Osteogenesis of adipose stem cells on artificial bone scaffold

doi:10.3969/j.issn.2095-4344.2015.28.012

Abstract

Abstract:

BACKGROUND: Insufficient source of seed cells is the important factor to restrict the tissue reconstruction and the development of regenerative medicine.

OBJECTIVE: To explore the osteogenesis of adipose stem cells cultured with different kinds of artificial bones.

METHODS: Adipose tissue was extracted from female volunteers undergoing cosmetic surgery to isolate adipose stem cells. Passage 4 adipose-derived mesenchymal stem cells were selected and divided into osteogenic induction group, osteogenic induction+hydroxyapatite group, osteogenic induction+absorbable bone group and osteogenic induction+recombinant bone xenograft group. The latter three groups were subdivided into 3, 10,

20 g/L subgroups, respectively. Culture medium was exchanged every 3 days, totally for 12 days.

RESULTS AND CONCLUSION: Compared with the osteogenic induction group, the calcium concentration in the elution liquid was significantly higher in the osteogenic induction+hydroxyapatite group and low-concentration osteogenic induction+absorbable bone group (both P < 0.05), but no difference was found between the osteogenic induction group and high-concentration osteogenic induction+absorbable bone group (P < 0.05). In addition, the calcium concentration in the elution liquid was significantly lower in the osteogenic induction+ recombinant bone xenograft group than the osteogenic induction (P < 0.05). Therefore, different artificial bone scaffolds can influence the osteogenic effect of adipose stem cells, and among them, hydroxyapatite has a better effect on the osteogenic induction of adipose stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Tissue Scaffolds, Subcutaneous Fat, Stem Cells, Hydroxyapatites

CLC Number:

R394.2

Su Peng, Zhang Wei-wei, Yu Hua-wei, Yang Han. Osteogenesis of adipose stem cells on artificial bone scaffold [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(28): 4493-4497.

Figures/Tables 2

References

[1] 张健,杨晶,杨光艳,等.脂肪干细胞与人工骨混合后的成骨作用研究[J].组织工程与重建外科杂志,2012,08(3):135-139.

[2] 彭华志.体外诱导脂肪来源干细胞向成骨细胞分化的相关研究[D].西安:第四军医大学,2007.
[3] 赵振德.人工骨联合骨髓间充质干细胞修复兔股骨头坏死[J].中国组织工程研究,2012,16(38):7057-7062.
[4] 张健,杨晶,杨光艳,等.脂肪干细胞与人工骨混合后的成骨作用研究[J].组织工程与重建外科,2012,8(3):135-139.
[5] 刘喜民,田志逢,张小玲,等.骨基质明胶/煅烧骨基质重组人工骨对骨髓间充质干细胞成骨潜能的影响[J].中国组织工程研究, 2012,16(25):4566-4570.
[6] 周永胜,刘云松,葛雯姝,等.人脂肪基质细胞在骨组织工程学中的应用[J].北京大学学报(医学版),2012,44(1):160-162.
[7] 戴文达,林红,方涛林,等.人骨髓间充质干细胞结合多孔β-TCP构建生物相容性人工骨[J].中国生物医学工程学报,2009,28(1): 108-116.
[8] 张钦.自体脂肪源性干细胞复合异种松质骨支架用于修复兔颅骨缺损的实验研究[D].西安:第四军医大学,2008.
[9] 刘兆杰,胡永成.外源性生长因子修饰脂肪组织来源干细胞成骨与成软骨表达的研究进展[J].中国矫形外科杂志,2013,21(3): 250-255.
[10] 韩立强.人体脂肪基质细胞的分离培养及其成骨活性的研究[D].天津:天津医科大学,2006.
[11] Shizaka R, Iohara K, Murakami M, et al. Regeneration of dental pulp following pulpectomy by fractionated stem/progenitor cells from bone marrow and adipose tissue. Biomaterials. 2012;33(7):2109-2118.
[12] Song K, Wang Z, Li W, et al. In vitro culture, determination, and directed differentiation of adult adipose-derived stem cells towards cardiomyocyte-like cells induced by angiotensin II. Appl Biochem Biotechnol. 2013;170(2):459-470.
[13] 黄成龙,肖金刚.脂肪干细胞成骨分化及与复合支架结合:在修复骨质疏松症骨缺损中的应用[J]. 中国组织工程研究,2014, 18(41): 6696-6702.
[14] Ercan E, Bagla AG, Aksoy A, et al. In vitro protection of adipose tissue-derived mesenchymal stem cells by erythropoietin. Acta Histochem. 2014;116(1):117-125.
[15] 程文丹.人脂肪干细胞基本生物学特性、表面抗原及成骨、成脂分化潜能的研究[D].合肥:安徽医科大学,2008.
[16] 韩宁波.结合有TGF-β1的肝素化胶原/壳聚糖支架复合脂肪干细胞修复兔关节软骨缺损的研究[D].上海:第二军医大学, 2009.
[17] 张健,杨晶,杨光艳,等.脂肪干细胞与人工骨混合后的成骨作用研究[J].组织工程与重建外科杂志,2012,8(3):135-139.
[18] 叶前驱,熊德建,陈森荣.经伤椎置钉并植入人工骨复合物治疗胸腰椎骨折[J].实用骨科杂志,2013,19(6):537-539.
[19] 吕鹏飞,张光武.脐带间充质干细胞在骨组织工程中的研究进展[J].中华临床医师杂志(电子版),2013,7(10):140-141.

[20] 赵刚,丁家华,陈宝安,等.异基因造血干细胞移植后股骨头坏死二例[J].江苏医药,2012,38(18):2224-2225.
[21] 王斌,吕浩然,王伟雄,等.异体皮质人工骨复合物治疗骨折不愈合及骨缺损[J].中国组织工程研究,2013,17(51):8821-8826.
[22] Ma J, Both SK, Ji W, et al. Adipose tissue-derived mesenchymal stem cells as monocultures or cocultures with human umbilical vein endothelial cells: performance in vitro and in rat cranial defects. J Biomed Mater Res A. 2014; 102(4):1026-1036.
[23] Khosravizadeh Z, Razavi S, Bahramian H, et al. The beneficial effect of encapsulated human adipose-derived stem cells in alginate hydrogel on neural differentiation. J Biomed Mater Res B Appl Biomater. 2014;102(4):749-755.
[24] Hsueh YY, Chang YJ, Huang TC, et al. Functional recoveries of sciatic nerve regeneration by combining chitosan-coated conduit and neurosphere cells induced from adipose-derived stem cells. Biomaterials. 2014;35(7):2234-2244.
[25] 刘智.脂肪与骨髓来源的成骨细胞成骨活性对比研究[D].天津:天津医科大学,2007.
[26] 林春阳.组织工程人工骨成骨活性因子(BMP9,OPG)相关研究[D].重庆:重庆医科大学,2011.
[27] 郝伟,胡蕴玉,魏义勇,等.脂肪干细胞/Ⅰ型胶原凝胶复合体的构建及其体内外成骨分化研究[J].中华实验外科杂志,2007, 24(6):659.
[28] 连瑞玲,郭晓令,郭永龙,等.Maxadilan对人脂肪干细胞的影响[J].中国病理生理杂志,2015,31(3):475-480.
[29] 孙雅娟,李华,刘娜,等.Apical bud上皮诱导脂肪间充质干细胞向成牙本质样细胞分化的研究[J].中华老年口腔医学杂志,2015, 13(1):7-11.
[30] 刘爽,黄海森,于湄,等.外源性NO对脂肪干细胞成软骨分化的影响[J].四川大学学报（医学版）,2015,46(1):6-10.
[31] 陈冲,闫俊灵,李梁,等.人脂肪来源间充质干细胞的制备及其质量检验方法[J].武警医学,2015(2):170-174.
[32] 傅晓东,王伟力,沈奕,等.股骨近端微量元素Zn与骨形态发生蛋白7及Stro-1+细胞的相关性：预测髋关节假体寿命[J].中国组织工程研究,2014,18(44):7061-7065.
[33] 曾宪利,杨春露,李江,等.瓦楞状组织工程骨支架对种子细胞接种和成骨的影响[J].中国组织工程研究,2014,18(25):3953-3960.
[34] 刘晓阳,李广润,刘洪涛,等.硫酸钙人工骨/骨髓间充质干细胞构建组织工程化骨诱导脊柱融合[J].中国组织工程研究,2014, 18(21):3281-3286.
[35] 李俊辉,俞兴,穆晓红,等.人工颈椎间盘不同涂层影响骨髓间充质干细胞的黏附及分化[J].中国组织工程研究,2014,18(12): 1938-1943.
[36] 毕鑫,李多玉,杨毅,等.不同人工骨材料复合骨髓间充质干细胞治疗早期股骨头坏死：争议与进展[J].中国组织工程研究,2014, 18(12):1957-1962.
[37] Sugitani S, Tsuruma K, Ohno Y, et al. The potential neuroprotective effect of human adipose stem cells conditioned medium against light-induced retinal damage. Exp Eye Res. 2013;116:254-264.
[38] Wang W, Wang W, Jiang Y, et al. Human adipose-derived stem cells modified by HIF-1α accelerate the recovery of cisplatin-induced acute renal injury in vitro. Biotechnol Lett. 2014;36(3):667-676.
[39] Markarian CF, Frey GZ, Silveira MD, et al. Isolation of adipose-derived stem cells: a comparison among different methods. Biotechnol Lett. 2014;36(4):693-702.
[40] 万光勇.人脂肪干细胞与部分脱钙骨支架构建组织工程骨的试验研究[D].济南:山东大学,2009.
[41] 张胜利,邓展生.大鼠脂肪干细胞复合纳米羟基磷灰石/胶原/L-聚乳酸体内成骨的研究[J].重庆医学,2010,39(12):1533-1535.
[42] 史建国.人脂肪干细胞及生物可降解聚乳酸/胶原支架构建组织工程输尿管研究[D].北京:军医进修学院,2012.
[43] 察鹏飞.人细胞外基质支架联合bFGF-PLA纳米微球缓释系统对人脂肪来源干细胞构建工程化脂肪组织的影响研究[D].广州:南方医科大学,2012.
[44] 尹鹏,梁庆威.外源性骨形态发生蛋白7联合血管内皮生长因子诱导大鼠脂肪干细胞向成骨细胞分化的实验研究[J].中国医科大学学报,2011,40(10):897-901.
[45] Song K, Wang H, Wang H, et al. Investigation of the effective action distance between hematopoietic stem/progenitor cells and human adipose-derived stem cells during their in vitro co-culture. Appl Biochem Biotechnol. 2011;165(3-4):776-784.
[46] Shi LB, Cai HX, Chen LK, et al. Tissue engineered bulking agent with adipose-derived stem cells and silk fibroin microspheres for the treatment of intrinsic urethral sphincter deficiency. Biomaterials. 2014;35(5):1519-1530.
[47] 赵斌,马信龙,孙晓雷,等.兔坐骨神经匀浆上清液对脂肪干细胞的诱导分化作用[J].中国修复重建外科杂志,2015,29(4):477-482.
[48] 王楷,谢亮,李爱林.慢病毒介导SOX9基因转染小鼠脂肪干细胞的实验研究[J].中国美容整形外科杂志,2015,26(4):224-227.
[49] 刘林奇,鲁峰,王量,等.低氧预处理对人脂肪来源干细胞增殖和分化潜能影响的研究[J].中国美容整形外科杂志,2015,26(4): 231-234.
[50] 吴娟娟,田芳,宋琦.2种不同成牙本质诱导液对大鼠脂肪干细胞增殖凋亡的影响[J].重庆医学,2015,44(7):881-884.
[51] 刘振宁.GDF-5诱导脂肪干细胞复合胶原支架成软骨细胞分化修复椎间盘退变的前期实验性研究[D].沈阳:中国医科大学, 2008.
[52] 曹娜,裴路,张微.脂肪干细胞和生物支架应用于牙槽骨修复[J].中国组织工程研究,2014,01:137-142.
[53] 叶眉.脂肪干细胞增殖、成骨分化及其与支架生物相容性研究[D].合肥:安徽医科大学,2009.
[54] 孙威,倪新初,孙苏平,等.脂肪来源干细胞移植干预兔骨骼肌放射性纤维化的实验研究[J].中华放射医学与防护杂志,2015,35(3): 171-176.
[55] 俞富祥,黄立栋,唐银河,等.脂肪间质干细胞抑制大鼠胰腺星状细胞活性的实验研究[J].中华普通外科杂志,2015,30(4):304-307.
[56] 徐楠,孙强,王晨超,等.脂肪间充质干细胞移植治疗兔耳静脉淤血皮瓣的实验研究[J].中国美容整形外科杂志,2015,26(2):68-71.
[57] 周春光,段文彪,赵延荣,等.肝星状细胞对VEGF介导的脂肪间充质干细胞向内皮细胞分化的影响[J].肝胆胰外科杂志,2015, 27(2):125-132.
[58] 曹蕊,吕涛,严笠,等.两种培养基诱导人和大鼠脂肪干细胞向脂肪细胞分化的比较[J].中国比较医学杂志,2015,25(2):18-21.
[59] 阳水发,易阳艳.脂肪干细胞构建可注射型组织工程脂肪的研究进展[J].中国修复重建外科杂志,2015,29(2):245-249.