Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (8): 1229-1234.doi: 10.3969/j.issn.2095-4344.0141

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Hydrogen rich water protects against myocardial ischemia/reperfusion injury in rats

Wang Zan1, Liu Li1, Zhang Zhe1, Sun Xiao-yu1, Li Xiang-zi1, Li Zhi-lin2, Liu Fu-lin3, Zhou Yu-juan1   

  1. 1School of Medicine, 2School of Chemistry, Hebei University, Baoding 071000, Hebei Province, China; 3Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
  • Received:2017-10-07 Online:2018-03-18 Published:2018-03-18
  • Contact: Zhou Yu-juan, Master, Professor, Master’s supervisor, School of Medicine, Hebei University, Baoding 071000, Hebei Province, China Corresponding author: Liu Fu-lin, Master, Chief physician, Professor, Master’s supervisor, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
  • About author:Wang Zan, Master candidate, School of Medicine, Hebei University, Baoding 071000, Hebei Province, China
  • Supported by:

    the Key Medicine Research Program of Hebei Province in 2013, No. 20130369

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Abstract:

BACKGROUND: Increasing evidence has indicated that low-concentration hydrogen or hydrogen rich water or hydrogen saturated saline exerts a protective effect on various diseases, such as myocardial ischemia/reperfusion injury.
OBJECTIVE: To explore the protective effect of hydrogen rich water on myocardial ischemia/reperfusion injury.
METHODS: Forty-eight Wistar rats were equally randomized into control and hydrogen-rich groups, and then subdivided into ischemic preconditioning, ischemia, and ischemia/reperfusion groups (n=8 rats in each subgroup). The myocardial ischemia/reperfusion model was established in the heart of each rat by the following procedures: reverse perfusion for 10 minutes, room temperature for 20 minutes, and reperfusion for 20 minutes. The control rats was perfused with pre-oxygenated (95% O2 plus 5% CO2) 37 ℃ K-R solution and the hydrogen-rich group was perfused with pre-oxygen-equilibrated (95% O2 plus 5% CO2) 37 ℃ K-R solution plus hydrogen-rich water      (0.6 mmol/L, pH=7.3). Subsequently, the heart was removed, the pathological changes of the myocardial tissues were observe by hematoxylin-eosin staining, the activities of lactic dehydrogenase and creatine kinase in the myocardial tissues were determined, and the levels of tumor necrosis factor-α and interleukin-1β were detected by ELISA.
RESULTS AND CONCLUSION: In the control group, the activity of lactic dehydrogenase at the ischemic and ischemia/reperfusion stages was significantly higher than that at the ischemic preconditioning stage (P < 0.05), and the activity of creatine kinase at the ischemia/reperfusion stage was significantly higher than that at the ischemic preconditioning and ischemic stages (P < 0.05). In the hydrogen-rich group, there was no significant difference in the activities of lactic dehydrodenase and creatine kinase at each stage, but the activities of at the ischemia/reperfusion stage was significantly lower than those in the control group (P < 0.05). In the two groups, the order of the levels of tumor necrosis factor-α and interleukin-1β was as follows: the ischemia/reperfusion stage > ischemic stage > ischemic preconditioning stage (P < 0.05). The levels of above factors in the hydrogen-rich group were significantly lower than those in the control group (P < 0.05). Our findings imply that hydrogen rich water has protective effect on myocardial ischemia/reperfusion injury of the rat hearts in vitro, which may be by reducing the expression of tumor necrosis factor-α and interleukin-1β, and further alleviating the inflammatory response.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Hydrogen, Myocardium, Reperfusion Injury, Creatine Kinase, Tissue Engineering

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