P < 0.05), with the most significant increase in the combination group (P < 0.01). Buyang Huanwu Decoction combined with bone marrow mesenchymal stem cell transplantation exerts a synergistic effect in blood-brain barrier repair, and keeps the tight junction with the blood-brain barrier through the upregulation of ischemic brain tissue Occludin, Claudin-5, Jam-A and ZO-1protein expression, to achieve the brain protection.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Infarction, Middle Cerebral Artery, Reperfusion Injury, Bone Marrow, Mesenchymal Stem Cell Transplantation, Claudins, Tissue Engineering

CLC Number:

Zhang Yunke, Che Zhiying, Li Ke. Effect of Buyang Huanwu Decoction combined with bone marrow mesenchymal stem cell transplantation on expression of tight junction proteins in the brain of cerebral ischemia-reperfusion rats[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(1): 55-60.

Figures/Tables 6

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